{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T17:37:16Z","timestamp":1761154636118},"reference-count":37,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2004,6,8]],"date-time":"2004-06-08T00:00:00Z","timestamp":1086652800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2004,6,8]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>Paracoccidioidomycosis is a systemic mycosis endemic in Latin America, with a high prevalence in Brazil, Argentina, Colombia and Venezuela. The aetiological agent of disease is the thermal dimorphic fungus, Paracoccidioides brasiliensis. A glycoprotein of 43\u2003kD (gp43) is the major antigen of P. brasiliensis. Antibodies directed to this antigen are detected in the sera of all patients with paracoccidioidomycosis (PCM). Recently, it has been shown that mice immunized with anti-gp43 monoclonal antibodies (MAbs) (Ab1), induce the idiotypic cascade in the gp43 system, which produced both, anti-Id antibodies (Ab2) and anti-anti-Id antibodies (Ab3). To further characterize the idiotypic cascade modulation in mice immunized with anti-gp43 MAb 17c, hybridomas were produced. Ab2 MAbs named 7.B12 inhibited (&amp;gt;95%) the binding of gp43 to MAb 17c (Ab1), suggesting that this anti-Id MAb bind to the idiotope, thus fulfilling the internal image criteria. To elucidate whether Ab2 MAb could act as antigen in serological assays, instead of gp43, sera from PCM patients were tested. Using an ELISA test, it was observed that antibodies from patients and not normal serum bound to Ab2. However, the ELISA test using Ab2 bound to the solid phase made possible to serologically monitor the patients after antifungal therapy, showing an equivalent curve when compared with ELISA test employing purified gp43. Our results also showed that, when mice were immunized with Ab2\u03b2 and their cells were exposed to gp43 in vitro, a T cell proliferation response was observed.<\/jats:p>","DOI":"10.1111\/j.1365-2249.2004.02507.x","type":"journal-article","created":{"date-parts":[[2004,6,15]],"date-time":"2004-06-15T19:24:39Z","timestamp":1087327479000},"page":"123-128","source":"Crossref","is-referenced-by-count":9,"title":["B and T cell responses elicited by monoclonal anti-idiotypic antibody (Ab2<i>\u03b2<\/i>) mimicking gp43 from<i>Paracoccidioides brasiliensis<\/i>"],"prefix":"10.1093","volume":"137","author":[{"given":"E B","family":"SOUZA","sequence":"first","affiliation":[{"name":"Departamento de An\u00e1lises Cl\u00ednicas & Toxicol\u00f3gicas, Faculdade de Ci\u00eancias Farmac\u00eauticas, Universidade de S\u00e3o Paulo"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J D","family":"LOPES","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de S\u00e3o Paulo, S\u00e3o Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S R","family":"ALMEIDA","sequence":"additional","affiliation":[{"name":"Departamento de An\u00e1lises Cl\u00ednicas & Toxicol\u00f3gicas, Faculdade de Ci\u00eancias Farmac\u00eauticas, Universidade de S\u00e3o Paulo"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2004,6,8]]},"reference":[{"key":"2022011223190500700_b1","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1080\/02681219380000121","article-title":"Paracoccidioidomycosis and its etiological agent Paracoccidioides brasiliensis","volume":"31","author":"San Blas","year":"1993","journal-title":"J Med Vet Mycol"},{"key":"2022011223190500700_b2","first-page":"57","article-title":"Development of vaccines and their use in the prevention of fungal infections","volume":"36","author":"Dixon","year":"1998","journal-title":"Med Mycol"},{"key":"2022011223190500700_b3","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1080\/02681218780000021","article-title":"Host-parasite relationship in Paracoccidioidomycosis","volume":"25","author":"Franco","year":"1987","journal-title":"J Med Vet Mycol"},{"key":"2022011223190500700_b4","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1006\/cimm.1998.1388","article-title":"Pattern of immune response to gp43 from P. brasiliensis in susceptible and resistant mice is influenced by Antigen-presenting Cells","volume":"190","author":"Almeida","year":"1998","journal-title":"Cell Immunol"},{"key":"2022011223190500700_b5","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1080\/02681218780000231","article-title":"Experimental murine paracoccidioidomycosis induce by inhalation of","volume":"25","author":"McEween","year":"1987","journal-title":"Conidia J Med Vet Mycol"},{"key":"2022011223190500700_b6","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1016\/0008-8749(76)90220-3","article-title":"In vivo and in vitro evaluation of cell-mediated immunity in patients with Paracoccidioidomycosis","volume":"24","author":"Musatti","year":"1976","journal-title":"Cell Immunol"},{"key":"2022011223190500700_b7","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1128\/CMR.6.2.89","article-title":"Paracoccidioidomycosis: an update","volume":"6","author":"Brummer","year":"1993","journal-title":"Clin Microbiol Rev"},{"key":"2022011223190500700_b8","doi-asserted-by":"crossref","first-page":"617","DOI":"10.1016\/0035-9203(84)90220-7","article-title":"Serology of paracoccidioidomycosis. 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