{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,5]],"date-time":"2026-03-05T13:36:50Z","timestamp":1772717810528,"version":"3.50.1"},"reference-count":54,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2007,5,22]],"date-time":"2007-05-22T00:00:00Z","timestamp":1179792000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2007,5,22]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>The guinea pig model of low-dose pulmonary tuberculosis has been used to study the pathogenesis of infection as well as the mechanisms of bacille Calmette\u2013Gu\u00e9rin (BCG) vaccine-induced resistance. We investigated the function of lung cells from naive and BCG-vaccinated guinea pigs after enzymatic digestion of lung tissue with collagenase and DNase I. The total lung digest cells proliferated poorly to purified protein derivative (PPD) but comparatively better to ConA as assessed by [3H]-thymidine uptake. However, the non-adherent population obtained after plastic adherence of lung digests showed an enhanced response to concanavalin A (ConA) and PPD. Therefore, proliferation to ConA and PPD of nylon wool-purified T cells co-cultured with peritoneal (PM\u00f8s), alveolar (AM\u00f8s) or lung macrophages (LM\u00f8s) was assessed. Co-cultures of lung T cells and PM\u00f8s showed maximum proliferation to PPD, whereas proliferation was suppressed significantly by the addition of AM\u00f8s or LM\u00f8s. The response of T cells to ConA was unaffected in co-cultures. Incubation of co-cultures with recombinant guinea pig interferon-\u03b3 (rgpIFN-\u03b3) did not reverse the suppression. In contrast, rgpIFN-\u03b3-treated plastic adherent LM\u00f8s that were non-specific esterase-positive were capable of reducing the intracellular growth of Mycobacterium tuberculosis. Similarly, total, non-adherent and adherent lung digest cells from BCG-vaccinated guinea pigs showed IFN-\u03b3 and tumour necrosis factor (TNF)-\u03b1 mRNA expression in response to ConA, lipopolysaccharide or PPD by reverse transcription\u2013polymerase chain reaction followed by release of TNF protein but not IFN. These studies indicate that rgp-IFN-\u03b3-treated lung tissue macrophages from BCG-vaccinated guinea pigs are defective for inducing antigen-specific proliferation in T cells, but control the intracellular accumulation of virulent M. tuberculosis.<\/jats:p>","DOI":"10.1111\/j.1365-2249.2007.03425.x","type":"journal-article","created":{"date-parts":[[2009,10,13]],"date-time":"2009-10-13T21:27:50Z","timestamp":1255469270000},"page":"387-398","source":"Crossref","is-referenced-by-count":17,"title":["Lung macrophages from bacille Calmette\u2013Gu\u00e9rin-vaccinated guinea pigs suppress T cell proliferation but restrict intracellular growth of<i>M. tuberculosis<\/i>after recombinant guinea pig interferon-\u03b3 activation"],"prefix":"10.1093","volume":"149","author":[{"given":"A","family":"Jeevan","sequence":"first","affiliation":[{"name":"Department of Microbial and Molecular Pathogenesis, Texas A&M University System Health Science Center, College Station, TX, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K","family":"Majorov","sequence":"additional","affiliation":[{"name":"Laboratory for Immunogenetics, Central Institute for Tuberculosis, Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K","family":"Sawant","sequence":"additional","affiliation":[{"name":"Department of Microbial and Molecular Pathogenesis, Texas A&M University System Health Science Center, College Station, TX, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H","family":"Cho","sequence":"additional","affiliation":[{"name":"Department of Microbial and Molecular Pathogenesis, Texas A&M University System Health Science Center, College Station, TX, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D N","family":"McMurray","sequence":"additional","affiliation":[{"name":"Department of Microbial and Molecular Pathogenesis, Texas A&M University System Health Science Center, College Station, TX, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2007,6,12]]},"reference":[{"key":"2022010406292221700_b1","first-page":"324","article-title":"Towards new leprosy and tuberculosis vaccines","volume":"4","author":"Kaufmann","year":"1987","journal-title":"Microbiol Sci"},{"key":"2022010406292221700_b2","first-page":"8","article-title":"Epidemiology