{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T13:08:05Z","timestamp":1791119285004,"version":"4.1.0"},"reference-count":33,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2007,4,11]],"date-time":"2007-04-11T00:00:00Z","timestamp":1176249600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2007,4,25]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>The proliferation of V\u03b41+\u2003\u03b3\u03b4\u2003T lymphocytes has been described in various infections including human immunodeficiency virus (HIV), cytomegalovirus (CMV) and malaria. However, the antigen specificity and functions of the human V\u03b41+\u2003T cells remain obscure. We sought to explore the biological role for this T cell subset by investigating the reconstitution of T cell receptor (TCR) repertoires of V\u03b41+\u2003\u03b3\u03b4\u2003T lymphocytes after human allogeneic haematopoietic stem cell transplantation (HSCT). We observed skewed TCR repertoires of the V\u03b41+\u2003T cells in 27 of 44 post-transplant patients. Only one patient developed EBV-associated post-transplant lymphoproliferative disorder in the present patient cohort. The -WGI- amino acid motif was observed in CDR3 of clonally expanded V\u03b41+\u2003T cells in half the patients. A skew was also detected in certain healthy donors, and the V\u03b41+\u2003T cell clone derived from the donor mature T cell pool persisted in the recipient's blood even 10\u2003years after transplant. This T cell clone expanded in vitro against stimulation with autologous EBV\u2013lymphoblastoid cell lines (LCL), and the V\u03b41+\u2003T cell line expanded in vitro from the same patient showed cytotoxicity against autologous EBV\u2013LCL. EBV-infected cells could also induce in vitro oligoclonal expansions of autologous V\u03b41+\u2003T cells from healthy EBV-seropositive individuals. These results suggest that human V\u03b41+\u2003T cells have a TCR repertoire against EBV-infected B cells and may play a role in protecting recipients of allogeneic HSCT from EBV-associated disease.<\/jats:p>","DOI":"10.1111\/j.1365-2249.2007.03388.x","type":"journal-article","created":{"date-parts":[[2010,8,3]],"date-time":"2010-08-03T10:42:38Z","timestamp":1280832158000},"page":"70-79","source":"Crossref","is-referenced-by-count":80,"title":["Skewed T cell receptor repertoire of V\u03b41+\u2003\u03b3\u03b4\u2003T lymphocytes after human allogeneic haematopoietic stem cell transplantation and the potential role for Epstein\u2013Barr virus-infected B cells in clonal restriction"],"prefix":"10.1093","volume":"149","author":[{"given":"N","family":"Fujishima","sequence":"first","affiliation":[{"name":"Division of Hematology and Oncology, Department of Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Hirokawa","sequence":"additional","affiliation":[{"name":"Division of Hematology and Oncology, Department of Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Fujishima","sequence":"additional","affiliation":[{"name":"Division of Hematology and Oncology, Department of Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J","family":"Yamashita","sequence":"additional","affiliation":[{"name":"Radioisotope Division, Bioscience Center, Akita University School of Medicine, Akita, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H","family":"Saitoh","sequence":"additional","affiliation":[{"name":"Division of Hematology and Oncology, Department of Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y","family":"Ichikawa","sequence":"additional","affiliation":[{"name":"Division of Hematology and Oncology, Department of Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T","family":"Horiuchi","sequence":"additional","affiliation":[{"name":"Division of Hematology and Oncology, Department of Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y","family":"Kawabata","sequence":"additional","affiliation":[{"name":"Division of Hematology and Oncology, Department of Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K-I","family":"Sawada","sequence":"additional","affiliation":[{"name":"Division