{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T21:04:44Z","timestamp":1788815084147,"version":"build-2803163510"},"reference-count":52,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2007,7,20]],"date-time":"2007-07-20T00:00:00Z","timestamp":1184889600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Experimental Allergy"],"published-print":{"date-parts":[[2007,9]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p><jats:bold>Background\n\t\t\t\t\t<\/jats:bold> The effect of ageing on several pathologic features of allergic asthma (pulmonary inflammation, eosinophilia, mucus hypersecretion), and their relationship with airway hyperresponsiveness (AHR) is not well characterized.<\/jats:p><jats:p><jats:bold>Objective\n\t\t\t\t\t<\/jats:bold> To evaluate lung inflammation, mucus metaplasia and AHR in relationship with age in murine models of allergic asthma comparing young and older mice.<\/jats:p><jats:p><jats:bold>Methods\n\t\t\t\t\t<\/jats:bold> Young (6 weeks) and older (6, 12, 18 months) BALB\/c mice were sensitized and challenged with ovalbumin (OVA). AHR and bronchoalveolar fluid (BALF), total inflammatory cell count and differential were measured. To evaluate mucus metaplasia, quantitative PCR for the major airway mucin\u2010associated gene, MUC\u20105AC, from lung tissue was measured, and lung tissue sections stained with periodic acid\u2010Schiff (PAS) for goblet\u2010cell enumeration. Lung tissue cytokine gene expression was determined by quantitative PCR, and systemic cytokine protein levels by ELISA from spleen\u2010cell cultures. Antigen\u2010specific serum IgE was determined by ELISA.<\/jats:p><jats:p><jats:bold>Results\n\t\t\t\t\t<\/jats:bold> AHR developed in both aged and young OVA\u2010sensitized\/challenged mice (OVA mice), and was more significantly increased in young OVA mice than in aged OVA mice. However, BALF eosinophil numbers were significantly higher, and lung histology showed greater inflammation in aged OVA mice than in young OVA mice. MUC\u20105AC expression and numbers of PAS+ staining bronchial epithelial cells were significantly increased in the aged OVA mice. All aged OVA mice had increased IL\u20105 and IFN\u2010\u03b3 mRNA expression in the lung and IL\u20105 and IFN\u2010\u03b3 protein levels from spleen cell cultures compared with young OVA mice. OVA\u2010IgE was elevated to a greater extent in aged OVA mice.<\/jats:p><jats:p><jats:bold>Conclusions\n\t\t\t\t\t<\/jats:bold> Although pulmonary inflammation and mucus metaplasia after antigen sensitization\/challenge occurred to a greater degree in older mice, the increase in AHR was significantly less compared with younger OVA mice. Antigen treatment produced a unique cytokine profile in older mice (elevated IFN\u2010\u03b3 and IL\u20105) compared with young mice (elevated IL\u20104 and IL\u201013). Thus, the airway response to inflammation is lessened in ageing animals, and may represent age\u2010associated events leading to different phenotypes in response to antigen provocation.<\/jats:p>","DOI":"10.1111\/j.1365-2222.2007.02775.x","type":"journal-article","created":{"date-parts":[[2007,7,20]],"date-time":"2007-07-20T12:06:51Z","timestamp":1184933211000},"page":"1392-1403","source":"Crossref","is-referenced-by-count":72,"title":["Effect of ageing on pulmonary inflammation, airway hyperresponsiveness and T and B cell responses in antigen\u2010sensitized and \u2010challenged mice"],"prefix":"10.1111","volume":"37","author":[{"given":"Paula J.","family":"Busse","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teng Fei","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kamal","family":"Srivastava","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian","family":"Schofield","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiu\u2010Min","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2007,7,20]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0091-6749(74)90001-3"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1378\/chest.116.3.603"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200202-090OC"},{"key":"e_1_2_6_5_2","first-page":"101","article-title":"Distinguishing severe asthma phenotypes","volume":"113","author":"Miranda C","year":"2004","journal-title":"role of age at onset and eosinophilic inflammation"},{"key":"e_1_2_6_6_2","first-page":"35","article-title":"Late onset asthma clinical and immunological data","volume":"8","author":"Ariano R","year":"1998","journal-title":"importance of allergy"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1159\/000029294"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1378\/chest.100.4.935"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.156.1.9608072"},{"key":"e_1_2_6_10_2","first-page":"4730","article-title":"Transplacental priming of the human immune system to environmental allergens","volume":"160","author":"Prescott SL","year":"1998","journal-title":"universal skewing of initial T cell responses toward the Th2 cytokine profile"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.162.supplement_2.ras-7"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.271.5256.1728"},{"key":"e_1_2_6_13_2","doi-asserted-by":"crossref","first-page":"4217","DOI":"10.4049\/jimmunol.160.9.4217","article-title":"Newborn mice develop balanced Th1\/Th2 primary effector responses in vivo but are biased to Th2 secondary responses","volume":"160","author":"Adkins B","year":"1998","journal-title":"J Immunol"},{"key":"e_1_2_6_14_2","first-page":"127","article-title":"Age\u2010associated alterations in splenic inos regulation","volume":"195","author":"Poynter ME","year":"1999","journal-title":"influence of constitutively expressed IFN-gamma and correction following supplementation with PPARalpha activators or vitamin E"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1006\/clim.2000.4894"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0047-6374(97)00153-X"},{"key":"e_1_2_6_17_2","first-page":"1289","article-title":"Polymorphism