{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,28]],"date-time":"2025-09-28T15:34:16Z","timestamp":1759073656179},"reference-count":24,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2005,7,28]],"date-time":"2005-07-28T00:00:00Z","timestamp":1122508800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2005,7,28]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>BALB\/c mice immunized with recombinant Trypanosoma cruzi ribosomal P2\u03b2 protein (TcP2\u03b2) develop a strong and specific antibody response against its 13 residue-long C-terminal epitope (peptide R13: EEEDDDMGFGLFD) that has a concomitant \u03b21-adrenergic stimulating activity. However, other animals that undergo similar immunizations seem tolerant to this epitope. To evaluate further the antibody response against the ribosomal P proteins, 25 BALB\/c and 25 Swiss mice were immunized with TcP2\u03b2. From the 50 animals, 31 developed a positive anti-R13 response, whereas 19 were non-responsive. From the 31 anti-R13 positive mice, 25 had anti-R13 antibodies that recognized the discontinuous motif ExDDxGF, and their presence correlated with the recording of supraventricular tachycardia. The other six had anti-R13 antibodies but with a normal electrocardiographic recording. These anti-R13 antibodies recognized the motif DDxGF shared by mammals and T. cruzi and proved to be a true anti-P autoantibody because they were similar to those elicited in Swiss, but not in BALB\/c mice, by immunization with the C-terminal portion of the mouse ribosomal P protein. Our results show that the recognition of the glutamic acid in position 3 of peptide R13 defines the ability of anti-R13 antibodies to react with the motif AESDE of the second extracellular loop of the \u03b21-adrenergic receptor, setting the molecular basis for their pathogenic \u03b21 adrenoceptor stimulating activity.<\/jats:p>","DOI":"10.1111\/j.1365-2249.2005.02885.x","type":"journal-article","created":{"date-parts":[[2005,9,13]],"date-time":"2005-09-13T10:01:14Z","timestamp":1126605674000},"page":"140-147","source":"Crossref","is-referenced-by-count":14,"title":["The \u03b21 adrenergic effects of antibodies against the C-terminal end of the ribosomal P2\u03b2 protein of<i>Trypanosoma cruzi<\/i>associate with a specific pattern of epitope recognition"],"prefix":"10.1093","volume":"142","author":[{"given":"P Lopez","family":"Bergami","sequence":"first","affiliation":[{"name":"Laboratorio de Biolog\u00eda Molecular de la Enfermedad de Chagas, Instituto de Investigaciones en Ingenier\u00eda Gen\u00e9tica y Biolog\u00eda Molecular-Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (INGEBI\/CONICET) and CeGA, Centro de Gen\u00f3mica Aplicada-Universidad de Buenos Aires, Buenos Aires, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K A","family":"G\u00f3mez","sequence":"additional","affiliation":[{"name":"Laboratorio de Biolog\u00eda Molecular de la Enfermedad de Chagas, Instituto de Investigaciones en Ingenier\u00eda Gen\u00e9tica y Biolog\u00eda Molecular-Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (INGEBI\/CONICET) and CeGA, Centro de Gen\u00f3mica Aplicada-Universidad de Buenos Aires, Buenos Aires, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G V","family":"Levy","sequence":"additional","affiliation":[{"name":"Laboratorio de Biolog\u00eda Molecular de la Enfermedad de Chagas, Instituto de Investigaciones en Ingenier\u00eda Gen\u00e9tica y Biolog\u00eda Molecular-Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (INGEBI\/CONICET) and CeGA, Centro de Gen\u00f3mica Aplicada-Universidad de Buenos Aires, Buenos Aires, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V","family":"Grippo","sequence":"additional","affiliation":[{"name":"Laboratorio de Biolog\u00eda Molecular de la Enfermedad de Chagas, Instituto de Investigaciones en Ingenier\u00eda Gen\u00e9tica y Biolog\u00eda Molecular-Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (INGEBI\/CONICET) and CeGA, Centro de Gen\u00f3mica Aplicada-Universidad de Buenos Aires, Buenos Aires, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"Baldi","sequence":"additional","affiliation":[{"name":"Instituto de Biolog\u00eda y Medicina Experimental (IBYME), Buenos Aires, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M 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