{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,21]],"date-time":"2026-03-21T20:29:45Z","timestamp":1774124985046,"version":"3.50.1"},"reference-count":19,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2006,5,22]],"date-time":"2006-05-22T00:00:00Z","timestamp":1148256000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2006,5,22]]},"abstract":"<jats:title>Summary<\/jats:title>\n               <jats:p>The objective of this study was to investigate the occurrence of apoptosis of monocytes in an experimental model of multiple trauma and its probable correlation to bacterial translocation. Thirty-two rabbits were applied in three groups: A, controls; B, myotomy of the right femur; and C, myotomy and fracture of the right femur. Blood was sampled for the estimation of endotoxins [lipopolysaccharide (LPS)], tumour necrosis factor (TNF)-\u03b1, malondialdehyde (MDA) and isolation of peripheral blood mononuclear cells (PBMCs). PBMCs, derived after centrifugation over Ficoll, were incubated in flasks and apoptosis of non-adherent lymphocytes and adherent monocytes was estimated after staining for Annexin-V and flow cytometry. TNF-\u03b1 of supernatants of cultured monocytes was also determined. Tissue segments were cultured after death. Median survival of groups A, B and C was\u2003&amp;gt;\u200314,\u2003&amp;gt;\u200314 and 9.00\u2003days, respectively. Apoptosis of lymphocytes in group C was higher than group A at 2, 4 and 48 h and of monocytes in group C higher than group A at 2 and 4 hours. LPS in group C was higher than group A at 2, 4 and 48\u2003h. Apoptosis of lymphocytes and monocytes was correlated positively with serum TNF-\u03b1 and negatively with TNF-\u03b1 of monocyte supernatants. Cultures of organ segments of group A were sterile. Pseudomonas aeruginosa was isolated from liver, lung and spleen in five animals in group B (45.45%) and in six in group C (54.54%). Early apoptosis of blood monocytes supervened after multiple trauma; the phenomenon was accompanied by apoptosis of blood lymphocytes and subsequent bacterial translocation.<\/jats:p>","DOI":"10.1111\/j.1365-2249.2006.03112.x","type":"journal-article","created":{"date-parts":[[2006,5,25]],"date-time":"2006-05-25T09:11:31Z","timestamp":1148548291000},"page":"139-146","source":"Crossref","is-referenced-by-count":13,"title":["Early apoptosis of monocytes contributes to the pathogenesis of systemic inflammatory response and of bacterial translocation in an experimental model of multiple trauma"],"prefix":"10.1093","volume":"145","author":[{"given":"N","family":"Efstathopoulos","sequence":"first","affiliation":[{"name":"2nd Department of Orthopaedics, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T","family":"Tsaganos","sequence":"additional","affiliation":[{"name":"4th Department of Internal Medicine, University of Athens, Medical School, 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