{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T04:29:21Z","timestamp":1788496161020,"version":"build-2803163510"},"reference-count":32,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2009,11,13]],"date-time":"2009-11-13T00:00:00Z","timestamp":1258070400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Experimental Allergy"],"published-print":{"date-parts":[[2009,12]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p><jats:bold>Background\n\t\t\t\t\t<\/jats:bold> Patients allergic to mustard are frequently sensitized to peach.<\/jats:p><jats:p><jats:bold>Objective\n\t\t\t\t\t<\/jats:bold> To identify and analyse new yellow mustard allergens that could be involved in IgE cross\u2010reactivity.<\/jats:p><jats:p><jats:bold>Methods\n\t\t\t\t\t<\/jats:bold> Sera from mustard\u2010allergic patients with symptoms to peach were studied. Mass spectrometry analyses provided sequences of IgE\u2010reactive proteins. cDNAs encoding Sin a 3 and Sin a 4 were amplified by polymerase chain reaction, cloned and sequenced. The recombinant allergens were obtained in <jats:italic>Pichia pastoris<\/jats:italic> and <jats:italic>Escherichia coli<\/jats:italic>, respectively, and used for ELISA, immunoblotting and inhibition experiments. Sequence alignment was used to identify common IgE epitopes.<\/jats:p><jats:p><jats:bold>Results\n\t\t\t\t\t<\/jats:bold> Sin a 3\u2010 and Sin a 4\u2010specific cDNAs encode for mature proteins of 92 and 131 amino acids that belong to nsLTP and profilin protein families, respectively. Sin a 3 and Sin a 4 showed 54% and 80% identity with allergenic nsLTP from peach and profilin from melon, respectively. Both recombinant allergens were IgE\u2010reactive in ELISA and immunoblotting. Peach pulp and peel, and melon extracts nearly abolished the IgE binding to recombinant Sin a 3 or recombinant Sin a 4 in immunoblotting.<\/jats:p><jats:p><jats:bold>Conclusion\n\t\t\t\t\t<\/jats:bold> Sin a 3 (nsLTP) and Sin a 4 (profilin) were identified as new mustard allergens and showed IgE cross\u2010reactivity with fruits such as peach or melon, respectively. The knowledge of these two allergens will contribute towards better understand with cross\u2010reactivity between mustard and other plant food allergens, and their availability will provide physicians with useful tools for molecular diagnosis.<\/jats:p>","DOI":"10.1111\/j.1365-2222.2009.03382.x","type":"journal-article","created":{"date-parts":[[2009,11,13]],"date-time":"2009-11-13T07:58:38Z","timestamp":1258099118000},"page":"1929-1936","source":"Crossref","is-referenced-by-count":41,"title":["nsLTP and profilin are allergens in mustard seeds: cloning, sequencing and recombinant production of Sin a 3 and Sin a 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