{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,1,23]],"date-time":"2024-01-23T08:58:17Z","timestamp":1706000297406},"reference-count":24,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2006,5,11]],"date-time":"2006-05-11T00:00:00Z","timestamp":1147305600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2006,5,22]]},"abstract":"<jats:title>Summary<\/jats:title>\n               <jats:p>Mucous membrane pemphigoid (MMP) (also known as cicatricial pemphigoid) is a rare autoimmune mucocutaneous blistering disease that affects mucous membranes derived from stratified squamous epithelium and the skin. A subset of MMP affects only the oral cavity and is referred to as the oral pemphigoid (OP). MMP and OP are characterized by subepithelial vesicles on histology and in vivo deposition of immunoglobulins and complement at the basement membrane zone (BMZ) on immunopathology. Previous studies have shown that sera of patients with MMP bind to human integrin \u03b24, while sera of patients with oral pemphigoid bind to the integrin \u03b16 component of the heterodimer. The prognosis in MMP is grave but excellent in OP. In this study we compare the binding of sera from patients with OP from Boston, MA, USA to Naples, Italy, and attempt to identify an epitope to which the anti-integrin \u03b16 human autoantibody binds. Our results indicate that the sera from Boston and Naples are identical in their reactivity. They recognize a fragment I (AA 23\u2013462) and its subfragment IB (AA 217\u2013462) only, in the human integrin \u03b16 molecule. Blocking studies, immunoprecipitation and immunoabsorbtion studies confirm the presence of this single 245 AA region. Antibodies to subfragment IB cause BMZ separation in organ culture using normal human oral mucosa as substrate. This preliminary study indicates that patients on both continents may have similar reactivity and suggests that an intercontinental study group could be established to advance our understanding of the pathogenesis of OP and the biology of anti-\u03b16 integrin autoantibodies.<\/jats:p>","DOI":"10.1111\/j.1365-2249.2006.03103.x","type":"journal-article","created":{"date-parts":[[2006,5,11]],"date-time":"2006-05-11T15:31:25Z","timestamp":1147361485000},"page":"28-35","source":"Crossref","is-referenced-by-count":6,"title":["Comparison of reactivity and epitope recognition between sera from American and Italian patients with oral pemphigoid"],"prefix":"10.1093","volume":"145","author":[{"given":"M","family":"Mignogna","sequence":"first","affiliation":[{"name":"Department of Odontostomatological and Maxillofacial Sciences, University of Naples, School of Medicine, Naples, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"Lanza","sequence":"additional","affiliation":[{"name":"Department of Odontostomatological, Ortodontical and Surgical Disciplines, Dentistry School, 2nd University of Naples, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L","family":"Rossiello","sequence":"additional","affiliation":[{"name":"Department of Dermatology, 2nd University of Naples, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V","family":"Ruocco","sequence":"additional","affiliation":[{"name":"Department of Odontostomatological and Maxillofacial Sciences, University of Naples, School of Medicine, Naples, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A R","family":"Ahmed","sequence":"additional","affiliation":[{"name":"Center for Blistering Diseases, Department of Medicine, New England Baptist Hospital, Boston, MA, USA"},{"name":"Department of Oral Medicine, Infection and Immunity, Harvard School of Dental Medicine, Boston, MA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2006,5,11]]},"reference":[{"key":"2022010506333057200_b1","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1067\/mjd.2000.107248","article-title":"Cicatricial pemphigoid","volume":"43","author":"Fleming","year":"2000","journal-title":"J Am Acad Dermatol"},{"key":"2022010506333057200_b2","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1046\/j.1523-1747.1999.00546.x","article-title":"Structure and function of hemidesmosomes: more than simple adhesion complexes","volume":"112","author":"Borradori","year":"1999","journal-title":"J Invest Dermatol"},{"key":"2022010506333057200_b3","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/S1079-2104(98)90395-X","article-title":"Oral pemphigoid: subset of cicatricial pemphigoid","volume":"85","author":"Mobini","year":"1998","journal-title":"Oral Surg Oral Med Oral Pathol Oral Radiol Endod"},{"key":"2022010506333057200_b4","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/0738-081X(87)90045-9","article-title":"Oral involvement in mucous membrane pemphigoid","volume":"5","author":"Gallagher","year":"1987","journal-title":"Clin Dermatol"},{"key":"2022010506333057200_b5","doi-asserted-by":"crossref","first-page":"1711","DOI":"10.1177\/00220345010800080601","article-title":"Autoantibodies to human \u03b16 integrin patients with oral pemphigoid","volume":"80","author":"Bhol","year":"2001","journal-title":"J Dent Res"},{"key":"2022010506333057200_b6","doi-asserted-by":"crossref","first-page":"829","DOI":"10.1083\/jcb.115.3.829","article-title":"Expression of \u03b21, \u03b23, \u03b24 and \u03b25 integrins by human epidermal keratinocytes and non-differentiating keratinocytes","volume":"115","author":"Adams","year":"1991","journal-title":"J Cell Biol"},{"key":"2022010506333057200_b7","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1016\/S0955-0674(00)00249-0","article-title":"The alpha6 beta4 integrin and epithelial cell migration","volume":"13","author":"Mercurio","year":"2001","journal-title":"Curr Opin Cell Biol"},{"key":"2022010506333057200_b8","doi-asserted-by":"crossref","first-page":"23778","DOI":"10.1074\/jbc.M501365200","article-title":"Desmosome signaling. 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