of Hematology and Oncology, Department of Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2007,4,11]]},"reference":[{"key":"2022010406293357900_b1","doi-asserted-by":"crossref","first-page":"S32","DOI":"10.1067\/mai.2000.106638","article-title":"Delayed recovery of CDR3 complexity of the T cell receptor \u03b2 chain in recipients of allogeneic bone marrow transplants who had virus-associated interstitial pneumonia: monitor of T-cell function by CDR3 spectratyping","volume":"106","author":"Hirokawa","year":"2000","journal-title":"J Allergy Clin Immunol"},{"key":"2022010406293357900_b2","doi-asserted-by":"crossref","first-page":"759","DOI":"10.1046\/j.1365-2141.2000.02080.x","article-title":"Restricted usage of T-cell receptor \u03b1-chain variable region (TCRAV) and T-cell receptor \u03b2-chain variable region (TCRBV) repertoires after human allogeneic haematopoietic transplantation","volume":"109","author":"Matsutani","year":"2000","journal-title":"Br J Haematol"},{"key":"2022010406293357900_b3","doi-asserted-by":"crossref","first-page":"731","DOI":"10.1038\/sj.bmt.1702859","article-title":"Identification of the T-cell clones expanding within both CD8+CD28+ and CD8+CD28- T-cell subsets in recipients of allogeneic hematopoietic cell grafts and its implication in post-transplant skewing of T-cell receptor repertoire","volume":"27","author":"Horiuchi","year":"2001","journal-title":"Bone Marrow Transplant"},{"key":"2022010406293357900_b4","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1038\/sj.bmt.1702858","article-title":"Extensive clonal expansion of T lymphocytes causes contracted diversity of the complementarity-determining region 3 and a skewing of T-cell receptor repertoires after allogeneic hematopoietic cell transplantation","volume":"27","author":"Hirokawa","year":"2001","journal-title":"Bone Marrow Transplant"},{"key":"2022010406293357900_b5","doi-asserted-by":"crossref","first-page":"1626","DOI":"10.1038\/sj.leu.2402994","article-title":"The presence and longevity of peripherally expanded donor-derived TCR\u03b1\u03b2+ mature T lymphocyte clones after allogeneic bone marrow transplantation for adult myeloid leukemias","volume":"17","author":"Saitoh","year":"2003","journal-title":"Leukemia"},{"key":"2022010406293357900_b6","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1016\/0167-5699(89)90321-6","article-title":"Subpopulations of human peripheral \u03b3\u03b4T lymphocytes","volume":"10","author":"Triebel","year":"1989","journal-title":"Immunol Today"},{"key":"2022010406293357900_b7","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1038\/sj.bmt.1702478","article-title":"Reconstitution of \u03b3\u03b4 T-cell repertoire diversity after human allogeneic hematopoietic cell transplantation and the role for peripheral expansion of mature T-cell population in the graft","volume":"26","author":"Hirokawa","year":"2000","journal-title":"Bone Marrow Transplant"},{"key":"2022010406293357900_b8","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1111\/j.1365-2249.1991.tb05612.x","article-title":"A subset of gamma delta lymphocytes is increased during HIV infection","volume":"83","author":"De Paoli","year":"1991","journal-title":"Clin Exp Immunol"},{"key":"2022010406293357900_b9","first-page":"206","article-title":"T cell receptor gamma delta lymphocyte subsets during HIV infection","volume":"75","author":"Autran","year":"1989","journal-title":"Clin Exp Immunol"},{"key":"2022010406293357900_b10","doi-asserted-by":"crossref","first-page":"917","DOI":"10.1093\/infdis\/165.5.917","article-title":"Selective increase of a subset of T cell receptor gamma delta T lymphocytes in the peripheral blood of patients with human immunodeficiency virus type 1 infection","volume":"165","author":"De Maria","year":"1992","journal-title":"J Infect Dis"},{"key":"2022010406293357900_b11","doi-asserted-by":"crossref","first-page":"1437","DOI":"10.1172\/JCI5409","article-title":"Implication of \u03b3\u03b4 T cells in the human immune response to