{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T00:54:59Z","timestamp":1789606499422,"version":"build-2803163510"},"reference-count":21,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2009,12,2]],"date-time":"2009-12-02T00:00:00Z","timestamp":1259712000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Experimental Allergy"],"published-print":{"date-parts":[[2010,4]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p><jats:bold>Background\n\t\t\t\t\t<\/jats:bold> One of the concerns of allergen\u2010specific immunotherapy is the possible boost of inflammatory allergen\u2010specific T lymphocytes. To address this problem, treatment with B cell epitopes devoid of allergen\u2010specific T cell epitopes would be a promising alternative.<\/jats:p><jats:p><jats:bold>Objective\n\t\t\t\t\t<\/jats:bold> In this study, we examined the therapeutic potency of a single mimotope, mimicking a structural IgE epitope of grass pollen allergen Phl p 5 in an established memory mouse model of acute allergic asthma.<\/jats:p><jats:p><jats:bold>Methods\n\t\t\t\t\t<\/jats:bold> In the experimental set\u2010up, BALB\/c mice were primed with intraperitoneal injections of recombinant Phl p 5a (rPhl p 5a) and subsequently aerosol challenged with the nebulized allergen. Mice developed signs of bronchial asthma including hypereosinophilia around bronchi, goblet cell hyperplasia and enhanced mucus production.<\/jats:p><jats:p><jats:bold>Results\n\t\t\t\t\t<\/jats:bold> When the mice were subsequently treated with the grass pollen mimotope coupled to keyhole limpet haemocyanin, bronchial eosinophilic inflammation and mucus hypersecretion decreased. Further, a decrease of Th2 cytokines IL\u20104 and IL\u20105 could be observed in the bronchoalveolar lavage (BAL). In contrast to rPhl p 5a, the mimotope was <jats:italic>in vitro<\/jats:italic> not able to stimulate splenocytes to proliferation or IL\u20105 production. Despite not affecting the levels of pre\u2010existing IgE, vaccination with the single mimotope thus rendered anti\u2010inflammatory effects in a mouse model of acute asthma.<\/jats:p><jats:p><jats:bold>Conclusion\n\t\t\t\t\t<\/jats:bold> From our data, we conclude that vaccination with a mimotope peptide representing a single IgE epitope of the allergen Phl p 5a and being devoid of allergen\u2010specific T cell epitopes is able to down\u2010regulate inflammation in acute asthma.<\/jats:p><jats:p> <jats:italic>Cite this as<\/jats:italic>: J. Wallmann, M. M. Epstein, P. Singh, R. Brunner, K. Szalai, L. El\u2010Housseiny, I. Pali\u2010Sch\u00f6ll and E. Jensen\u2010Jarolim, <jats:italic>Clinical &amp; Experimental Allergy<\/jats:italic>, 2010 (40) 650\u2013658.<\/jats:p>","DOI":"10.1111\/j.1365-2222.2009.03392.x","type":"journal-article","created":{"date-parts":[[2009,12,2]],"date-time":"2009-12-02T05:53:44Z","timestamp":1259733224000},"page":"650-658","source":"Crossref","is-referenced-by-count":18,"title":["Mimotope vaccination for therapy of allergic asthma: anti\u2010inflammatory effects in a mouse model"],"prefix":"10.1111","volume":"40","author":[{"given":"J.","family":"Wallmann","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. 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