of tuberculosis","volume":"125","author":"Comstock","year":"1982","journal-title":"Am Rev Respir Dis"},{"key":"2022010406292221700_b3","doi-asserted-by":"crossref","first-page":"1024","DOI":"10.1093\/clinids\/8.6.1024","article-title":"Mycobacterial infections in AIDS patients, with an emphasis on the Mycobacterium avium complex","volume":"8","author":"Young","year":"1986","journal-title":"Rev Infect Dis"},{"key":"2022010406292221700_b4","volume-title":"The tubercle bacillus in the pulmonary lesion in man","author":"Canetti","year":"1955"},{"key":"2022010406292221700_b5","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1038\/nrmicro749","article-title":"Tuberculosis: a problem with persistence","volume":"1","author":"Stewart","year":"2003","journal-title":"Nat Rev Microbiol"},{"key":"2022010406292221700_b6","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/S0092-1157(79)80021-9","article-title":"Potency of 10 BCG vaccines as evaluated by their influence on the bacillemic phase of experimental airborne tuberculosis in guinea-pigs","volume":"7","author":"Smith","year":"1979","journal-title":"J Biol Stand"},{"key":"2022010406292221700_b7","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1007\/BF02751703","article-title":"BCG vaccination in India and tuberculosis in children: newer facets","volume":"61","author":"Udani","year":"1994","journal-title":"Indian J Pediatr"},{"key":"2022010406292221700_b8","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1016\/S0966-842X(00)88986-6","article-title":"BCG vaccination against tuberculosis: past disappointments and future hopes","volume":"3","author":"Roche","year":"1995","journal-title":"Trends Microbiol"},{"key":"2022010406292221700_b9","doi-asserted-by":"crossref","first-page":"149","DOI":"10.4049\/jimmunol.145.1.149","article-title":"Local production of tumor necrosis factor and IFN-gamma in tuberculous pleuritis","volume":"145","author":"Barnes","year":"1990","journal-title":"J Immunol"},{"key":"2022010406292221700_b10","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/S0145-2126(98)00155-6","article-title":"New data on the cytolytic effects of natural killer cells (Kurloff cells) on a leukemic cell line (guinea pig L2C)","volume":"23","author":"Debout","year":"1999","journal-title":"Leuk Res"},{"key":"2022010406292221700_b11","first-page":"337","article-title":"The immunology of antituberculous immunity","volume":"97","author":"Mackaness","year":"1969","journal-title":"Am Rev Respir Dis"},{"key":"2022010406292221700_b12","doi-asserted-by":"crossref","first-page":"189","DOI":"10.4049\/jimmunol.148.1.189","article-title":"T lymphocytes mediating protection and cellular cytolysis during the course of Mycobacterium tuberculosis infection. Evidence for different kinetics and recognition of a wide spectrum of protein antigens","volume":"148","author":"Orme","year":"1992","journal-title":"J Immunol"},{"key":"2022010406292221700_b13","doi-asserted-by":"crossref","first-page":"2243","DOI":"10.1084\/jem.178.6.2243","article-title":"Disseminated tuberculosis in interferon gamma gene-disrupted mice","volume":"178","author":"Cooper","year":"1993","journal-title":"J Exp Med"},{"key":"2022010406292221700_b14","doi-asserted-by":"crossref","first-page":"731","DOI":"10.1016\/0092-8674(89)90676-4","article-title":"The inducing role of tumor necrosis factor in the development of bactericidal granulomas during BCG infection","volume":"56","author":"Kindler","year":"1989","journal-title":"Cell"},{"key":"2022010406292221700_b15","doi-asserted-by":"crossref","first-page":"2249","DOI":"10.1084\/jem.178.6.2249","article-title":"An essential role for interferon gamma in resistance to Mycobacterium tuberculosis infection","volume":"178","author":"Flynn","year":"1993","journal-title":"J Exp Med"},{"key":"2022010406292221700_b16","doi-asserted-by":"crossref","first-page":"6957","DOI":"10.4049\/jimmunol.167.12.6957","article-title":"A protective and agonistic function of IL-12p40 in mycobacterial infection","volume":"167","author":"Holscher","year":"2001","journal-title":"J Immunol"},{"key":"2022010406292221700_b17","doi-asserted-by":"crossref","first-page":"4560","DOI":"10.1073\/pnas.0400983101","article-title":"Human IL-23-producing type 1 macrophages promote but IL-10-producing type 2 macrophages subvert