{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T12:13:47Z","timestamp":1768911227351,"version":"3.49.0"},"reference-count":41,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2011,7,5]],"date-time":"2011-07-05T00:00:00Z","timestamp":1309824000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Experimental Allergy"],"published-print":{"date-parts":[[2011,10]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>\n            <jats:bold>Background <\/jats:bold> The syndrome of allergic asthma features reversible bronchoconstriction, airway inflammation and hyperresponsiveness as well as airway remodelling, including goblet cell hyperplasia. Managing severe asthma is still a clinical challenge. Numerous studies report that furosemide, an inhibitor of Na<jats:sup>+<\/jats:sup>\u2013K<jats:sup>+<\/jats:sup>\u2013Cl<jats:sup>\u2212<\/jats:sup> cotransporter (NKCC) reduces airway hyperresponsiveness (AHR) in asthmatic patients. However, the mechanism by which furosemide exerts anti\u2010asthmatic action remains unclear.<\/jats:p><jats:p>\n            <jats:bold>Objective <\/jats:bold> This study sought to investigate the cellular profile of NKCC1 expression in the lung and examine the effects of furosemide on several outcome measurements in a mouse model of allergic asthma.<\/jats:p><jats:p>\n            <jats:bold>Methods <\/jats:bold> Mice were sensitized and challenged with ovalbumin (OVA). Before challenge, the OVA\u2010sensitized mice were treated with furosemide (4.0\u2003mg\/kg\/day, via daily intraperitoneal injection for 5 days). Outcome measurements in na\u00efve, OVA\u2010exposure, furosemide\u2010treated na\u00efve and furosemide\u2010treated OVA\u2010exposed mice included the slope of the relationship between inhaled methacholine (MCh) concentration and respiratory system resistance (<jats:italic>Slope\u00b7R<\/jats:italic><jats:sub>RS<\/jats:sub>), bronchoalveolar lavage (BAL) cell counts and immunohistochemical and immunoblotting assays of lung tissues.<\/jats:p><jats:p>\n            <jats:bold>Results <\/jats:bold> NKCC1 immunoreactivity was observed in airway epithelial cells (AECs) and alveolar type II (ATII) cells of the control mice. OVA exposure enhanced the expression of NKCC1 in AECs and ATII cells, and increased the infiltration of NKCC1\u2010expressing T lymphocytes in the lung. NKCC1 immunoreactivity was not detected in the airway smooth muscle (ASM) cells. Furosemide treatment reduced the <jats:italic>Slope\u00b7R<\/jats:italic><jats:sub>RS<\/jats:sub> in both na\u00efve and OVA\u2010exposed mice by about 50%. Furosemide treatment also increased T lymphocyte infiltration to the lung in OVA\u2010exposed mice by approximately 53%, but had no effect on pulmonary goblet cell hyperplasia.<\/jats:p><jats:p>\n            <jats:bold>Conclusions and Clinical Relevance <\/jats:bold> Furosemide decreases basal airway responsiveness, thereby reducing the extent of allergen\u2010induced AHR. However, the same treatment also increases T lymphocytes infiltration in the course of allergic asthma. Further studies are necessary to address the usefulness of furosemide in the clinical treatment of asthma.<\/jats:p><jats:p>\n            <jats:italic>Cite this as<\/jats:italic>: S. Wang, Y.\u2010Y. Xiang, R. Ellis, J. Wattie, M. Feng, M. D. Inman and W.\u2010Y. Lu, <jats:italic>Clinical &amp; Experimental Allergy<\/jats:italic>, 2011 (41) 1456\u20131467.<\/jats:p>","DOI":"10.1111\/j.1365-2222.2011.03811.x","type":"journal-article","created":{"date-parts":[[2011,7,6]],"date-time":"2011-07-06T03:46:50Z","timestamp":1309924010000},"page":"1456-1467","source":"Crossref","is-referenced-by-count":16,"title":["Effects of furosemide on allergic asthmatic responses in mice"],"prefix":"10.1111","volume":"41","author":[{"given":"S.","family":"Wang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y.\u2010Y.","family":"Xiang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Ellis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Wattie","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Feng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. 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