{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T05:29:04Z","timestamp":1761974944627},"reference-count":37,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2006,2,16]],"date-time":"2006-02-16T00:00:00Z","timestamp":1140048000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2006,2,16]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Whereas the involvement of Th1- and Th2-type cytokines in contact allergy to nickel (Ni) is well documented, the role of the regulatory cytokine IL-10 is less clear. We therefore investigated the impact of IL-10 on Ni-induced Th1- (IFN-\u03b3) and Th2-type (IL-4 and IL-13) cytokine responses in human peripheral blood mononuclear cells (PBMC). PBMC from 15 blood donors with reactivity to Ni (Ni-PBMC) and 8 control donors devoid of reactivity (control PBMC) were stimulated with Ni and the frequency of cytokine-producing cells and the levels of secreted cytokines were analysed by ELISpot (IL-4, IL-13 and IFN-\u03b3) and ELISA (IL-10, IL-13 and IFN-\u03b3), respectively. The Ni-induced response was further assessed in the presence of recombinant IL-10 (rIL-10) or neutralizing antibody to IL-10 and the phenotype of the Ni-specific cytokine-producing cells regulated by IL-10 was determined by cell depletion experiments. Ni induced IL-10 production in Ni-PBMC (mean, (range); 33\u00b71\u2003pg\/ml (0\u201393\u00b74\u2003pg\/ml)) but not control PBMC (2\u00b72\u2003pg\/ml (0\u201314\u00b79\u2003pg\/ml)) (P\u2003=\u20030\u00b7002). Ni also induced significant production of IL-4, IL-13 and IFN-\u03b3 that correlated with the IL-10 response. Addition of rIL-10 down-regulated the Ni-induced production of all cytokines but with a more pronounced effect on IFN-\u03b3. However, neutralization of Ni-induced IL-10 enhanced the levels of IFN-\u03b3 induced by Ni (P\u2003=\u20030\u00b7004) but did not affect the number of IFN-\u03b3-producing cells or the production of other cytokines. Cell depletion experiments suggested that the Ni-specific IFN-\u03b3 (and Th2-type cytokine) producing cells were CD4+ T cells. The impact of IL-10 on Ni-induced IFN-\u03b3 responses by CD4+ T cells suggests that an important role of IL-10 in vivo is to counteract the allergic reactions mediated by Th1-type cytokines.<\/jats:p>","DOI":"10.1111\/j.1365-2249.2006.03018.x","type":"journal-article","created":{"date-parts":[[2006,2,16]],"date-time":"2006-02-16T06:08:32Z","timestamp":1140070112000},"page":"494-502","source":"Crossref","is-referenced-by-count":33,"title":["Nickel-induced IL-10 down-regulates Th1- but not Th2-type cytokine responses to the contact allergen nickel"],"prefix":"10.1093","volume":"143","author":[{"given":"J T","family":"Minang","sequence":"first","affiliation":[{"name":"Department of Immunology, Stockholm University, Stockholm"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I","family":"Arestr\u00f6m","sequence":"additional","affiliation":[{"name":"Mabtech AB, Nacka Strand, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B","family":"Zuber","sequence":"additional","affiliation":[{"name":"Mabtech AB, Nacka Strand, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G","family":"J\u00f6nsson","sequence":"additional","affiliation":[{"name":"Mabtech AB, Nacka Strand, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Troye-Blomberg","sequence":"additional","affiliation":[{"name":"Department of Immunology, Stockholm University, Stockholm"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N","family":"Ahlborg","sequence":"additional","affiliation":[{"name":"Department of Immunology, Stockholm University, Stockholm"},{"name":"Mabtech AB, Nacka Strand, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2006,2,16]]},"reference":[{"key":"2022010522203456300_b1","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1111\/j.1600-0536.1997.tb02466.x","article-title":"Patch test results in schoolchildren. 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