{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T14:21:18Z","timestamp":1776262878268,"version":"3.50.1"},"reference-count":42,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2011,9,1]],"date-time":"2011-09-01T00:00:00Z","timestamp":1314835200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Experimental Allergy"],"published-print":{"date-parts":[[2012,1]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Previous studies have established that mycobacterial infections ameliorate allergic inflammation. However, a non\u2010infectious approach that controls allergic responses might represent a safer and more promising strategy. The 60\u201365\u00a0kDa heat shock protein (<jats:styled-content style=\"fixed-case\">Hsp<\/jats:styled-content>) family is endowed with anti\u2010inflammatory properties, but it is still unclear whether and how single mycobacterial <jats:styled-content style=\"fixed-case\">Hsp<\/jats:styled-content> control allergic disorders.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>Therefore, in this study we determined whether the administration of <jats:styled-content style=\"fixed-case\"><jats:italic>M<\/jats:italic><\/jats:styled-content><jats:italic>ycobacterial leprae<\/jats:italic> <jats:styled-content style=\"fixed-case\">Hsp<\/jats:styled-content>65 expressed by recombinant a <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> plasmid could attenuate a previously established allergic response.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We used an experimental model of airway allergic inflammation to test the effects of immunotherapy with <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> encoding <jats:styled-content style=\"fixed-case\">Hsp<\/jats:styled-content>65. Allergic mice, previously sensitized and challenged with ovalbumin, were treated with tree intramuscular doses of recombinant <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> encoding <jats:styled-content style=\"fixed-case\">Hsp<\/jats:styled-content>65. After treatment, mice received a second allergen challenge and the allergic response was measured.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We found that immunotherapy attenuated eosinophilia, pulmonary inflammation, Th2 cytokine and mucus production. Moreover, we showed that the inhibition of allergic response is dependent on <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201010 production. Both <jats:styled-content style=\"fixed-case\">Hsp<\/jats:styled-content>65 and allergen\u2010specific <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201010\u2010producing cells contributed to this effect. Cells transferred from <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>\u2010immunized mice to allergic mice migrated to allergic sites and down\u2010modulated the Th2 response.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and Clinical Relevance<\/jats:title><jats:p>Our findings clearly show that immunotherapy with <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> encoding <jats:styled-content style=\"fixed-case\">Hsp<\/jats:styled-content>65 can attenuate an established Th2 allergic inflammation through an <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201010\u2010dependent mechanism; moreover, the migration of allergen\u2010 and <jats:styled-content style=\"fixed-case\">Hsp<\/jats:styled-content>65\u2010specific cells to the allergic sites exerts a fundamental role. This work represents a novel contribution to the understanding of immune regulation by <jats:styled-content style=\"fixed-case\">Hsp<\/jats:styled-content>65 in allergic diseases.<\/jats:p><\/jats:sec>","DOI":"10.1111\/j.1365-2222.2011.03845.x","type":"journal-article","created":{"date-parts":[[2011,9,1]],"date-time":"2011-09-01T18:31:27Z","timestamp":1314901887000},"page":"131-143","source":"Crossref","is-referenced-by-count":17,"title":["Recombinant <scp>DNA<\/scp> immunotherapy ameliorate established airway allergy in a <scp>IL<\/scp>\u201010 dependent pathway"],"prefix":"10.1111","volume":"42","author":[{"given":"D. M.","family":"Fonseca","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Immunology School of Medicine of Ribeir\u00e3o Preto University of S\u00e3o Paulo Riber\u00e3o Preto Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P. F.","family":"Wowk","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Immunology School of Medicine of Ribeir\u00e3o Preto University of S\u00e3o Paulo Riber\u00e3o Preto Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. O.","family":"Paula","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Immunology School of Medicine of Ribeir\u00e3o Preto University of S\u00e3o Paulo Riber\u00e3o Preto Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L. W.","family":"Campos","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Immunology School of Medicine of Ribeir\u00e3o Preto University of S\u00e3o Paulo Riber\u00e3o Preto Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. F.","family":"Gembre","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Immunology School of Medicine of Ribeir\u00e3o Preto University of S\u00e3o Paulo Riber\u00e3o Preto Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W. M.","family":"Turato","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Immunology School of Medicine of Ribeir\u00e3o Preto University of S\u00e3o Paulo Riber\u00e3o Preto Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. G.","family":"Ramos","sequence":"additional","affiliation":[{"name":"Department of Pathology School of Medicine of Ribeir\u00e3o Preto University of S\u00e3o Paulo Riber\u00e3o Preto Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Dias\u2010Baruffi","sequence":"additional","affiliation":[{"name":"Department of Clinical, Toxicological, Bromatological Analyses School of Pharmaceutical Sciences of Ribeir\u00e3o