{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T00:54:16Z","timestamp":1787014456465,"version":"build-2736575974"},"reference-count":40,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2011,7,4]],"date-time":"2011-07-04T00:00:00Z","timestamp":1309737600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2011,8,2]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>Leishmaniasis is caused by infection with the protozoan parasite, Leishmania, that parasitizes human cells, and the cellular immune response is essential for controlling infection. In order to measure the host T cell response to Leishmania infection, we have measured the expansion, activation state and functional potential of specific T cells as identified by their T cell receptor V\u03b2 region expression. In a group of cutaneous leishmaniasis (CL) patients, we evaluated these characteristics in nine different T cell subpopulations as identified by their V\u03b2 region expression, before and after specific Leishmania antigen stimulation. Our results show: (1) an increase in CD4+ T cells expressing V\u03b2 5\u00b72 and V\u03b2 24 in CL compared to controls; (2) a Leishmania antigen-induced increase in CD4+ T cells expressing V\u03b2 5\u00b72, 11, 12 and 17; (3) a profile of previous activation of CD4+ V\u03b2 5\u00b72-, 11- and 24-positive T cells, with higher expression of CD45RO, HLA-DR, interferon-\u03b3, tumour necrosis factor-\u03b1 and interleukin-10 compared to other V\u03b2-expressing subpopulations; (4) a positive correlation between higher frequencies of CD4+V\u03b25\u00b72+ T cells and larger lesions; and (5) biased homing of CD4+ T cells expressing V\u03b2 5\u00b72 to the lesion site. Given that CL disease involves a level of pathology (ulcerated lesions) and is often followed by long-lived protection and cure, the identification of specific subpopulations active in this form of disease could allow for the discovery of immunodominant Leishmania antigens important for triggering efficient host responses against the parasite, or identify cell populations most involved in pathology.<\/jats:p>","DOI":"10.1111\/j.1365-2249.2011.04430.x","type":"journal-article","created":{"date-parts":[[2011,7,4]],"date-time":"2011-07-04T22:37:32Z","timestamp":1309819052000},"page":"338-351","source":"Crossref","is-referenced-by-count":15,"title":["CD4+ T cells defined by their V\u03b2 T cell receptor expression are associated with immunoregulatory profiles and lesion size in human leishmaniasis"],"prefix":"10.1093","volume":"165","author":[{"given":"T S L","family":"Keesen","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Immunology, Federal University of Minas Gerais"},{"name":"Institute for Education and Research, Santa Casa Hospital"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L R V","family":"Antonelli","sequence":"additional","affiliation":[{"name":"Ren\u00e9 Rachou Institute, FIOCRUZ"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D R","family":"Faria","sequence":"additional","affiliation":[{"name":"Department of Morphology, Federal University of Minas Gerais, Belo Horizonte, MG"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L H","family":"Guimar\u00e3es","sequence":"additional","affiliation":[{"name":"Immunology Service, Hospital Edgard Santos, UFBA-Salvador, Brazil"},{"name":"INCT-DT"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"O","family":"Bacellar","sequence":"additional","affiliation":[{"name":"Immunology Service, Hospital Edgard Santos, UFBA-Salvador, Brazil"},{"name":"INCT-DT"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E M","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Immunology Service, Hospital Edgard Santos, UFBA-Salvador, Brazil"},{"name":"INCT-DT"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W O","family":"Dutra","sequence":"additional","affiliation":[{"name":"Department of Morphology, Federal University of Minas Gerais, Belo Horizonte, MG"},{"name":"INCT-DT"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K J","family":"Gollob","sequence":"additional","affiliation":[{"name":"Institute for Education and Research, Santa Casa Hospital"},{"name":"INCT-DT"},{"name":"Center for Infections Disease Research, Biosciences Division, SRI International, Menlo Park, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2011,7,4]]},"reference":[{"key":"2021122708474049500_b1","doi-asserted-by":"crossref","first-page":"1561","DOI":"10.1016\/S0140-6736(05)67629-5","article-title":"Advances in 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X. Production and standardization of an antigen-induced mitogenic activity by peripheral blood mononuclear cells from treated, but not active cases of schistosomiasis","volume":"130","author":"Gazzinelli","year":"1983","journal-title":"J Immunol"},{"key":"2021122708474049500_b34","doi-asserted-by":"crossref","first-page":"6734","DOI":"10.1128\/IAI.70.12.6734-6740.2002","article-title":"Up-regulation of Th1-type responses in mucosal leishmaniasis patients","volume":"70","author":"Bacellar","year":"2002","journal-title":"Infect Immun"},{"key":"2021122708474049500_b35","doi-asserted-by":"crossref","first-page":"1457","DOI":"10.1126\/science.8451641","article-title":"TH1 and TH2 cell antigen receptors in experimental leishmaniasis","volume":"259","author":"Reiner","year":"1993","journal-title":"Science"},{"key":"2021122708474049500_b36","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1574-6968.2002.tb11101.x","article-title":"Type 1 and type 2 responses to Leishmania major","volume":"209","author":"Rogers","year":"2002","journal-title":"FEMS Microbiol Lett"},{"key":"2021122708474049500_b37","doi-asserted-by":"crossref","first-page":"2614","DOI":"10.1128\/iai.62.6.2614-2618.1994","article-title":"Leishmania-reactive CD4+ and CD8+ T cells associated with cure of human cutaneous leishmaniasis","volume":"62","author":"Da-Cruz","year":"1994","journal-title":"Infect Immun"},{"key":"2021122708474049500_b38","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1006\/expr.1996.0100","article-title":"T-cell responsiveness of American cutaneous leishmaniasis patients to purified Leishmania pifanoi amastigote antigens and Leishmania braziliensis promastigote antigens: immunologic patterns associated with cure","volume":"84","author":"Coutinho","year":"1996","journal-title":"Exp Parasitol"},{"key":"2021122708474049500_b39","first-page":"233","article-title":"Serum levels of tumor necrosis factor in patients with American cutaneous leishmaniasis","volume":"26","author":"Castes","year":"1993","journal-title":"Biol Res"},{"key":"2021122708474049500_b40","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1111\/j.1365-2249.2004.02479.x","article-title":"Phenotypic and functional characteristics of CD28+ and CD28- cells from chagasic patients: distinct repertoire and cytokine expression","volume":"137","author":"Menezes","year":"2004","journal-title":"Clin Exp Immunol"}],"container-title":["Clinical and Experimental Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1365-2249.2011.04430.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/academic.oup.com\/cei\/article-pdf\/165\/3\/338\/41908768\/j.1365-2249.2011.04430.x.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/cei\/article-pdf\/165\/3\/338\/41908768\/j.1365-2249.2011.04430.x.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,6,7]],"date-time":"2023-06-07T20:03:30Z","timestamp":1686168210000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/cei\/article\/165\/3\/338\/6422900"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,7,4]]},"references-count":40,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2011,7,4]]},"published-print":{"date-parts":[[2011,8,2]]}},"URL":"https:\/\/doi.org\/10.1111\/j.1365-2249.2011.04430.x","relation":{},"ISSN":["0009-9104","1365-2249"],"issn-type":[{"value":"0009-9104","type":"print"},{"value":"1365-2249","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2011,9]]},"published":{"date-parts":[[2011,7,4]]}}}