{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T21:14:33Z","timestamp":1760908473646},"reference-count":52,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2007,1,11]],"date-time":"2007-01-11T00:00:00Z","timestamp":1168473600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2007,1,15]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Both genetic and environmental factors contribute to autoimmune disease development. Previously, we evaluated genetic factors in a humanized mouse model of Hashimoto's thyroiditis (HT) by immunizing human leucocyte antigen DR3 (HLA-DR3) and HLA-DQ8 transgenic class II-knock-out non-obese diabetic (NOD) mice. DR3+ mice were susceptible to experimental autoimmune thyroiditis (EAT) induction by both mouse thyroglobulin (mTg) and human (h) Tg, while DQ8+ mice were weakly susceptible only to hTg. As one environmental factor associated with HT and tested in non-transgenic models is increased sodium iodide (NaI) intake, we examined the susceptibility of DR3+ and\/or DQ8+ mice to NaI-induced disease. Mice were treated for 8\u2003weeks with NaI in the drinking water. At 0\u00b705% NaI, 23% of DR3+, 0% of DQ8+ and 20% of DR3+DQ8+ mice had thyroid destruction. No spleen cell proliferation to mTg was observed. Most mice had undetectable anti-mTg antibodies, but those with low antibody levels usually had thyroiditis. At 0\u00b73% NaI, a higher percentage of DR3+ and DR3+DQ8+ mice developed destructive thyroiditis, but it was not statistically significant. However, when DR3+ mice had been depleted of CD4+CD25+ regulatory T cells prior to NaI treatment, destructive thyroiditis (68%) and serum anti-mTg antibodies were exacerbated further. The presence of DQ8 molecules does not alter the susceptibility of DR3+DQ8+ mice to NaI-induced thyroiditis, similar to earlier findings with mTg-induced EAT. Susceptibility of DR3+ mice to NaI-induced EAT, in both the presence and absence of regulatory T cells, demonstrates the usefulness of HLA class II transgenic mice in evaluating the roles of environmental factors and immune dysregulation in autoimmune thyroid disease.<\/jats:p>","DOI":"10.1111\/j.1365-2249.2006.03303.x","type":"journal-article","created":{"date-parts":[[2008,10,13]],"date-time":"2008-10-13T18:50:13Z","timestamp":1223923813000},"page":"547-554","source":"Crossref","is-referenced-by-count":27,"title":["Depletion of CD4+CD25+ regulatory T cells exacerbates sodium iodide-induced experimental autoimmune thyroiditis in human leucocyte antigen DR3 (DRB1*0301) transgenic class II-knock-out non-obese diabetic mice"],"prefix":"10.1093","volume":"147","author":[{"given":"J C","family":"Flynn","sequence":"first","affiliation":[{"name":"Department of Immunology and Microbiology, Wayne State University School of Medicine, Detroit, MI, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C","family":"Meroueh","sequence":"additional","affiliation":[{"name":"Department of Pathology, St. John Hospital and Medical Center, Detroit, MI, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D P","family":"Snower","sequence":"additional","affiliation":[{"name":"Department of Pathology, St. John Hospital and Medical Center, Detroit, MI, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C S","family":"David","sequence":"additional","affiliation":[{"name":"Department of Immunology, Mayo Clinic College of Medicine, Rochester, MN, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y M","family":"Kong","sequence":"additional","affiliation":[{"name":"Department of Immunology and Microbiology, Wayne State University School of Medicine, Detroit, MI, 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