Preto University of S\u00e3o Paulo Riber\u00e3o Preto Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Barboza","sequence":"additional","affiliation":[{"name":"Department of Immunology Institute of Biomedical Sciences University of S\u00e3o Paulo Riber\u00e3o Preto Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Gomes","sequence":"additional","affiliation":[{"name":"Department of Immunology Institute of Biomedical Sciences University of S\u00e3o Paulo Riber\u00e3o Preto Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. L.","family":"Silva","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Immunology School of Medicine of Ribeir\u00e3o Preto University of S\u00e3o Paulo Riber\u00e3o Preto Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Russo","sequence":"additional","affiliation":[{"name":"Department of Immunology Institute of Biomedical Sciences University of S\u00e3o Paulo Riber\u00e3o Preto Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V. L. D.","family":"Bonato","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Immunology School of Medicine of Ribeir\u00e3o Preto University of S\u00e3o Paulo Riber\u00e3o Preto Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,9]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2009.10.064"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11882-009-0081-7"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-immunol-030409-101218"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.med.60.041807.123524"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1398-9995.2003.00335.x"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1398-9995.2003.00182.x"},{"key":"e_1_2_6_8_1","first-page":"195","article-title":"DNA vaccines: an evolving approach to the treatment of allergic disorders","volume":"26","author":"Broide DH","year":"2005","journal-title":"Allergy Asthma Proc"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.299.6710.1259"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00281-003-0148-9"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.0109005"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00430-007-0055-0"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2010.11.001"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri1593"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/1476-8518-7-4"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1089\/hum.2005.16.1338"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.18.1.927"},{"key":"e_1_2_6_18_1","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1128\/IAI.66.1.169-175.1998","article-title":"Identification and characterization of protective T cells in hsp65 DNA\u2010vaccinated and Mycobacterium tuberculosis\u2010infected mice","volume":"66","author":"Bonato VL","year":"1998","journal-title":"Infect Immun"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1517\/14712598.8.9.1255"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/icb.2011.9"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2567.2007.02597.x"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00580-09"},{"key":"e_1_2_6_23_1","first-page":"689","article-title":"Histologic analysis of an immune response in the lung parenchyma of mice. Angiopathy accompanies inflammatory cell influx","volume":"137","author":"Curtis JL","year":"1990","journal-title":"Am J Pathol"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.2008.03036.x"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.187.4.561"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm0602-625"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.2010.04148.x"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0901379"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2010.11.030"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11882-009-0085-3"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.177.11.7634"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0902829"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.177.2.573"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20051680"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.190.9.1309"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.169.9.5300"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.2004.1931.x"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/cgt.2009.9"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1177\/1721727X0900700204"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.icb.7100044"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20030152"},{"key":"e_1_2_6_42_1","doi-asserted-by":"crossref","first-page":"1347","DOI":"10.1038\/ni.1677","article-title":"IL\u20104 inhibits TGF\u2010beta\u2010induced Foxp3\u00a0+\u00a0 T cells and, together with TGF\u2010beta, generates IL\u20109\u00a0+\u00a0 IL\u201010\u00a0+\u00a0 Foxp3(\u2010) effector T cells","volume":"9","author":"Dardalhon V","year":"2008","journal-title":"Nat Immunol"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1590\/S1806-37132008001100002"}],"container-title":["Clinical &amp; Experimental Allergy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1365-2222.2011.03845.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1365-2222.2011.03845.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,23]],"date-time":"2023-09-23T14:41:30Z","timestamp":1695480090000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1365-2222.2011.03845.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,9]]},"references-count":42,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2012,1]]}},"alternative-id":["10.1111\/j.1365-2222.2011.03845.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1365-2222.2011.03845.x","archive":["Portico"],"relation":{},"ISSN":["0954-7894","1365-2222"],"issn-type":[{"value":"0954-7894","type":"print"},{"value":"1365-2222","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,9]]}}}