{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T07:55:15Z","timestamp":1791014115183,"version":"4.1.0"},"reference-count":50,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2011,3,21]],"date-time":"2011-03-21T00:00:00Z","timestamp":1300665600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Experimental Allergy"],"published-print":{"date-parts":[[2011,6]]},"abstract":"<jats:sec><jats:label\/><jats:p> <jats:italic>Cite this as<\/jats:italic>: M. A. Hofmann Bowman, A. Heydemann, J. Gawdzik, R. A. Shilling and B. Camoretti\u2010Mercado, <jats:italic>Clinical &amp; Experimental Allergy<\/jats:italic>, 2011 (41) 878\u2013889.<\/jats:p><\/jats:sec><jats:sec><jats:title>Summary<\/jats:title><jats:p><jats:bold>Background\n\t\t\t\t\t<\/jats:bold> The calcium\u2010binding protein S100A12 is highly up\u2010regulated in the serum and sputum of patients with allergic asthma and is suggested to be a biomarker and pathologic mediator of asthma.<\/jats:p><jats:p><jats:bold>Objective\n\t\t\t\t\t<\/jats:bold> To test the role of S100A12 in mediating airway inflammation in a mouse model of allergic lung inflammation.<\/jats:p><jats:p><jats:bold>Methods\n\t\t\t\t\t<\/jats:bold> Transgenic (TG) mice that express human S100A12 and wild\u2010type (WT) littermates were sensitized and challenged with ovalbumin (OVA) and assessed for inflammation, lung structure, and function.<\/jats:p><jats:p><jats:bold>Results\n\t\t\t\t\t<\/jats:bold> Following OVA sensitization and challenge, S100A12 TG mice showed reduced peribronchial and perivascular inflammation, mucus production, and eosinophilia as well as attenuated airway responsiveness to contractile agonist compared with WT sensitized and challenged animals. This is explained, at least in part, by remodelled airways in S100A12 TG mice with thinning of the airway smooth muscle. S100A12 exposure induced Fas expression and activation of caspase 3 in cultured airway smooth muscle cells, suggesting that airway smooth muscle abnormalities observed in S100A12 TG mice may be mediated through myocyte apoptosis.<\/jats:p><jats:p><jats:bold>Conclusion and Clinical Relevance\n\t\t\t\t\t<\/jats:bold> S100A12 is one of the most abundant proteins found in the airways of human asthmatics, and it was postulated that S100A12 could mediate the inflammatory process. Our study shows for the first time that TG expression of S100A12 in the lung of mice does not exacerbate lung inflammation in a model of OVA\u2010induced allergic inflammation. We speculate that the high levels of S100\/calgranulins found in bronchoalveolar lavage fluid of asthmatics and of OVA\u2010treated TG S100A12 mice do not significantly mediate pulmonary inflammation.<\/jats:p><\/jats:sec>","DOI":"10.1111\/j.1365-2222.2011.03714.x","type":"journal-article","created":{"date-parts":[[2011,3,21]],"date-time":"2011-03-21T15:15:18Z","timestamp":1300720518000},"page":"878-889","source":"Crossref","is-referenced-by-count":28,"title":["Transgenic expression of human S100A12 induces structural airway abnormalities and limited lung inflammation in a mouse model of allergic inflammation"],"prefix":"10.1111","volume":"41","author":[{"given":"M. A.","family":"Hofmann Bowman","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Heydemann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Gawdzik","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. A.","family":"Shilling","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Camoretti\u2010Mercado","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2011,3,21]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M500041-MCP200"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4020-6191-2_5"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/keg385"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1136\/gut.52.6.847"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(03)12986-8"},{"key":"e_1_2_7_7_2","first-page":"106","article-title":"S100A12 provokes mast cell activation","volume":"119","author":"Yang Z","year":"2007","journal-title":"a potential amplification pathway in asthma and innate immunity"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.rmed.2007.11.011"},{"key":"e_1_2_7_9_2","first-page":"889","article-title":"RAGE mediates a novel proinflammatory axis","volume":"97","author":"Hofmann MA","year":"1999","journal-title":"a central cell surface receptor for S100\/calgranulin polypeptides"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm1638"},{"key":"e_1_2_7_11_2","first-page":"1699","article-title":"Survey of the distribution of a newly characterized receptor for advanced glycation end products in tissues","volume":"143","author":"Brett J","year":"1993","journal-title":"Am J Pathol"},{"key":"e_1_2_7_12_2","first-page":"475","article-title":"Promotion of cell adherence and spreading","volume":"323","author":"Demling N","year":"2006","journal-title":"a novel function of RAGE, the highly selective differentiation marker of human alveolar epithelial type I cells"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1017\/S146239940900101X"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI32703"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2008.070569"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0801199"},{"key":"e_1_2_7_17_2","first-page":"334","article-title":"Computational searches for missing