immunity to (myco)bacteria","volume":"101","author":"Verreck","year":"2004","journal-title":"Proc Natl Acad Sci USA"},{"key":"2022010406292221700_b18","doi-asserted-by":"crossref","first-page":"2675","DOI":"10.1128\/iai.58.8.2675-2677.1990","article-title":"Activation of tuberculostatic macrophage functions by gamma interferon, interleukin-4, and tumor necrosis factor","volume":"58","author":"Flesch","year":"1990","journal-title":"Infect Immun"},{"key":"2022010406292221700_b19","doi-asserted-by":"crossref","first-page":"1435","DOI":"10.1084\/jem.178.4.1435","article-title":"Mice that lack the interferon-gamma receptor have profoundly altered responses to infection with bacillus Calmette\u2013Guerin and subsequent challenge with lipopolysaccharide","volume":"178","author":"Kemijo","year":"1993","journal-title":"J Exp Med"},{"key":"2022010406292221700_b20","doi-asserted-by":"crossref","first-page":"2364","DOI":"10.1172\/JCI2901","article-title":"Mutation in the signal-transducing chain of the interferon-gamma receptor and susceptibility to mycobacterial infection","volume":"101","author":"Dorman","year":"1998","journal-title":"J Clin Invest"},{"key":"2022010406292221700_b21","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1183\/09031936.93.06010120","article-title":"Regulation of antigen presenting cell(s) in lung and airway tissues","volume":"6","author":"Holt","year":"1993","journal-title":"Eur Respir J"},{"key":"2022010406292221700_b22","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1378\/chest.82.3.266","article-title":"Human alveolar macrophages suppress the proliferative response of peripheral blood lymphocytes","volume":"82","author":"McCombs","year":"1982","journal-title":"Chest"},{"key":"2022010406292221700_b23","first-page":"142","article-title":"Alveolar macrophages from humans and rodents selectively inhibit T cell proliferation but permit T cell activation and cytokine secretion","volume":"84","author":"Upham","year":"1995","journal-title":"Immunology"},{"key":"2022010406292221700_b24","doi-asserted-by":"crossref","first-page":"1773","DOI":"10.1084\/jem.177.6.1773","article-title":"Inhibition of the immunosuppressive activity of resident pulmonary alveolar macrophages by granulocyte\/macrophage colony-stimulating factor","volume":"177","author":"Bilyk","year":"1993","journal-title":"J Exp Med"},{"key":"2022010406292221700_b25","first-page":"429","article-title":"Alveolar macrophages. I. Inhibition of lymphocyte proliferation by purified macrophages from rat lung","volume":"37","author":"Holt","year":"1979","journal-title":"Immunology"},{"key":"2022010406292221700_b26","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1016\/0008-8749(80)90020-9","article-title":"Alveolar macrophages. IV. Interspecies differences in activity in proliferating lymphocyte cultures","volume":"50","author":"Holt","year":"1980","journal-title":"Cell Immunol"},{"key":"2022010406292221700_b27","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1054\/tuld.1998.0014","article-title":"Suppression of lymphoproliferation by alveolar macrophages in the guinea pig","volume":"79","author":"Zhang","year":"1998","journal-title":"Tuber Lung Dis"},{"key":"2022010406292221700_b28","doi-asserted-by":"crossref","first-page":"4271","DOI":"10.1128\/IAI.71.8.4271-4277.2003","article-title":"Coordinate cytokine gene expression in vivo following induction of tuberculous pleurisy in guinea pigs","volume":"71","author":"Allen","year":"2003","journal-title":"Infect Immun"},{"key":"2022010406292221700_b29","doi-asserted-by":"crossref","first-page":"1367","DOI":"10.1128\/IAI.73.3.1367-1376.2005","article-title":"Recombinant guinea pig tumor necrosis factor alpha stimulates the expression of interleukin-12 and the inhibition of Mycobacterium tuberculosis growth in macrophages","volume":"73","author":"Cho","year":"2005","journal-title":"Infect Immun"},{"key":"2022010406292221700_b30","doi-asserted-by":"crossref","first-page":"1245","DOI":"10.1128\/IAI.70.3.1245-1253.2002","article-title":"Effect of BCG infection on interleukin-1\u03b2 and RANTES mRNA expression in guinea pig cells exposed to attenuated and virulent mycobacteria","volume":"70","author":"Jeevan","year":"2002","journal-title":"Infect Immun"},{"key":"2022010406292221700_b31","doi-asserted-by":"crossref","first-page":"354","DOI":"10.1128\/IAI.71.1.354-364.2003","article-title":"Differential