{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T16:22:56Z","timestamp":1770481376937,"version":"3.49.0"},"reference-count":32,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2008,8,3]],"date-time":"2008-08-03T00:00:00Z","timestamp":1217721600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Experimental Allergy"],"published-print":{"date-parts":[[2008,11]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p><jats:bold>Background\n\t\t\t\t\t<\/jats:bold> The addition of a nitric oxide (NO)\u2010releasing moiety to prednisolone was shown to enhance the anti\u2010inflammatory activity of this glucocorticoid in some experimental conditions, but its effectiveness in the context of eosinophilic inflammation remains to be elucidated.<\/jats:p><jats:p><jats:bold>Objective\n\t\t\t\t\t<\/jats:bold> This study compared the anti\u2010inflammatory effect of prednisolone to a NO\u2010releasing derivative of prednisolone, NCX\u20101015, using a model of allergen\u2010evoked eosinophil recruitment in rats. The efficacy of a NO\u2010donor compound, DETA\u2010NONOate, was also assessed for comparison.<\/jats:p><jats:p><jats:bold>Methods\n\t\t\t\t\t<\/jats:bold> Wistar rats were actively sensitized with Al(OH)<jats:sub>3<\/jats:sub> plus ovalbumin and 14 days later challenged with antigen intrapleurally. Treatments were performed locally 1\u2003h before challenge. Cysteinyl\u2010leucotrienes (Cys\u2010LT) and eotaxin were measured by ELISA.<\/jats:p><jats:p><jats:bold>Results\n\t\t\t\t\t<\/jats:bold> Antigen challenge induced an eosinophil infiltration at 12\u2003h, maximal at 24\u2003h. It also caused an increase in the levels of Cys\u2010LTs in the pleural exudate and in the expression of 5\u2010lipoxygenase (5\u2010LO) in infiltrated leucocytes at 6\u2003h, peaking at 12\u2003h and persisting for at least 24\u2003h. Treatment with equimolar doses of prednisolone and NCX\u20101015 inhibited the late eosinophil infiltration, although the dose required to produce maximal inhibition was about one\u2010tenth that of prednisolone. Cys\u2010LT generation and 5\u2010LO expression were inhibited by NCX\u20101015 but not by prednisolone. Treatment with prednisolone combined with the NO\u2010donor DETA\u2010NONOate led to a greater inhibition of the eosinophilia and Cys\u2010LT generation as compared with either drug alone. Administration of the steroid receptor antagonist RU 486, 1\u2003h before prednisolone and NCX\u20101015, abolished the inhibitory effect of the former, under conditions where it only partially affected the latter.<\/jats:p><jats:p><jats:bold>Conclusions\n\t\t\t\t\t<\/jats:bold> Our findings indicate that NCX\u20101015 provided a greater anti\u2010inflammatory effect than prednisolone on the allergic eosinophil recruitment in rats, suggesting that NO\u2010releasing steroids can be considered as a promising therapeutic approach to allergic diseases.<\/jats:p>","DOI":"10.1111\/j.1365-2222.2008.03083.x","type":"journal-article","created":{"date-parts":[[2008,8,4]],"date-time":"2008-08-04T00:58:17Z","timestamp":1217811497000},"page":"1830-1837","source":"Crossref","is-referenced-by-count":11,"title":["Suppressive effects of nitric oxide\u2010releasing prednisolone NCX\u20101015 on the allergic pleural eosinophil recruitment in rats"],"prefix":"10.1111","volume":"38","author":[{"given":"M. S. S.","family":"Oliveira","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"De O. Barreto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Zamuner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. L. A.","family":"Pires","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T. P. T.","family":"Ferreira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. S. B.","family":"Cordeiro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V.","family":"Lagente","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. A.","family":"Martins","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. L.","family":"Wallace","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P. M. 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