{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,5]],"date-time":"2026-02-05T12:58:01Z","timestamp":1770296281391,"version":"3.49.0"},"reference-count":30,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2011,9,20]],"date-time":"2011-09-20T00:00:00Z","timestamp":1316476800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Experimental Allergy"],"published-print":{"date-parts":[[2012,1]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Atopic keratoconjunctivitis (<jats:styled-content style=\"fixed-case\">AKC<\/jats:styled-content>) is a chronic eye disease with periods of exacerbations. Many patients experience no obvious seasonal variation, although a majority of patients are allergic to common airborne allergens.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>To investigate the allergic reaction, to conjunctival provocation with airborne allergens, in patients with <jats:styled-content style=\"fixed-case\">AKC<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Eleven patients with <jats:styled-content style=\"fixed-case\">AKC<\/jats:styled-content> and birch and\/or grass pollen allergy participated in the study, which was performed outside the pollen season. Five patients with seasonal allergic conjunctivitis (<jats:styled-content style=\"fixed-case\">SAC<\/jats:styled-content>) and five healthy subjects were included for validation purposes. The challenge was performed in one eye with the allergen, to which the patient was reactive, and with dilution buffer in the other eye. Signs and symptoms from both eyes were graded at baseline and at 10\u00a0min, 8 and 48\u00a0h after provocation. Tear fluid was collected from both eyes for cytokine analyses at baseline and at 8 and 48\u00a0h.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A significant change in clinical symptoms and signs, (redness and chemosis) was evident 10\u00a0min after provocation compared with baseline (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.005) and compared with the unprovoked eye (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.005) in <jats:styled-content style=\"fixed-case\">AKC<\/jats:styled-content> subjects. These parameters were normalized after 8 and 48\u00a0h. A significant increase for <jats:styled-content style=\"fixed-case\">IFN<\/jats:styled-content>\u2010\u03b3 (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.021) and <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20106 (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.015), and a near significant increase for <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201010 (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.066) were seen in the tear fluid of the challenged eye at 48\u00a0h after provocation vs. baseline and vs. the control eye for <jats:styled-content style=\"fixed-case\">IFN<\/jats:styled-content>\u2010\u03b3 (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.005), <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20106 (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.028) and <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201010 (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.008) in <jats:styled-content style=\"fixed-case\">AKC<\/jats:styled-content> subjects.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion and Clinical Relevance<\/jats:title><jats:p>In this single dose allergen provocation study, <jats:styled-content style=\"fixed-case\">AKC<\/jats:styled-content> patients responded with a typical <jats:styled-content style=\"fixed-case\">IgE<\/jats:styled-content>\u2010mediated allergic reaction. An increase in cytokines at 48\u00a0h after the challenge was demonstrated and might, with further studies, give us a better understanding of the nature of inflammation in <jats:styled-content style=\"fixed-case\">AKC<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/j.1365-2222.2011.03858.x","type":"journal-article","created":{"date-parts":[[2011,9,21]],"date-time":"2011-09-21T02:43:33Z","timestamp":1316573013000},"page":"58-65","source":"Crossref","is-referenced-by-count":15,"title":["Conjunctival provocation with airborne allergen in patients with atopic keratoconjunctivitis"],"prefix":"10.1111","volume":"42","author":[{"given":"E.","family":"Nivenius","sequence":"first","affiliation":[{"name":"Division of Ophthalmology and Vision Department of Clinical Neuroscience Karolinska Institutet St. Erik's Eye Hospital Stockholm Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I.","family":"Van der Ploeg","sequence":"additional","affiliation":[{"name":"Division of Ophthalmology and Vision Department of Clinical Neuroscience Karolinska Institutet St. Erik's Eye Hospital Stockholm Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guro","family":"Gafvelin","sequence":"additional","affiliation":[{"name":"Clinical Immunology and Allergy Unit Department of Medicine Karolinska Institutet Karolinska University Hospital Stockholm Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Van Hage","sequence":"additional","affiliation":[{"name":"Clinical Immunology and Allergy Unit Department of Medicine Karolinska Institutet Karolinska University Hospital Stockholm Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P. G.","family":"Montan","sequence":"additional","affiliation":[{"name":"Division of Ophthalmology and Vision Department of Clinical Neuroscience Karolinska Institutet St. Erik's Eye Hospital Stockholm Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,9,20]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0161-6420(91)32322-4"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.2004.1950.x"},{"key":"e_1_2_6_4_1","first-page":"21","article-title":"Ocular complications of atopic dermatitis","volume":"19","author":"Garrity JA","year":"1984","journal-title":"Can J Ophthalmol"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00417-006-0285-7"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.2006.02499.x"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-6749(97)70279-3"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.1984.tb01931.x"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1001\/archopht.1990.01070030090035"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1089\/jop.1998.14.533"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0420.2005.00622.x"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-6749(05)80046-6"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.eye.6702367"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2222.2000.00699.x"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-6749(05)80148-4"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1067\/mai.2000.110930"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.5387\/fms.47.51"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.1996.tb00498.x"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2222.1999.00585.x"},{"key":"e_1_2_6_20_1","first-page":"392","article-title":"The immunomodulatory effect of topical cyclosporin A in atopic keratoconjunctivitis","volume":"40","author":"Hingorani M","year":"1999","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-6749(96)80161-8"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-6749(96)70197-5"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1067\/mai.2000.110475"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2009.02218.x"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2008.01860.x"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1081-1206(10)62382-4"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10384-007-0461-2"},{"key":"e_1_2_6_28_1","doi-asserted-by":"crossref","first-page":"2688","DOI":"10.4049\/jimmunol.155.5.2688","article-title":"IL\u201013 expression at the sites of allergen challenge in patients with asthma","volume":"155","author":"Huang SK","year":"1995","journal-title":"J Immunol"},{"key":"e_1_2_6_29_1","first-page":"2465","article-title":"Evaluation of lipid oxidative stress status and inflammation in atopic ocular surface disease","volume":"16","author":"Wakamatsu TH","year":"2010","journal-title":"Mol Vis"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1755-3768.2010.01978.x"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00417-005-0127-z"}],"container-title":["Clinical &amp; Experimental Allergy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1365-2222.2011.03858.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1365-2222.2011.03858.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,23]],"date-time":"2023-09-23T14:40:36Z","timestamp":1695480036000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1365-2222.2011.03858.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,9,20]]},"references-count":30,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2012,1]]}},"alternative-id":["10.1111\/j.1365-2222.2011.03858.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1365-2222.2011.03858.x","archive":["Portico"],"relation":{},"ISSN":["0954-7894","1365-2222"],"issn-type":[{"value":"0954-7894","type":"print"},{"value":"1365-2222","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,9,20]]}}}