expression of gamma interferon mRNA induced by attenuated and virulent Mycobacterium tuberculosis in guinea pig cells after Mycobacterium bovis BCG vaccination","volume":"71","author":"Jeevan","year":"2003","journal-title":"Infect Immun"},{"key":"2022010406292221700_b32","doi-asserted-by":"crossref","first-page":"7035","DOI":"10.1128\/IAI.71.12.7035-7042.2003","article-title":"Effect of Mycobacterium bovis BCG vaccination on Mycobacterium-specific cellular proliferation and tumor necrosis factor alpha production from distinct guinea pig leukocyte populations","volume":"71","author":"Lasco","year":"2003","journal-title":"Infect Immun"},{"key":"2022010406292221700_b33","doi-asserted-by":"crossref","first-page":"5471","DOI":"10.1128\/IAI.70.10.5471-5478.2002","article-title":"Mycobacterium bovis BCG vaccination augments interleukin-8 mRNA expression and protein production in guinea pig alveolar macrophages infected with Mycobacterium tuberculosis","volume":"70","author":"Lyons","year":"2002","journal-title":"Infect Immun"},{"key":"2022010406292221700_b34","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1128\/IAI.74.1.213-224.2006","article-title":"Production and characterization of guinea pig recombinant gamma interferon and its effect on macrophage activation","volume":"74","author":"Jeevan","year":"2006","journal-title":"Infect Immun"},{"key":"2022010406292221700_b35","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1016\/j.tube.2005.01.001","article-title":"Evaluating the role of tumor necrosis factor-alpha in experimental pulmonary tuberculosis in the guinea pig","volume":"85","author":"Lasco","year":"2005","journal-title":"Tuberculosis"},{"key":"2022010406292221700_b36","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1016\/j.tube.2003.09.003","article-title":"Interleukin (IL)-8 (CXCL8) induces cytokine expression and superoxide formation by guinea pig neutrophils infected with Mycobacterium tuberculosis","volume":"84","author":"Lyons","year":"2004","journal-title":"Tuberculosis"},{"key":"2022010406292221700_b37","doi-asserted-by":"crossref","first-page":"1229","DOI":"10.1189\/jlb.0704414","article-title":"Recombinant guinea pig CCL5 (RANTES) differentially modulates cytokine production in alveolar and peritoneal macrophages","volume":"7","author":"Skwor","year":"2004","journal-title":"J Leukoc Biol"},{"key":"2022010406292221700_b38","doi-asserted-by":"crossref","first-page":"2666","DOI":"10.1128\/IAI.69.4.2666-2674.2001","article-title":"Gamma interferon-producing CD4+ T lymphocytes in the lung correlate with resistance to infection with Mycobacterium tuberculosis","volume":"69","author":"Chackerian","year":"2001","journal-title":"Infect Immun"},{"issue":"10","key":"2022010406292221700_b39","doi-asserted-by":"crossref","first-page":"5921","DOI":"10.4049\/jimmunol.165.10.5921","article-title":"Comparative analysis of T lymphocytes recovered from the lungs of mice genetically susceptible, resistant, and hyperresistant to Mycobacterium tuberculosis-triggered disease","volume":"165","author":"Lyadova","year":"2000","journal-title":"J Immunol"},{"key":"2022010406292221700_b40","doi-asserted-by":"crossref","first-page":"8437","DOI":"10.1128\/IAI.73.12.8437-8441.2005","article-title":"Neutralization of tumor necrosis factor alpha suppresses antigen-specific type 1 cytokine responses and reverses the inhibition of mycobacterial survival in cocultures of immune guinea pig T lymphocytes and infected macrophages","volume":"73","author":"Cho","year":"2005","journal-title":"Infect Immun"},{"key":"2022010406292221700_b41","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1093\/ajcp\/55.3.283","article-title":"Cytochemical identification of monocytes and granulocytes","volume":"55","author":"Yam","year":"1971","journal-title":"Am J Clin Pathol"},{"key":"2022010406292221700_b42","doi-asserted-by":"crossref","first-page":"314","DOI":"10.1128\/iai.55.2.314-319.1987","article-title":"Effects of diet and genetics on Mycobacterium bovis BCG vaccine efficacy in inbred guinea pigs","volume":"55","author":"Cohen","year":"1987","journal-title":"Infect Immun"},{"key":"2022010406292221700_b43","doi-asserted-by":"crossref","first-page":"793","DOI":"10.1089\/107999002320271387","article-title":"A