{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T03:06:51Z","timestamp":1793502411423,"version":"4.5.0"},"reference-count":19,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2007,7,30]],"date-time":"2007-07-30T00:00:00Z","timestamp":1185753600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2007,8,23]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>IgG subclasses of glutamic acid decarboxylase (GAD65) antibodies (GADA) may reflect the immunological state in the pancreas of GADA-positive patients with autoimmune diabetes. The use of biotin-conjugated antibodies and streptavidin Sepharose are used commonly in immunoprecipitation assays (IPA) based on 125I- or 35S-labelled antigens to capture IgG subclasses directed against IA-2 or GAD65. We have compared three different immunoprecipitation assays for the determination of GADA IgG subclasses. Two of the assays were based on the biotin and streptavidin systems provided in a solid (immobilized) or liquid (mobilized) phase binding environment. The third assay was based on N-hydroxysuccinimide (immobilized) interaction with primary amines (i.e. lysine residues) on the antibody. We found the liquid phase binding assay (LPBA) to be the most stable assay, with a comparatively low coefficient of variation and background.<\/jats:p>","DOI":"10.1111\/j.1365-2249.2007.03473.x","type":"journal-article","created":{"date-parts":[[2010,8,12]],"date-time":"2010-08-12T14:24:16Z","timestamp":1281623056000},"page":"68-74","source":"Crossref","is-referenced-by-count":3,"title":["Determination of glutamic acid decarboxylase antibodies (GADA) IgG subclasses\u2003\u2212\u2003comparison of three immunoprecipitation assays (IPAs)"],"prefix":"10.1093","volume":"150","author":[{"given":"M","family":"Hillman","sequence":"first","affiliation":[{"name":"Department of Clinical Sciences, Lund University"},{"name":"Diabetes Research Laboratory, Lund University Hospital, Lund, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C","family":"T\u00f6rn","sequence":"additional","affiliation":[{"name":"Unit for Diabetes and Coeliac Disease, CRC, Malm\u00f6 University Hospital, Malm\u00f6, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Landin-Olsson","sequence":"additional","affiliation":[{"name":"Diabetes Research Laboratory, Lund University Hospital, Lund, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2007,7,30]]},"reference":[{"key":"2022010406285840700_b1","doi-asserted-by":"crossref","first-page":"S52","DOI":"10.2337\/diabetes.54.suppl_2.S52","article-title":"Autoantibodies in diabetes","volume":"54","author":"Pihoker","year":"2005","journal-title":"Diabetes"},{"key":"2022010406285840700_b2","doi-asserted-by":"crossref","first-page":"442","DOI":"10.1002\/1520-7560(2000)9999:9999<::AID-DMRR152>3.0.CO;2-T","article-title":"Glutamic acid decarboxylase antibodies (GADA) is the most important factor for prediction of insulin therapy within 3 years in young adult diabetic patients not classified as Type 1 diabetes on clinical 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