{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T20:32:16Z","timestamp":1783110736278,"version":"3.54.6"},"reference-count":45,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2011,12,14]],"date-time":"2011-12-14T00:00:00Z","timestamp":1323820800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Experimental Allergy"],"published-print":{"date-parts":[[2012,3]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Egg allergy is a very common finding in early childhood. Detecting hen's egg (<jats:styled-content style=\"fixed-case\">HE<\/jats:styled-content>) allergy outgrowing and reintroduction of food containing egg is a task for the allergist.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>We sought to evaluate the suitability of boiled egg food challenge compared with <jats:styled-content style=\"fixed-case\">IgE<\/jats:styled-content> to allergenic molecules from <jats:styled-content style=\"fixed-case\">HE<\/jats:styled-content> white using a microarray system.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method<\/jats:title><jats:p>Sixty\u2010eight children referring to our centre by the family paediatricians for a suspected egg allergy were enrolled. Patients underwent double\u2010blind, placebo\u2010controlled food challenge with boiled and raw eggs. Challenge outcomes were compared with skin tests performed using egg white and yolk commercial extracts, to prick\u2010prick test with boiled and raw egg white and yolk, total <jats:styled-content style=\"fixed-case\">IgE<\/jats:styled-content>, egg white specific <jats:styled-content style=\"fixed-case\">IgE<\/jats:styled-content> detected using Immuno<jats:styled-content style=\"fixed-case\">CAP<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">IgE<\/jats:styled-content> to egg allergens available on the immunosolid phase allergen chip (<jats:styled-content style=\"fixed-case\">ISAC<\/jats:styled-content>) 103 microarray.<\/jats:p><\/jats:sec><jats:sec><jats:title>Result<\/jats:title><jats:p>Nineteen subjects (28%) were reactive to both raw and boiled egg, 14 (20.5%) to raw egg only and 35 (51.4%) tolerated both boiled and raw egg. Efficiency analysis was carried out using both raw and boiled egg challenges as gold standard. Forty four of 47 <jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>al d 1 negative patients tolerated boiled egg (94%). Conversely, 20 of 21 <jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>al d 1 positive patients reacted to raw egg (95%). None of the other tests was able to discriminate patients\u2019 response to <jats:styled-content style=\"fixed-case\">HE<\/jats:styled-content> challenge. Furthermore, <jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>al d 1 positivity seems to lead to broader environmental allergen <jats:styled-content style=\"fixed-case\">IgE<\/jats:styled-content> sensitization.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion and Clinical Relevance<\/jats:title><jats:p>The <jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>al d 1 <jats:styled-content style=\"fixed-case\">IgE<\/jats:styled-content> reactivity appears to be a very good predictor of <jats:styled-content style=\"fixed-case\">HE<\/jats:styled-content> clinical allergy. <jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>al d 1 positive children have a high frequency of <jats:styled-content style=\"fixed-case\">HE<\/jats:styled-content> allergy, whereas <jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>al d 1 negative children have a high frequency of tolerance to boiled egg. Multiple specific <jats:styled-content style=\"fixed-case\">IgE<\/jats:styled-content> detection by means of <jats:styled-content style=\"fixed-case\">ISAC<\/jats:styled-content> improves the diagnostic approach in <jats:styled-content style=\"fixed-case\">HE<\/jats:styled-content> allergic children, disclosing other food and inhalant allergic sensitizations, anyhow requiring a comprehensive clinical evaluation.<\/jats:p><\/jats:sec>","DOI":"10.1111\/j.1365-2222.2011.03915.x","type":"journal-article","created":{"date-parts":[[2011,12,14]],"date-time":"2011-12-14T12:01:03Z","timestamp":1323864063000},"page":"441-450","source":"Crossref","is-referenced-by-count":87,"title":["Ovomucoid (<scp>G<\/scp>al d 1) specific <scp>IgE<\/scp> detected by microarray system predict tolerability to boiled hen's egg and an increased risk to progress to multiple environmental allergen sensitisation"],"prefix":"10.1111","volume":"42","author":[{"given":"C.","family":"Alessandri","sequence":"first","affiliation":[{"name":"Center for Molecular Allergology IDI\u2010IRCCS Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Zennaro","sequence":"additional","affiliation":[{"name":"Center for Molecular Allergology IDI\u2010IRCCS Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Scala","sequence":"additional","affiliation":[{"name":"Center for Molecular Allergology IDI\u2010IRCCS Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Ferrara","sequence":"additional","affiliation":[{"name":"Center for Molecular Allergology IDI\u2010IRCCS Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. 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