cytomegalovirus","volume":"103","author":"Dechanet","year":"1999","journal-title":"J Clin Invest"},{"key":"2022010406293357900_b12","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1086\/314568","article-title":"Major expansion of \u03b3\u03b4 T lymphocytes following cytomegalovirus infection in kidney allograft recipients","volume":"179","author":"Dechanet","year":"1999","journal-title":"J Infect Dis"},{"key":"2022010406293357900_b13","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1086\/322843","article-title":"Cytomegalovirus infection in transplant recipients resolves when circulating \u03b3\u03b4 T lymphocytes expand, suggesting a protective antiviral role","volume":"184","author":"Lafarge","year":"2001","journal-title":"J Infect Dis"},{"key":"2022010406293357900_b14","doi-asserted-by":"crossref","first-page":"3190","DOI":"10.1128\/IAI.69.5.3190-3196.2001","article-title":"Perturbation and proinflammatory type activation of V delta 1(+) gamma delta T cells in African children with Plasmodium falciparum malaria","volume":"69","author":"Hviid","year":"2001","journal-title":"Infect Immun"},{"key":"2022010406293357900_b15","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1007\/s002770050483","article-title":"Clonal expansion of \u03b3\u03b4-T lymphocytes in an HTLV-I carrier, associated with chronic neutropenia and rheumatoid arthritis","volume":"78","author":"Horiuchi","year":"1999","journal-title":"Ann Hematol"},{"key":"2022010406293357900_b16","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/0022-1759(93)90193-B","article-title":"Detection of human T cell receptor cDNAs (\u03b1, \u03b2, \u03b3 and \u03b4) by ligation of a universal adaptor to variable region","volume":"161","author":"Tsuruta","year":"1993","journal-title":"J Immunol Meth"},{"key":"2022010406293357900_b17","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1093\/intimm\/2.8.775","article-title":"Induction of human TCR\u03b3\u03b4+ and TCR\u03b3\u03b4\u2013CD2+CD3\u2013 double negative lymphocytes by bacterial stimulation","volume":"2","author":"Abo","year":"1990","journal-title":"Int Immunol"},{"key":"2022010406293357900_b18","first-page":"607","volume-title":"Hematopoietic cell transplantation","author":"Ambinder","year":"1999"},{"key":"2022010406293357900_b19","doi-asserted-by":"crossref","first-page":"3984","DOI":"10.4049\/jimmunol.149.12.3984","article-title":"V\u03b41+ subset of human \u03b3\u03b4 T cells respond to ligands expressed by EBV-infected Burkitt lymphoma cells and transformed B lymphocytes","volume":"149","author":"Hacker","year":"1992","journal-title":"J Immunol"},{"key":"2022010406293357900_b20","doi-asserted-by":"crossref","first-page":"652","DOI":"10.1046\/j.1365-3083.1996.d01-271.x","article-title":"The effects of mitogens, IL-2 and anti-CD3 antibody on the T-cell receptor V beta repertoire","volume":"43","author":"Jason","year":"1996","journal-title":"Scand J Immunol"},{"key":"2022010406293357900_b21","doi-asserted-by":"crossref","first-page":"1418","DOI":"10.4049\/jimmunol.154.3.1418","article-title":"CDR3-independent gamma delta V delta 1+ T cell expansion in the peripheral blood of HIV-infected persons","volume":"154","author":"Boullier","year":"1995","journal-title":"J Immunol"},{"key":"2022010406293357900_b22","doi-asserted-by":"crossref","first-page":"464","DOI":"10.4049\/jimmunol.158.1.464","article-title":"Evidence for B cell-mediated activation of V\u03b41+ T lymphocytes during progression of HIV infection","volume":"158","author":"Hyjek","year":"1997","journal-title":"J Immunol"},{"key":"2022010406293357900_b23","doi-asserted-by":"crossref","first-page":"876","DOI":"10.1016\/0140-6736(91)90203-2","article-title":"Circulating Epstein\u2013Barr virus-carrying B cells in acute malaria","volume":"337","author":"Lam","year":"1991","journal-title":"Lancet"},{"key":"2022010406293357900_b24","doi-asserted-by":"crossref","first-page":"1233","DOI":"10.1086\/428910","article-title":"Exposure to holoendemic malaria results in elevated Epstein\u2013Barr virus loads in children","volume":"191","author":"Moormann","year":"2005","journal-title":"J