{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T00:36:10Z","timestamp":1784939770118,"version":"3.55.0"},"reference-count":29,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2006,9,27]],"date-time":"2006-09-27T00:00:00Z","timestamp":1159315200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2006,9,27]]},"abstract":"<jats:title>Summary<\/jats:title>\n               <jats:p>Canine visceral leishmaniasis (CVL) manifests itself as a broad clinical spectrum ranging from asymptomatic infection to patent severe disease. Despite relevant findings suggesting changes on lymphocytes subsets regarding the CVL clinical forms, it still remains to be elucidated whether a distinct phenotypic profile would be correlated with degree of tissue parasite density. Herein, we have assessed the correlation between the clinical status as well as the impact of bone marrow parasite density on the phenotypic profile of peripheral blood leucocytes in 40\u2003Brazilian dogs naturally infected by Leishmania chagasi. Our major findings describe the lower frequency of B cells and monocytes as the most important markers of severe CVL. Our main statistically significant findings reveal that the CD8+\u2003T cell subset reflects most accurately both the clinical status and the overall bone marrow parasite density, as increased levels of CD8+ lymphocytes appeared as the major phenotypic feature of asymptomatic disease and dogs bearing a low parasite load. Moreover, enhanced major histocompatibility complex (MHC)-II density as well as a higher CD45RB\/CD45RA expression index seems to represent a key element to control disease morbidity. The association between clinical status, bone marrow parasitism and CD8+\u2003T cells re-emphasizes the role of the T cell-mediated immune response in the resistance mechanisms during ongoing CVL. Higher levels of circulating T lymphocytes (both CD4+\u2003and CD8+\u2003T cells) and lower MHC-II expression by peripheral blood lymphocytes seem to be the key for the effective immunological response, a hallmark of asymptomatic CVL.<\/jats:p>","DOI":"10.1111\/j.1365-2249.2006.03206.x","type":"journal-article","created":{"date-parts":[[2006,9,27]],"date-time":"2006-09-27T09:42:55Z","timestamp":1159350175000},"page":"303-311","source":"Crossref","is-referenced-by-count":76,"title":["Phenotypic features of circulating leucocytes as immunological markers for clinical status and bone marrow parasite density in dogs naturally infected by <i>Leishmania chagasi<\/i>"],"prefix":"10.1093","volume":"146","author":[{"given":"A B","family":"Reis","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Imunopatologia, N\u00facleo de Pesquisas em Ci\u00eancias Biol\u00f3gicas\/NUPEB, Instituto de Ci\u00eancias Exatas e Biol\u00f3gicas, Departamento de An\u00e1lises Cl\u00ednicas\/Escola de Farm\u00e1cia, Universidade Federal de Ouro Preto, Ouro Preto, Minas Gerais, Brazil"},{"name":"Laborat\u00f3rio de Imunologia Celular e Molecular, Centro de Pesquisas Ren\u00e9 Rachou, Funda\u00e7\u00e3o Oswaldo Cruz, Belo Horizonte, Minas Gerais, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A","family":"Teixeira-Carvalho","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Imunopatologia, N\u00facleo de Pesquisas em Ci\u00eancias Biol\u00f3gicas\/NUPEB, Instituto de Ci\u00eancias Exatas e Biol\u00f3gicas, Departamento de An\u00e1lises Cl\u00ednicas\/Escola de Farm\u00e1cia, Universidade Federal de Ouro Preto, Ouro Preto, Minas Gerais, Brazil"},{"name":"Laborat\u00f3rio de Imunologia Celular e Molecular, Centro de Pesquisas Ren\u00e9 Rachou, Funda\u00e7\u00e3o Oswaldo Cruz, Belo Horizonte, Minas Gerais, Brazil"},{"name":"Laborat\u00f3rio de Doen\u00e7a de Chagas, Centro de Pesquisas Ren\u00e9 Rachou, Funda\u00e7\u00e3o Oswaldo Cruz, Belo Horizonte, Minas Gerais, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R C","family":"Giunchetti","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Imunopatologia, N\u00facleo de Pesquisas em Ci\u00eancias Biol\u00f3gicas\/NUPEB, Instituto de Ci\u00eancias Exatas e Biol\u00f3gicas, Departamento de An\u00e1lises Cl\u00ednicas\/Escola de Farm\u00e1cia, Universidade Federal de Ouro Preto, Ouro Preto, Minas Gerais, Brazil"},{"name":"Laborat\u00f3rio de Imunologia Celular e Molecular, Centro de Pesquisas Ren\u00e9 Rachou, Funda\u00e7\u00e3o Oswaldo Cruz, Belo Horizonte, Minas Gerais, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L L","family":"Guerra","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Imunologia Celular e Molecular, Centro de Pesquisas Ren\u00e9 Rachou, Funda\u00e7\u00e3o Oswaldo Cruz, Belo Horizonte, Minas Gerais, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M G","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Hematologia Cl\u00ednica, Faculdade de Farm\u00e1cia, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W","family":"Mayrink","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Leishmanioses, Departamento de Parasitologia, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"O","family":"Genaro","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Imunologia Celular e Molecular, Centro de Pesquisas Ren\u00e9 Rachou, Funda\u00e7\u00e3o Oswaldo Cruz, Belo Horizonte, Minas Gerais, Brazil"},{"name":"Laborat\u00f3rio de Leishmanioses, Departamento de Parasitologia, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R","family":"Corr\u00eaa-Oliveira","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Imunologia Celular e Molecular, Centro de Pesquisas Ren\u00e9 Rachou, Funda\u00e7\u00e3o Oswaldo Cruz, Belo Horizonte, Minas Gerais, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"O A","family":"Martins-Filho","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Doen\u00e7a de Chagas, Centro de Pesquisas Ren\u00e9 Rachou, Funda\u00e7\u00e3o Oswaldo Cruz, Belo Horizonte, Minas Gerais, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2006,9,27]]},"reference":[{"key":"2022010503194526200_b1","first-page":"1","article-title":"Evolution, classification and geographical distribution","volume-title":"The leishmaniasis in biology and 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