{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T22:47:16Z","timestamp":1778885236104,"version":"3.51.4"},"reference-count":38,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2011,9,20]],"date-time":"2011-09-20T00:00:00Z","timestamp":1316476800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Experimental Allergy"],"published-print":{"date-parts":[[2011,12]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Glucocorticoids are the mainstream drugs used in the treatment and control of inflammatory diseases such as asthma. Annexin\u20101 (<jats:styled-content style=\"fixed-case\">ANXA<\/jats:styled-content>1) is an anti\u2010inflammatory protein which has been described as an endogenous protein responsible for some anti\u2010inflammatory glucocorticoid effects. Previous studies have identified its importance in other immune diseases such as rheumatoid arthritis and cystic fibrosis. <jats:styled-content style=\"fixed-case\">ANXA<\/jats:styled-content>1\u2010deficient (<jats:sup>\u2212\/\u2212<\/jats:sup>) mice are Th2 biased, and <jats:styled-content style=\"fixed-case\">ANXA<\/jats:styled-content>1 <jats:styled-content style=\"fixed-case\">N<\/jats:styled-content>\u2010terminus peptide exhibits anti\u2010inflammatory activity in a rat model of pulmonary inflammation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">ANXA<\/jats:styled-content>1 protein is found in bronchoalveolar lavage fluid from asthmatics. However, the function of <jats:styled-content style=\"fixed-case\">ANXA<\/jats:styled-content>1 in the pathological development of allergy or asthma is unclear. Thus, in this study we intended to examine the effect of <jats:styled-content style=\"fixed-case\">ANXA<\/jats:styled-content>1 deficiency on allergen\u2010specific antibody responses and airway responses to methacholine (Mch).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">ANXA<\/jats:styled-content>1<jats:sup>\u2212\/\u2212<\/jats:sup> mice were sensitized with ovalbumin (<jats:styled-content style=\"fixed-case\">OVA<\/jats:styled-content>) and challenged with aerosolized <jats:styled-content style=\"fixed-case\">OVA<\/jats:styled-content>. Airway resistance, lung compliance and enhanced pause (<jats:styled-content style=\"fixed-case\">PenH<\/jats:styled-content>) were measured in na\u00efve, sensitized and saline or allergen\u2010challenged wild\u2010type (<jats:styled-content style=\"fixed-case\">WT<\/jats:styled-content>) and <jats:styled-content style=\"fixed-case\">ANXA<\/jats:styled-content>1<jats:sup>\u2212\/\u2212<\/jats:sup> mice. Total and allergen\u2010specific antibodies were measured in the serum.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We show that allergen\u2010specific and total <jats:styled-content style=\"fixed-case\">IgE<\/jats:styled-content>, <jats:styled-content style=\"fixed-case\">IgG2a<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">IgG2b<\/jats:styled-content> levels were significantly higher in <jats:styled-content style=\"fixed-case\">ANXA<\/jats:styled-content>1<jats:sup>\u2212\/\u2212<\/jats:sup> mice. Furthermore, na\u00efve <jats:styled-content style=\"fixed-case\">ANXA<\/jats:styled-content>1<jats:sup>\u2212\/\u2212<\/jats:sup> mice displayed higher airway hypersensitivity to inhaled Mch, and significant differences were also observed in allergen\u2010sensitized and allergen\u2010challenged <jats:styled-content style=\"fixed-case\">ANXA<\/jats:styled-content>1<jats:sup>\u2212\/\u2212<\/jats:sup> mice compared with <jats:styled-content style=\"fixed-case\">WT<\/jats:styled-content> mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>In conclusion, <jats:styled-content style=\"fixed-case\">ANXA<\/jats:styled-content>1<jats:sup>\u2212\/\u2212<\/jats:sup> mice possess multiple features characteristic to allergic asthma, such as airway hyperresponsiveness and enhanced antibody responses, suggesting that <jats:styled-content style=\"fixed-case\">ANXA<\/jats:styled-content>1 plays a critical regulatory role in the development of asthma.<\/jats:p><\/jats:sec><jats:sec><jats:title>Clinical Relevance<\/jats:title><jats:p>We postulate that <jats:styled-content style=\"fixed-case\">ANXA<\/jats:styled-content>1 is an important regulatory factor in the development of allergic disease and dysregulation of its expression can lead to pathological changes which may affect disease progression.<\/jats:p><\/jats:sec>","DOI":"10.1111\/j.1365-2222.2011.03855.x","type":"journal-article","created":{"date-parts":[[2011,9,21]],"date-time":"2011-09-21T02:42:34Z","timestamp":1316572954000},"page":"1793-1803","source":"Crossref","is-referenced-by-count":44,"title":["Annexin\u20101\u2010deficient mice exhibit spontaneous airway hyperresponsiveness and exacerbated allergen\u2010specific antibody responses in a mouse model of asthma"],"prefix":"10.1111","volume":"41","author":[{"given":"F. 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