new assay system for guinea pig interferon biological activity","volume":"22","author":"Yamamoto","year":"2002","journal-title":"J Interferon Cytokine Res"},{"key":"2022010406292221700_b44","first-page":"S95","article-title":"Lung repair and granuloma formation. Tubercle bacilli stimulated neutrophils release chemotactic factors for monocytes","volume":"83","author":"Antony","year":"1983","journal-title":"Chest"},{"key":"2022010406292221700_b45","first-page":"478","article-title":"T cell dependent chronic neutrophilia during mycobacterial infections","volume":"78","author":"Appleberg","year":"1989","journal-title":"Clin Exp Immunol"},{"key":"2022010406292221700_b46","doi-asserted-by":"crossref","first-page":"1147","DOI":"10.1128\/IAI.72.2.1147-1149.2004","article-title":"Rapid accumulation of eosinophils in lung lesions in guinea pigs infected with Mycobacterium tuberculosis","volume":"72","author":"Lasco","year":"2004","journal-title":"Infect Immun"},{"key":"2022010406292221700_b47","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1007\/BF01488203","article-title":"Guinea pig Kurloff (NK-like) cells mediate TNF-dependent cytotoxic activity: analogy with NC effector cells","volume":"20","author":"Pouliot","year":"1996","journal-title":"Inflammation"},{"key":"2022010406292221700_b48","first-page":"261","article-title":"Down-regulation of immune responses in the lower respiratory tract: the role of alveolar macrophages","volume":"63","author":"Holt","year":"1986","journal-title":"Clin Exp Immunol"},{"key":"2022010406292221700_b49","doi-asserted-by":"crossref","first-page":"786","DOI":"10.1136\/thx.52.9.786","article-title":"Selective inhibition of T cell proliferation but not expression of effector function by human alveolar macrophages","volume":"52","author":"Upham","year":"1997","journal-title":"Thorax"},{"key":"2022010406292221700_b50","first-page":"31","article-title":"Cytokine modulation of the immunosuppressive phenotype of pulmonary alveolar macrophage populations","volume":"86","author":"Bilyk","year":"1995","journal-title":"Immunology"},{"key":"2022010406292221700_b51","doi-asserted-by":"crossref","first-page":"1130","DOI":"10.1002\/eji.1830260526","article-title":"Dissociation between cytokine mRNA expression and protein production in shigellosis","volume":"26","author":"Raqib","year":"1996","journal-title":"Eur J Immunol"},{"key":"2022010406292221700_b52","doi-asserted-by":"crossref","first-page":"1383","DOI":"10.1093\/intimm\/5.11.1383","article-title":"Induction of IFN-\u03b3 in macrophages by lipopolysaccharide","volume":"5","author":"Fultz","year":"1993","journal-title":"Int Immunol"},{"key":"2022010406292221700_b53","doi-asserted-by":"crossref","first-page":"1023","DOI":"10.1172\/JCI6224","article-title":"Macrophages are a significant source of type 1 cytokines during mycobacterial infection","volume":"103","author":"Wang","year":"1999","journal-title":"J Clin Invest"},{"key":"2022010406292221700_b54","doi-asserted-by":"crossref","first-page":"5149","DOI":"10.1128\/iai.65.12.5149-5156.1997","article-title":"Induction of gamma interferon production in human alveolar macrophages by Mycobacterium tuberculosis","volume":"65","author":"Fenton","year":"1997","journal-title":"Infect Immun"}],"container-title":["Clinical and Experimental Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1365-2249.2007.03425.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/academic.oup.com\/cei\/article-pdf\/149\/2\/387\/42037281\/j.1365-2249.2007.03425.x.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/cei\/article-pdf\/149\/2\/387\/42037281\/j.1365-2249.2007.03425.x.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,27]],"date-time":"2023-05-27T04:41:55Z","timestamp":1685162515000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/cei\/article\/149\/2\/387\/6457788"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,5,22]]},"references-count":54,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2007,6,12]]},"published-print":{"date-parts":[[2007,5,22]]}},"URL":"https:\/\/doi.org\/10.1111\/j.1365-2249.2007.03425.x","relation":{},"ISSN":["0009-9104","1365-2249"],"issn-type":[{"value":"0009-9104","type":"print"},{"value":"1365-2249","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2007,8]]},"published":{"date-parts":[[2007,5,22]]}}}