{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T11:43:53Z","timestamp":1790855033977,"version":"4.1.0"},"reference-count":20,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2006,8,16]],"date-time":"2006-08-16T00:00:00Z","timestamp":1155686400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2006,8,25]]},"abstract":"<jats:title>Summary<\/jats:title>\n               <jats:p>Serum and plasma are used interchangeably to measure anti-neutrophil cytoplasmic antibodies (ANCA), even though the release of ANCA target antigens during the preparation of serum could affect ANCA assays and cause discrepancies between the results obtained from serum and plasma. To what extent ANCA test results obtained from serum agree and correlate with results from plasma remains unknown. Therefore, a comprehensive comparison was performed using serum and plasma samples which were collected in 175 patients with active Wegener's granulomatosis at enrolment of a recent randomized trial. These paired serum and plasma samples were subjected to parallel ANCA testing by standard indirect immunofluoresence on ethanol-fixed neutrophils, a direct enzyme-linked immunoassay (ELISA) for proteinase 3 (PR3)-ANCA and myeloperoxidase (MPO)-ANCA, and two different capture ELISAs for PR3-ANCA. The concordance of categorical serum and plasma ANCA results was assessed using \u03ba-coefficients. These were &amp;gt;\u20030\u00b78 for all assays, indicating a very good concordance between positive and negative serum and plasma results. Spearman's correlation coefficients for serum and plasma PR3-ANCA values obtained by direct ELISA and both capture ELISAs were \u2265\u20030\u00b795 (P\u2003&amp;lt;\u20030\u00b70001). Our study shows that serum and plasma samples can be used interchangeably for measuring ANCA.<\/jats:p>","DOI":"10.1111\/j.1365-2249.2006.03179.x","type":"journal-article","created":{"date-parts":[[2006,8,16]],"date-time":"2006-08-16T13:15:04Z","timestamp":1155734104000},"page":"15-20","source":"Crossref","is-referenced-by-count":16,"title":["Agreement of anti-neutrophil cytoplasmic antibody measurements obtained from serum and plasma"],"prefix":"10.1093","volume":"146","author":[{"given":"A S","family":"Lee","sequence":"first","affiliation":[{"name":"Thoracic Disease Research Unit, Division of Pulmonary and Critical Care Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J D","family":"Finkielman","sequence":"additional","affiliation":[{"name":"Thoracic Disease Research Unit, Division of Pulmonary and Critical Care Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T","family":"Peikert","sequence":"additional","affiliation":[{"name":"Thoracic Disease Research Unit, Division of Pulmonary and Critical Care Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A M","family":"Hummel","sequence":"additional","affiliation":[{"name":"Thoracic Disease Research Unit, Division of Pulmonary and Critical Care Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M A","family":"Viss","sequence":"additional","affiliation":[{"name":"Thoracic Disease Research Unit, Division of Pulmonary and Critical Care Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G L","family":"Jacob","sequence":"additional","affiliation":[{"name":"Antibody Immunology Laboratory, Division of Clinical Biochemistry and Immunology, Mayo Clinic and Foundation, Rochester, MN 55905, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H A","family":"Homburger","sequence":"additional","affiliation":[{"name":"Antibody Immunology Laboratory, Division of Clinical Biochemistry and Immunology, Mayo Clinic and Foundation, Rochester, MN 55905, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"U","family":"Specks","sequence":"additional","affiliation":[{"name":"Thoracic Disease Research Unit, Division of Pulmonary and Critical Care Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"Wegener\u2019s Granulomatosis Etanercept Trial Research Group","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2006,8,16]]},"reference":[{"key":"2022010503195145000_b1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/0022-1759(96)00111-1","article-title":"Development and standardization of solid phase assays for the detection of anti-neutrophil cytoplasmic antibodies (ANCA). 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