of age\u2010related changes in interleukin (IL) production","volume":"20","author":"Kubo M","year":"1990","journal-title":"differential changes of T helper subpopulations, synthesizing IL 2, IL 3 and IL 4"},{"key":"e_1_2_6_18_2","first-page":"297","article-title":"Interleukin\u20105 production by mononuclear cells from aged individuals","volume":"106","author":"Lio D","year":"1999","journal-title":"implication for autoimmunity"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.176.4.2546"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.173.2.1298"},{"key":"e_1_2_6_21_2","volume-title":"Guide for the care and use of laboratory animals. National Institutes of Health publication 86\u201023","author":"Institute of Laboratory Animal Resources Commissions and Life Sciences, NCR.","year":"1996"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1211\/0022357011775929"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.165.10.5906"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1067\/mai.2000.110102"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2004.01.243"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2005.08.059"},{"key":"e_1_2_6_27_2","doi-asserted-by":"crossref","first-page":"3216","DOI":"10.4049\/jimmunol.157.8.3216","article-title":"Mucosal IFN\u2010gamma gene transfer inhibits pulmonary allergic responses in mice","volume":"157","author":"Li XM","year":"1996","journal-title":"J Immunol"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1067\/mai.2001.119918"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2006.04.031"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/30.10.e48"},{"key":"e_1_2_6_31_2","first-page":"1049","article-title":"Inhibition of allergic airways inflammation and airway hyperresponsiveness in mice by dexamethasone","volume":"111","author":"Eum SY","year":"2003","journal-title":"role of eosinophils, IL-5, eotaxin, and IL-13"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.170.6.3296"},{"key":"e_1_2_6_33_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.167.10.5948"},{"key":"e_1_2_6_34_2","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb.22.3.3768"},{"issue":"1","key":"e_1_2_6_35_2","first-page":"76","article-title":"Atopic asthma is dominant in elderly onset asthmatics","volume":"120","author":"Atsuta R","year":"1999","journal-title":"possibility for an alteration of mast cell function by aging through Fc receptor expression"},{"key":"e_1_2_6_36_2","doi-asserted-by":"crossref","first-page":"396","DOI":"10.4049\/jimmunol.163.1.396","article-title":"Decreased expression of Th2 type cytokine mrna contributes to the lack of allergic bronchial inflammation in aged rats","volume":"163","author":"Ide K","year":"1999","journal-title":"J Immunol"},{"key":"e_1_2_6_37_2","doi-asserted-by":"publisher","DOI":"10.1080\/019021401750069401"},{"key":"e_1_2_6_38_2","first-page":"154","article-title":"The concept of telomeric non\u2010reciprocal recombination (TENOR) applied to human fibroblasts grown in serial cultures","volume":"8","author":"Hasenmaile S","year":"2005","journal-title":"concordance with genealogical data"},{"key":"e_1_2_6_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-6749(97)70298-7"},{"key":"e_1_2_6_40_2","first-page":"992","article-title":"Airway hyperresponsiveness in asthma","volume":"53","author":"Brusasco V","year":"1998","journal-title":"not just a matter of airway inflammation"},{"key":"e_1_2_6_41_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI17974"},{"key":"e_1_2_6_42_2","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb.26.2.4600"},{"key":"e_1_2_6_43_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.167.8.4668"},{"key":"e_1_2_6_44_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00380.2003"},{"key":"e_1_2_6_45_2","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200202-095OC"},{"key":"e_1_2_6_46_2","first-page":"1810","article-title":"Constitutive expressions of type I NOS in human airway smooth muscle cells","volume":"13","author":"Patel HJ","year":"1999","journal-title":"evidence for an antiproliferative role"},{"key":"e_1_2_6_47_2","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2005-0293OC"},{"key":"e_1_2_6_48_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mad.2004.05.007"},{"key":"e_1_2_6_49_2","first-page":"241","article-title":"Airway mucus hypersecretion in asthma","volume":"4","author":"Rogers DF.","year":"2004","journal-title":"an undervalued pathology?"},{"key":"e_1_2_6_50_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M210429200"},{"key":"e_1_2_6_51_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1099472"},{"key":"e_1_2_6_52_2","first-page":"463","article-title":"Structural changes in the airways in asthma","volume":"108","author":"Bai TR","year":"2005","journal-title":"observations and consequences"},{"key":"e_1_2_6_53_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.167.5.2798"}],"container-title":["Clinical &amp; Experimental Allergy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1365-2222.2007.02775.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1365-2222.2007.02775.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,13]],"date-time":"2023-10-13T18:02:02Z","timestamp":1697220122000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1365-2222.2007.02775.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,7,20]]},"references-count":52,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2007,9]]}},"alternative-id":["10.1111\/j.1365-2222.2007.02775.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1365-2222.2007.02775.x","archive":["Portico"],"relation":{},"ISSN":["0954-7894","1365-2222"],"issn-type":[{"value":"0954-7894","type":"print"},{"value":"1365-2222","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,7,20]]}}}