{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,9]],"date-time":"2023-10-09T21:41:55Z","timestamp":1696887715654},"reference-count":12,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2007,4,25]],"date-time":"2007-04-25T00:00:00Z","timestamp":1177459200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2007,4,25]]},"abstract":"<jats:title>Summary<\/jats:title>\n               <jats:p>Apoptosis of blood monocytes was studied in experimental sepsis by multi-drug-resistant Pseudomonas aeruginosa. Thirty-six rabbits were used, divided into the following groups: A (n\u2003=\u20036), sham; B (n\u2003=\u20036), administered anaesthetics; and C (n\u2003=\u200324), acute pyelonephritis induced after inoculation of the test isolate in the renal pelvis. Blood was sampled at standard time intervals for estimation of tumour necrosis factor (TNF)-\u03b1 and isolation of monocytes. Half the monocytes were incubated and the other half was lysed for estimation of the cytoplasmic activity of caspase-3 by a kinetic chromogenic assay. No animal in groups A and B died; those in group C were divided into two subgroups, CI (n\u2003=\u20038) with present activity of caspase-3 of blood monocytes at 3\u00b75\u2003h and CII (n\u2003=\u200316) with absent activity. Their median survival was 2\u00b70 and 3\u00b75\u2003days, respectively (P\u2003=\u20030\u00b70089). Ex vivo secretion of TNF-\u03b1 from monocytes was higher by monocytes of subgroup CII than subgroup CI at 3\u00b75\u2003h (P\u2003=\u20030\u00b7039) and of group A than CII at 48\u2003h (P\u2003=\u20030\u00b7010). Median change of caspase-3 activity between 3\u00b75 and 24\u2003h of sampling was 56\u00b71 and \u22125\u00b78\u2003pmol\/min per 104 cells for subgroups CI and CII (P\u2003=\u20030\u00b7040), respectively. Respective changes between 3\u00b75 and 48\u2003h were 28\u2003981\u00b70 and 0\u2003pmol\/min per 104 cells (P\u2003=\u20030\u00b7036). Early induction of apoptosis in blood monocytes is of prime importance for the survival of the septic host and might be connected to changes of monocyte potential for the secretion of TNF-\u03b1.<\/jats:p>","DOI":"10.1111\/j.1365-2249.2007.03392.x","type":"journal-article","created":{"date-parts":[[2010,8,12]],"date-time":"2010-08-12T13:58:21Z","timestamp":1281621501000},"page":"103-108","source":"Crossref","is-referenced-by-count":9,"title":["Early apoptosis of blood monocytes is a determinant of survival in experimental sepsis by multi-drug-resistant <i>Pseudomonas aeruginosa<\/i>"],"prefix":"10.1093","volume":"149","author":[{"given":"A","family":"Antonopoulou","sequence":"first","affiliation":[{"name":"4th Department of Internal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Raftogiannis","sequence":"additional","affiliation":[{"name":"4th Department of Internal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E 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Internal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H","family":"Giamarellou","sequence":"additional","affiliation":[{"name":"4th Department of Internal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E E","family":"Douzinas","sequence":"additional","affiliation":[{"name":"3rd Department of Critical Care, University of Athens, Medical School, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2007,5,4]]},"reference":[{"key":"2022010406292298200_b1","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1080\/00365540310015692","article-title":"Role of apoptotic cell death in sepsis","volume":"35","author":"Hotchkiss","year":"2003","journal-title":"Scand J Infect Dis"},{"key":"2022010406292298200_b2","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1080\/00365540310015683","article-title":"Toll-like receptors and their signaling 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