{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T20:33:58Z","timestamp":1791318838623,"version":"4.3.1"},"reference-count":28,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2007,7,5]],"date-time":"2007-07-05T00:00:00Z","timestamp":1183593600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2007,8,23]]},"abstract":"<jats:title>Summary<\/jats:title>\n               <jats:p>The role of T cells in the pathophysiology of chronic obstructive pulmonary disease (COPD) is not yet certain, although varying reports have shown increases in T helper 1 (Th1) and\/or Th2 cytokines in peripheral blood and bronchoalveolar lavage (BAL). No studies have examined cytokine production by intraepithelial T cells obtained by bronchial brushing (BB). Intracellular cytokine analysis of T cell subsets from peripheral blood, BAL and BB from smoker and ex-smoker COPD patients, COPD patients receiving inhaled corticosteroids and smoker and non-smoker control subjects was studied using multi-parameter flow cytometry. CD4\u2003:\u2003CD8 inversion was noted in the peripheral blood of smoker and ex-smoker COPD groups, in BAL and BB from smoker controls and BAL of COPD smokers. There was an increase in intracellular CD8+ T cell Th1 proinflammatory cytokines in some COPD groups in the peripheral blood and in CD8+ T cell tumour necrosis factor (TNF)-\u03b1 in some COPD groups and smoker controls in BAL and BB. There was an increase in proinflammatory cytokines in COPD smokers compared with ex-smokers and a decrease in COPD smokers receiving inhaled corticosteroids in the airways. There was a negative correlation between forced expiratory volume in 1\u2003s (FEV1) and the percentage of BAL and intraepithelial CD8+ T cells producing TNF-\u03b1. COPD patients exhibit systemic inflammation as evidenced by increased intracellular Th1 proinflammatory cytokines in blood, BAL and intraepithelial CD8+ T cells, whereas smoker controls showed localized Th1 response in the lung only. Systemic therapeutic targeting of TNF-\u03b1 production by CD8+ T cells may improve morbidity in COPD patients while targeting of TNF-\u03b1 in the lung may prevent smokers progressing to COPD.<\/jats:p>","DOI":"10.1111\/j.1365-2249.2007.03451.x","type":"journal-article","created":{"date-parts":[[2010,8,12]],"date-time":"2010-08-12T14:06:05Z","timestamp":1281621965000},"page":"22-29","source":"Crossref","is-referenced-by-count":130,"title":["Increased intracellular T helper 1 proinflammatory cytokine production in peripheral blood, bronchoalveolar lavage and intraepithelial T cells of COPD subjects"],"prefix":"10.1093","volume":"150","author":[{"given":"G","family":"Hodge","sequence":"first","affiliation":[{"name":"Department of Thoracic Medicine, Royal Adelaide Hospital, Adelaide"},{"name":"Haematology Department, Women's and Children's Hospital, North Adelaide, South Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J","family":"Nairn","sequence":"additional","affiliation":[{"name":"Department of Thoracic Medicine, Royal Adelaide Hospital, Adelaide"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Holmes","sequence":"additional","affiliation":[{"name":"Department of Thoracic Medicine, Royal Adelaide Hospital, Adelaide"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P N","family":"Reynolds","sequence":"additional","affiliation":[{"name":"Department of Thoracic Medicine, Royal Adelaide Hospital, Adelaide"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Hodge","sequence":"additional","affiliation":[{"name":"Department of Thoracic Medicine, Royal Adelaide Hospital, Adelaide"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2007,7,5]]},"reference":[{"key":"2022010406285604700_b1","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1371\/journal.pmed.0010020","article-title":"Characterisation of T lymphocytes in chronic obstructive pulmonary disease","volume":"1","author":"Barnes","year":"2004","journal-title":"Plos Med"},{"key":"2022010406285604700_b2","doi-asserted-by":"crossref","first-page":"672","DOI":"10.1183\/09031936.03.00040703","article-title":"Chronic obstructive pulmonary disease: molecular and cellular mechanisms","volume":"22","author":"Barnes","year":"2003","journal-title":"Eur Respir J"},{"key":"2022010406285604700_b3","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1002\/cyto.b.20020","article-title":"Flow-cytometric characterisation of cell populations in bronchoalveolar lavage (BAL) and bronchial brushings from patients with chronic obstructive pulmonary disease (COPD)","volume":"61","author":"Hodge","year":"2004","journal-title":"Clin Cytometry"},{"key":"2022010406285604700_b4","doi-asserted-by":"crossref","first-page":"458","DOI":"10.1016\/S0091-6749(99)70471-9","article-title":"Predominant Th1 cytokine pattern in peripheral blood from subjects with chronic obstructive pulmonary disease","volume":"103","author":"Majori","year":"1999","journal-title":"J Allergy Clin Immunol"},{"key":"2022010406285604700_b5","doi-asserted-by":"crossref","first-page":"602","DOI":"10.1183\/09031936.03.00046402","article-title":"Interleukin-13 and -4 expression in the central airways of smokers with chronic bronchitis","volume":"22","author":"Miotto","year":"2003","journal-title":"Eur Respir J"},{"key":"2022010406285604700_b6","doi-asserted-by":"crossref","first-page":"2220","DOI":"10.1164\/ajrccm.164.12.2009060","article-title":"Interleukin-4 and interleukin-5 gene expression and inflammation in the mucous-secreting glands and subepithelial of smokers with chronic bronchitis. 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