orthologs","volume":"8","author":"Fuellen G","year":"2004","journal-title":"the case of S100A12 in mice"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.22.13460"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.109.209486"},{"key":"e_1_2_7_20_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20051680"},{"key":"e_1_2_7_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.nmd.2005.05.004"},{"key":"e_1_2_7_22_2","first-page":"353","article-title":"Mounier\u2013Kuhn syndrome","volume":"188","author":"Noori F","year":"2010","journal-title":"a case report"},{"key":"e_1_2_7_23_2","first-page":"191","article-title":"Regulation of TNF\u2010alpha\u2010 and IFN\u2010gamma\u2010induced CXCL10 expression","volume":"18","author":"Hardaker EL","year":"2004","journal-title":"participation of the airway smooth muscle in the pulmonary inflammatory response in chronic obstructive pulmonary disease"},{"key":"e_1_2_7_24_2","doi-asserted-by":"crossref","first-page":"29101","DOI":"10.1074\/jbc.M109.099952","article-title":"TNF\u2010alpha and IFN\u2010gamma synergistically enhance transcriptional activation of CXCL10 in human airway smooth muscle cells via STAT\u20101, NF\u2010kappaB, and the transcriptional coactivator CREB\u2010binding protein","volume":"285","author":"Clarke DL","journal-title":"J Biol Chem"},{"key":"e_1_2_7_25_2","first-page":"1548","article-title":"CXCR3 expression and activation of eosinophils","volume":"165","author":"Jinquan T","year":"2000","journal-title":"role of IFN-gamma-inducible protein-10 and monokine induced by IFN-gamma"},{"key":"e_1_2_7_26_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.168.10.5278"},{"key":"e_1_2_7_27_2","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.26.753"},{"key":"e_1_2_7_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2007.10.015"},{"key":"e_1_2_7_29_2","first-page":"93","article-title":"S100B interaction with the receptor for advanced glycation end products (RAGE)","volume":"106","author":"Tsoporis JN","year":"2010","journal-title":"a novel receptor-mediated mechanism for myocyte apoptosis postinfarction"},{"key":"e_1_2_7_30_2","first-page":"1437","article-title":"The receptor for advanced glycation end products and its ligands","volume":"19","author":"Morbini P","year":"2006","journal-title":"a new inflammatory pathway in lung disease?"},{"key":"e_1_2_7_31_2","doi-asserted-by":"publisher","DOI":"10.1038\/86867"},{"key":"e_1_2_7_32_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10038-007-0118-5"},{"key":"e_1_2_7_33_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.14.9496"},{"key":"e_1_2_7_34_2","doi-asserted-by":"publisher","DOI":"10.1586\/14789450.5.1.9"},{"key":"e_1_2_7_35_2","first-page":"743","article-title":"S100A8\/A9","volume":"87","author":"Halayko AJ","year":"2009","journal-title":"a mediator of severe asthma pathogenesis and morbidity?"},{"key":"e_1_2_7_36_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.pathmechdis.1.110304.100213"},{"key":"e_1_2_7_37_2","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm\/148.3.720"},{"key":"e_1_2_7_38_2","first-page":"248","article-title":"Airway smooth muscle in bronchial tone, inflammation, and remodeling","volume":"177","author":"Panettieri RA","year":"2008","journal-title":"basic knowledge to clinical relevance"},{"key":"e_1_2_7_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.trsl.2009.06.008"},{"key":"e_1_2_7_40_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0900180"},{"key":"e_1_2_7_41_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.177.10.7312"},{"key":"e_1_2_7_42_2","doi-asserted-by":"publisher","DOI":"10.1021\/pr049829t"},{"key":"e_1_2_7_43_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200800643"},{"key":"e_1_2_7_44_2","doi-asserted-by":"publisher","DOI":"10.1186\/1465-9921-11-107"},{"key":"e_1_2_7_45_2","doi-asserted-by":"publisher","DOI":"10.1159\/000142040"},{"key":"e_1_2_7_46_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1440-1711.2001.00981.x"},{"key":"e_1_2_7_47_2","first-page":"959","article-title":"Mouse model of airway remodeling","volume":"168","author":"Shinagawa K","year":"2003","journal-title":"strain differences"},{"key":"e_1_2_7_48_2","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.110.217745"},{"key":"e_1_2_7_49_2","doi-asserted-by":"publisher","DOI":"10.1096\/fj.07-9909com"},{"key":"e_1_2_7_50_2","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.06-0738"},{"key":"e_1_2_7_51_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0900373"}],"container-title":["Clinical &amp; Experimental Allergy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1365-2222.2011.03714.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1365-2222.2011.03714.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,16]],"date-time":"2023-10-16T03:10:32Z","timestamp":1697425832000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1365-2222.2011.03714.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,3,21]]},"references-count":50,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2011,6]]}},"alternative-id":["10.1111\/j.1365-2222.2011.03714.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1365-2222.2011.03714.x","archive":["Portico"],"relation":{},"ISSN":["0954-7894","1365-2222"],"issn-type":[{"value":"0954-7894","type":"print"},{"value":"1365-2222","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,3,21]]}}}