{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,16]],"date-time":"2025-12-16T11:48:32Z","timestamp":1765885712205},"reference-count":33,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2009,12,8]],"date-time":"2009-12-08T00:00:00Z","timestamp":1260230400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Experimental Allergy"],"published-print":{"date-parts":[[2010,3]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p><jats:bold>Background\n\t\t\t\t\t<\/jats:bold> Allergic rhinitis (AR) is a very common disease and a risk factor for allergic asthma. The discovery of new biomarkers for the early detection of AR would improve the clinical outcomes and reduce socio\u2010economic burden. We sought to identify a novel serologic marker for detection of AR using a proteomic approach.<\/jats:p><jats:p><jats:bold>Methods\n\t\t\t\t\t<\/jats:bold> To identify the proteins involved in AR, comparative proteomics was applied using nasal lavage fluids (NLFs) taken before and after a nasal provocation test (NPT) with <jats:italic>Dermatophagoides pteronyssinus<\/jats:italic> (<jats:italic>Dpt<\/jats:italic>) in a subject with AR sensitized to <jats:italic>Dpt<\/jats:italic>. The clinical relevance of the identified proteins was evaluated by ELISA using NLFs and sera from the three study groups: <jats:italic>Dpt<\/jats:italic>\u2010sensitive AR; asymptomatic <jats:italic>Dpt<\/jats:italic>\u2010sensitive controls; and non\u2010atopic healthy controls. The sensitivities and specificities of the candidate proteins for predicting AR were determined using receiver operating characteristic (ROC) curves.<\/jats:p><jats:p><jats:bold>Results\n\t\t\t\t\t<\/jats:bold> In proteomic analysis, lactoferrin expression was up\u2010regulated after NPT. The validation study using ELISA showed a significantly lower serum lactoferrin level in the AR group than those of the other two groups (<jats:italic>P<\/jats:italic>&lt;0.05, respectively). To discriminate between subjects with or without AR, the optimal serum cut\u2010off level of lactoferrin was set at &lt;307\u2003ng\/mL using the ROC curve. The sensitivity and specificity for predicting AR were 81.4% and 58%. When combined with serum <jats:italic>Dpt<\/jats:italic>\u2010specific IgE level, the sensitivity and specificity for predicting AR were 76.7% and 79.2%.<\/jats:p><jats:p><jats:bold>Conclusion\n\t\t\t\t\t<\/jats:bold> These results suggest that the serum lactoferrin level is associated with the phenotype of <jats:italic>Dpt<\/jats:italic>\u2010sensitive AR, and in combination with the serum <jats:italic>Dpt<\/jats:italic>\u2010specific IgE level, may be a potential serologic marker for early detection of AR.<\/jats:p><jats:p> <jats:italic>Cite this as<\/jats:italic>: G.\u2010S. Choi, S.\u2010Y. Shin, J.\u2010H. Kim, H.\u2010Y. Lee, N. S. Palikhe, Y.\u2010M. Ye, S.\u2010H. Kim and H.\u2010S. Park, <jats:italic>Clinical &amp; Experimental Allergy<\/jats:italic>, 2010 (40) 403\u2013410.<\/jats:p>","DOI":"10.1111\/j.1365-2222.2009.03414.x","type":"journal-article","created":{"date-parts":[[2010,2,8]],"date-time":"2010-02-08T07:47:42Z","timestamp":1265615262000},"page":"403-410","source":"Crossref","is-referenced-by-count":15,"title":["Serum lactoferrin level as a serologic biomarker for allergic rhinitis"],"prefix":"10.1111","volume":"40","author":[{"given":"G. \u2010S.","family":"Choi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. \u2010Y.","family":"Shin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. \u2010H.","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. \u2010Y.","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N. 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