{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T15:47:04Z","timestamp":1759333624276},"reference-count":33,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2006,6,7]],"date-time":"2006-06-07T00:00:00Z","timestamp":1149638400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2006,6,23]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Epidermolysis bullosa acquisita is an autoimmune blistering disease characterized by circulating and skin basement membrane-bound IgG autoantibodies to type VII collagen, a major structural protein of the dermal\u2013epidermal junction. Regulatory T cells (Treg) suppress self antigen-mediated autoimmune responses. To investigate the role of Treg in the the autoimmune response to type VII collagen in a mouse model, a monoclonal antibody against mouse CD25\u2003was used to deplete Treg. A recombinant mouse type VII collagen NC1 domain protein and mouse albumin were used as antigens. SKH1 mice were used as a testing host. Group 1 mice received NC1 immunization and were functionally depleted of Treg; group 2 mice received NC1 immunization and rat isotype control; and group 3 mice received albumin immunization and were functionally depleted of Treg. Results demonstrated that anti-NC1 IgG autoantibodies with high titres, as determined by enzyme-linked immunosorbent assay and Western blotting, developed in all mice immunized with NC1 (groups 1 and 2), but were undetected in group 3 mice. The predominant subclasses of anti-NC1 autoantibodies were IgG1, IgG2a and IgG2b; furthermore, these antibodies carried only the kappa light chain. IgG autoantibodies in the sera of NC1-immunized mice reacted with mouse skin basement membrane in vitro and deposited in skin basement membrane in vivo as detected by indirect and direct immunofluorescence microscopy, respectively. Our data suggest that the development of autoimmunity against type VII collagen in mice is independent of Treg function and the autoimmune response is mediated by both Th1 and Th2 cells. We speculate that the basement membrane deposition of IgG may eventually lead to blister development.<\/jats:p>","DOI":"10.1111\/j.1365-2249.2006.03115.x","type":"journal-article","created":{"date-parts":[[2006,6,7]],"date-time":"2006-06-07T22:08:02Z","timestamp":1149718082000},"page":"322-331","source":"Crossref","is-referenced-by-count":12,"title":["Autoimmunity to type VII collagen in SKH1 mice is independent of regulatory T cells"],"prefix":"10.1093","volume":"145","author":[{"given":"L","family":"Chen","sequence":"first","affiliation":[{"name":"Departments of Dermatology, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J D","family":"Peterson","sequence":"additional","affiliation":[{"name":"Departments of Dermatology, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W-Y","family":"Zheng","sequence":"additional","affiliation":[{"name":"Departments of Dermatology, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S-X","family":"Lin","sequence":"additional","affiliation":[{"name":"Departments of Dermatology, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L S","family":"Chan","sequence":"additional","affiliation":[{"name":"Departments of Dermatology, USA"},{"name":"Microbiology\/Immunology, UIC, Chicago, IL, USA"},{"name":"Medical Service, Jesse Brown VA Medical Center, Chicago, IL, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2006,6,7]]},"reference":[{"key":"2022010506234208100_b1","doi-asserted-by":"crossref","first-page":"1007","DOI":"10.1056\/NEJM198404193101602","article-title":"Identification of the skin basement-membrane autoantigen in epidermolysis bullosa acquisita","volume":"310","author":"Woodley","year":"1984","journal-title":"N Engl J Med"},{"key":"2022010506234208100_b2","doi-asserted-by":"crossref","first-page":"825","DOI":"10.1172\/JCI200524855","article-title":"The pathophysiology of autoimmune blistering diseases","volume":"115","author":"Yancey","year":"2005","journal-title":"J Clin Invest"},{"key":"2022010506234208100_b3","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1111\/1523-1747.ep12285634","article-title":"Development of an ELISA for rapid detection of anti-type VII collagen autoantibodies in epidermolysis bullosa acquisita","volume":"108","author":"Chen","year":"1997","journal-title":"J Invest Dermatol"},{"key":"2022010506234208100_b4","doi-asserted-by":"crossref","first-page":"21649","DOI":"10.1074\/jbc.M100180200","article-title":"The carboxyl terminus of type VII collagen mediates antiparallel dimer formation and constitutes a new antigenic epitope for epidermolysis bullosa acquisita autoantibodies","volume":"276","author":"Chen","year":"2001","journal-title":"J Biol Chem"},{"key":"2022010506234208100_b5","doi-asserted-by":"crossref","first-page":"1831","DOI":"10.1172\/JCI116774","article-title":"Epitope mapping of type VII collagen. 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