Infect Dis"},{"key":"2022010406293357900_b25","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1111\/j.1365-3083.1993.tb01735.x","article-title":"A subset of V delta 1+ T cells proliferates in response to Epstein\u2013Barr virus-transformed B cell lines in vitro","volume":"38","author":"Orsini","year":"1993","journal-title":"Scand J Immunol"},{"key":"2022010406293357900_b26","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1084\/jem.178.1.121","article-title":"Peripheral selection of V delta 1+ cells with restricted T cell receptor delta gene junctional repertoire in the peripheral blood of healthy donors","volume":"178","author":"Beldjord","year":"1993","journal-title":"J Exp Med"},{"key":"2022010406293357900_b27","doi-asserted-by":"crossref","first-page":"1914","DOI":"10.1002\/eji.1830240830","article-title":"Clonal expansions of V delta 1+ and V delta 2+ cells increase with age and limit the repertoire of human gamma delta T cells","volume":"24","author":"Giachino","year":"1994","journal-title":"Eur J Immunol"},{"key":"2022010406293357900_b28","doi-asserted-by":"crossref","first-page":"797","DOI":"10.1093\/intimm\/12.6.797","article-title":"High frequency of circulating gamma delta T cells with dominance of the v(delta) 1 subset in a healthy population","volume":"12","author":"Hviid","year":"2000","journal-title":"Int Immunol"},{"key":"2022010406293357900_b29","doi-asserted-by":"crossref","first-page":"3538","DOI":"10.4049\/jimmunol.154.7.3538","article-title":"Analysis of clonal CD8+ T cell expansions in normal individuals and patients with rheumatoid arthritis","volume":"154","author":"Fitzgerald","year":"1995","journal-title":"J Immunol"},{"key":"2022010406293357900_b30","doi-asserted-by":"crossref","first-page":"1407","DOI":"10.1093\/intimm\/10.10.1407","article-title":"The distribution of human TCR junctional region lengths shifts with age in both CD4 and CD8 T cells","volume":"10","author":"Hall","year":"1998","journal-title":"Int Immunol"},{"key":"2022010406293357900_b31","doi-asserted-by":"crossref","first-page":"1984","DOI":"10.4049\/jimmunol.169.4.1984","article-title":"Cytomegalovirus seropositivity drives the CD8 T cell repertoire toward greater clonality in healthy elderly individuals","volume":"169","author":"Khan","year":"2002","journal-title":"J Immunol"},{"key":"2022010406293357900_b32","doi-asserted-by":"crossref","first-page":"937","DOI":"10.1084\/jem.191.6.937","article-title":"Self-recognition of CD1 by \u03b3\/\u03b4 T cells: implications for innate immunity","volume":"191","author":"Spada","year":"2000","journal-title":"J Exp Med"},{"key":"2022010406293357900_b33","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1182\/blood.V72.1.241.241","article-title":"CD1c but neither CD1a nor CD1b molecules are expressed on normal, activated, and malignant human B cells: identification of a new B-cell subset","volume":"72","author":"Delia","year":"1988","journal-title":"Blood"}],"container-title":["Clinical and Experimental Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1365-2249.2007.03388.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/academic.oup.com\/cei\/article-pdf\/149\/1\/70\/42037984\/j.1365-2249.2007.03388.x.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/cei\/article-pdf\/149\/1\/70\/42037984\/j.1365-2249.2007.03388.x.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,6,2]],"date-time":"2023-06-02T15:09:18Z","timestamp":1685718558000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/cei\/article\/149\/1\/70\/6457810"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,4,11]]},"references-count":33,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2007,4,11]]},"published-print":{"date-parts":[[2007,4,25]]}},"URL":"https:\/\/doi.org\/10.1111\/j.1365-2249.2007.03388.x","relation":{},"ISSN":["0009-9104","1365-2249"],"issn-type":[{"value":"0009-9104","type":"print"},{"value":"1365-2249","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2007,7]]},"published":{"date-parts":[[2007,4,11]]}}}