{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T05:02:48Z","timestamp":1791262968514,"version":"4.1.0"},"reference-count":44,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2006,7,12]],"date-time":"2006-07-12T00:00:00Z","timestamp":1152662400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2006,7,12]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Accumulating evidence suggests that intestinal epithelial cells (IECs) constitutively express the immunoregulatory cytokine interleukin (IL)-18. IECs also serve as the host cell for the intracellular parasitic protozoan Cryptosporidium parvum. In the present study, C. parvum infection of a human enterocyte cell-line HCT-8 resulted in increased expression of IL-18 mRNA as measured by quantitative reverse transcription\u2013polymerase chain reaction (RT-PCR). IL-18 protein was detected in control uninfected cells and following infection there was increased expression as measured by enzyme-linked immunosorbent assay (ELISA). Gene expression revealed the presence of the IL-18 receptor subunits not only in cell-lines but also in freshly isolated IECs, suggesting that IL-18-mediated signalling events may contribute to epithelial host defence during infection. Recombinant IL-18 inhibited intracellular development of the parasite in HCT-8 and HT-29 cells. Increased expression of bactericidal antibiotic peptides LL-37 and \u03b1-defensin 2 by IL-18 in HCT-8 and HT-29 cells may represent one mode of action by which this pluripotent cytokine aids in limiting the development of intracellular pathogens such as C. parvum in the gastrointestinal tract.<\/jats:p>","DOI":"10.1111\/j.1365-2249.2006.03159.x","type":"journal-article","created":{"date-parts":[[2006,7,12]],"date-time":"2006-07-12T14:49:16Z","timestamp":1152715756000},"page":"555-562","source":"Crossref","is-referenced-by-count":53,"title":["A potential role for interleukin-18 in inhibition of the development of<i>Cryptosporidium parvum<\/i>"],"prefix":"10.1093","volume":"145","author":[{"given":"V","family":"McDonald","sequence":"first","affiliation":[{"name":"Barts and the London School of Medicine and Dentistry, Institute of Cell and Molecular Science, Centre for Gastroenterology, London, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R C G","family":"Pollok","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, St George\u2019s Hospital Medical School, London, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W","family":"Dhaliwal","sequence":"additional","affiliation":[{"name":"Barts and the London School of Medicine and Dentistry, Institute of Cell and Molecular Science, Centre for Gastroenterology, London, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Naik","sequence":"additional","affiliation":[{"name":"Barts and the London School of Medicine and Dentistry, Institute of Cell and Molecular Science, Centre for Gastroenterology, London, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M J G","family":"Farthing","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, St George\u2019s Hospital Medical School, London, 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