{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,17]],"date-time":"2025-11-17T14:10:10Z","timestamp":1763388610888},"reference-count":17,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2009,10,30]],"date-time":"2009-10-30T00:00:00Z","timestamp":1256860800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Experimental Allergy"],"published-print":{"date-parts":[[2010,2]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p><jats:bold>Background\n\t\t\t\t\t<\/jats:bold> Assessment of airway inflammation in asthma is becoming increasingly important, as the inflammatory phenotype underpins the treatment response.<\/jats:p><jats:p><jats:bold>Objective\n\t\t\t\t\t<\/jats:bold> This study aimed to evaluate mannitol as a tool for assessing airway responsiveness and airway inflammation in asthma, compared with hypertonic saline.<\/jats:p><jats:p><jats:bold>Methods\n\t\t\t\t\t<\/jats:bold> Fifty\u2010five subjects with stable asthma completed a hypertonic (4.5%) saline challenge and a mannitol challenge at two separate visits, performed 48\u201372\u2003h apart, in random order.<\/jats:p><jats:p><jats:bold>Results\n\t\t\t\t\t<\/jats:bold> Induced sputum was obtained from 49 (89%) subjects during the saline challenge and 42 (76%) subjects during the mannitol challenge (<jats:italic>P<\/jats:italic>&gt;0.05). There was a significant correlation between the greatest percentage fall in forced expiratory volume in 1\u2003s (FEV<jats:sub>1<\/jats:sub>) (<jats:italic>r<\/jats:italic>=0.6, <jats:italic>P<\/jats:italic>&lt;0.0001), the dose\u2013response slope (<jats:italic>r<\/jats:italic>=0.73), cumulative dose (<jats:italic>r<\/jats:italic>=0.55) and PD15 (<jats:italic>r<\/jats:italic>=0.46) for mannitol and hypertonic saline. The greatest percentage fall in FEV<jats:sub>1<\/jats:sub> to mannitol was less in non\u2010eosinophilic asthma. There was a lower total cell count in mannitol vs. hypertonic\u2010saline\u2010induced sputum. However, sputum eosinophils and neutrophils were not significantly different. Using mannitol, a higher proportion of subjects were classified as having eosinophilic asthma. There were no differences in IL\u20108, neutrophil elastase or matrix\u2010metalloproteinase 9 concentrations in sputum samples induced with mannitol or hypertonic saline.<\/jats:p><jats:p><jats:bold>Conclusion\n\t\t\t\t\t<\/jats:bold> We conclude that mannitol can be used to induce good\u2010quality sputum, useful for analysis of inflammatory mediators and for predicting the inflammatory phenotype in asthma.<\/jats:p><jats:p> <jats:italic>Cite this as<\/jats:italic>: L. G. Wood, H. Powell and P. G. Gibson, <jats:italic>Clinical &amp; Experimental Allergy<\/jats:italic>, 2010 (40) 232\u2013241.<\/jats:p>","DOI":"10.1111\/j.1365-2222.2009.03371.x","type":"journal-article","created":{"date-parts":[[2009,10,30]],"date-time":"2009-10-30T04:03:07Z","timestamp":1256875387000},"page":"232-241","source":"Crossref","is-referenced-by-count":41,"title":["Mannitol challenge for assessment of airway responsiveness, airway inflammation and inflammatory phenotype in asthma"],"prefix":"10.1111","volume":"40","author":[{"given":"L. G.","family":"Wood","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Powell","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P. G.","family":"Gibson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,1,11]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200304-503OC"},{"key":"e_1_2_6_3_2","first-page":"1433","article-title":"Efficacy of a short course of parent\u2010initiated oral prednisolone for viral wheeze in children aged 1\u20135 years","volume":"362","author":"Oommen A","year":"2003","journal-title":"randomised controlled trial"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.02.00226302"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.159.4.9806052"},{"key":"e_1_2_6_6_2","first-page":"1715","article-title":"Asthma exacerbations and sputum eosinophil counts","volume":"360","author":"Green RH","year":"2002","journal-title":"a randomised controlled trial"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm\/143.3_Pt_2.S43"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.158.1.9705031"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200503-369OC"},{"key":"e_1_2_6_10_2","first-page":"1008","article-title":"Noninvasive assessment of airway inflammation in children","volume":"16","author":"Gibson PG","year":"2000","journal-title":"induced sputum, exhaled nitric oxide, and breath condensate"},{"key":"e_1_2_6_11_2","first-page":"54","article-title":"Inflammatory subtypes in asthma","volume":"11","author":"Simpson JL","year":"2006","journal-title":"assessment and identification using induced sputum"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.283.6302.1285"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.06.00078205"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.156.3.9701113"},{"key":"e_1_2_6_15_2","first-page":"144","article-title":"The safety and efficacy of inhaled dry powder mannitol as a bronchial provocation test for airway hyperresponsiveness","volume":"6","author":"Brannan JD","year":"2005","journal-title":"a phase 3 comparison study with hypertonic (4.5%) saline"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1440-1843.2002.00357.x"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.163.2.9912091"},{"key":"e_1_2_6_18_2","first-page":"322","article-title":"Fractional analysis of sequential induced sputum samples during sputum induction","volume":"104","author":"Gershman NH","year":"1999","journal-title":"evidence that different lung compartments are sampled at different time points"}],"container-title":["Clinical &amp; Experimental Allergy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1365-2222.2009.03371.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1365-2222.2009.03371.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,31]],"date-time":"2023-10-31T00:52:37Z","timestamp":1698713557000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1365-2222.2009.03371.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,1,11]]},"references-count":17,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2010,2]]}},"alternative-id":["10.1111\/j.1365-2222.2009.03371.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1365-2222.2009.03371.x","archive":["Portico"],"relation":{},"ISSN":["0954-7894","1365-2222"],"issn-type":[{"value":"0954-7894","type":"print"},{"value":"1365-2222","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,1,11]]}}}