{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T19:19:04Z","timestamp":1792523944661,"version":"4.5.0"},"reference-count":16,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2009,8,26]],"date-time":"2009-08-26T00:00:00Z","timestamp":1251244800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Experimental Allergy"],"published-print":{"date-parts":[[2010,2]]},"abstract":"Summary<\/jats:title>Background\n\t\t\t\t\t<\/jats:bold> Component\u2010resolved diagnosis (CRD) using microarray technology has recently been introduced into the field of clinical allergology.<\/jats:p>Objective\n\t\t\t\t\t<\/jats:bold> To further validate the use of CRD by microarray technology in allergy diagnosis.<\/jats:p>Methods\n\t\t\t\t\t<\/jats:bold> Thiry\u2010seven patients allergic to birch pollen were included. The discriminative value of apple\u2010specific IgE (sIgE), recombinant Mal d 1 (rMal d 1) sIgE, apple skin prick test and rMal d 1 on the microarray was assessed between patients with a birch\u2010related oral allergy syndrome to apple (OAS+<\/jats:sup>, n<\/jats:italic>=20) and healthy control individuals (HC, n<\/jats:italic>=8) without a history of inhalant allergies or apple\u2010induced OAS. An additional comparative analysis was carried out with individuals allergic to birch pollen allergy without OAS (OAS\u2212<\/jats:sup>; n<\/jats:italic>=17).<\/jats:p>Results\n\t\t\t\t\t<\/jats:bold> rMal d 1 coupled to the microarray constitutes a discriminative marker between OAS+<\/jats:sup> and HC with a sensitivity 95% and a specificity of 100%. However, in parallel with the traditional sIgE assay, 15 out of 17 OAS\u2212<\/jats:sup> individuals (88%) also displayed IgE reactivity to rMal d 1 coupled to the microarray. OAS\u2212<\/jats:sup> individuals are more frequently sensitized to mite (about three to four times), cat and dog dander (about two to three times) and grass pollen (about 1.5 times) as compared with OAS+<\/jats:sup> patients.<\/jats:p>Conclusion\n\t\t\t\t\t<\/jats:bold> At first glance, CRD by microarray seems to be a reliable instrument in the diagnosis of apple\u2010mediated OAS in birch pollen allergy. However, for discriminating between sensitization and a real allergy, micro\u2010arrayed rMal d 1 offers no advantage over conventional quantification of rMal d 1 sIgE. Most interestingly, within a single run, birch pollen\u2010allergic patients without OAS to apple were shown to display a broader sensitization to classical inhalant allergens than birch pollen\u2010allergic patients with an apple\u2010related OAS.<\/jats:p> Cite this as<\/jats:italic>: D. G. Ebo, C. H. Bridts, M. M. Verweij, K. J. De Knop, M. M. Hagendorens, L. S. De Clerck and W. J. Stevens, Clinical & Experimental Allergy<\/jats:italic>, 2010 (40) 339\u2013 347.<\/jats:p>","DOI":"10.1111\/j.1365-2222.2009.03345.x","type":"journal-article","created":{"date-parts":[[2009,8,26]],"date-time":"2009-08-26T09:32:21Z","timestamp":1251279141000},"page":"339-347","source":"Crossref","is-referenced-by-count":66,"title":["Sensitization profiles in birch pollen\u2010allergic patients with and without oral allergy syndrome to apple: lessons from multiplexed component\u2010resolved allergy diagnosis"],"prefix":"10.1111","volume":"40","author":[{"given":"D. G.","family":"Ebo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. H.","family":"Bridts","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. M.","family":"Verweij","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K. J.","family":"De Knop","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. M.","family":"Hagendorens","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L. S.","family":"De Clerck","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W. J.","family":"Stevens","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2010,1,11]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1159\/000234488"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2222.1999.00653.x"},{"key":"e_1_2_6_4_2","first-page":"259","article-title":"Recombinant marker allergens","volume":"127","author":"Kazemi\u2010Shirazi L","year":"2002","journal-title":"diagnostic gatekeepers for the treatment of allergy"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.all.0000225166.90768.d6"},{"key":"e_1_2_6_6_2","first-page":"125","article-title":"Allergy testing","volume":"44","author":"Mothes N","year":"2006","journal-title":"the role of recombinant allergens"},{"key":"e_1_2_6_7_2","first-page":"1443","article-title":"Allergen microarray","volume":"33","author":"Jahn\u2010Schmid B","year":"2003","journal-title":"comparison of microarray using recombinant allergens with conventional diagnostic methods to detect allergen-specific serum immunoglobulin"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2006.01078.x"},{"key":"e_1_2_6_9_2","first-page":"1321","article-title":"Protein microarrays for the diagnosis of allergic diseases","volume":"43","author":"Harwanegg C","year":"2005","journal-title":"state-of-the-art and future development"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2008.01748.x"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2008.01805.x"},{"key":"e_1_2_6_12_2","first-page":"481","article-title":"Apple allergy across Europe","volume":"118","author":"Fernandez\u2010Rivas M","year":"2006","journal-title":"how allergen sensitization profiles determine the clinical expression of allergies to plant foods"},{"key":"e_1_2_6_13_2","first-page":"435","article-title":"IgE to Bet v 1 and profilin","volume":"110","author":"Wensing M","year":"2002","journal-title":"cross-reactivity patterns and clinical relevance"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.20042"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2006.01078.x"},{"key":"e_1_2_6_16_2","first-page":"137","article-title":"Sensitization to cross\u2010reactive carbohydrate determinants and the ubiquitous protein profilin","volume":"34","author":"Ebo DG","year":"2004","journal-title":"mimickers of allergy"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1067\/mai.2001.118515"}],"container-title":["Clinical & Experimental Allergy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1365-2222.2009.03345.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1365-2222.2009.03345.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,31]],"date-time":"2023-10-31T00:52:45Z","timestamp":1698713565000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1365-2222.2009.03345.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,1,11]]},"references-count":16,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2010,2]]}},"alternative-id":["10.1111\/j.1365-2222.2009.03345.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1365-2222.2009.03345.x","archive":["Portico"],"relation":{},"ISSN":["0954-7894","1365-2222"],"issn-type":[{"value":"0954-7894","type":"print"},{"value":"1365-2222","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,1,11]]}}}