{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T07:27:59Z","timestamp":1789889279610,"version":"4.0.1"},"reference-count":31,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2012,1,30]],"date-time":"2012-01-30T00:00:00Z","timestamp":1327881600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2012,1,30]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Despite recent tissue-engineering advances, there is no effective way of replacing all the functions of the larynx in those requiring laryngectomy. A recent clinical transplant was a success. Using quantitative immunofluorescence targeted at immunologically relevant molecules, we have studied the early (48\u2003h and 1 week) immunological responses within larynxes transplantated between seven pairs of National Institutes of Health (NIH) minipigs fully homozygous at the major histocompatibility complex (MHC) locus. There were only small changes in expression of some molecules (relative to interindividual variation) and these were clearest in samples from the subglottic region, where the areas of co-expression of CD25+CD45RC-CD8- and of CD163+CD172+MHC-II- increased at 1 week after transplant. In one case, infiltration by recipient T cells was analysed by T cell receptor (TCR) V\u03b2 spectratype analysis; this suggested that changes in the T cell repertoire occur in the donor subglottis mucosal tissues from day 0 to day 7, but that the donor and recipient mucosal V\u03b2 repertoires remain distinct. The observed lack of strong immunological responses to the trauma of surgery and ischaemia provides encouraging evidence to support clinical trials of laryngeal transplantation, and a basis on which to interpret future studies involving mismatches.<\/jats:p>","DOI":"10.1111\/j.1365-2249.2011.04531.x","type":"journal-article","created":{"date-parts":[[2011,11,23]],"date-time":"2011-11-23T12:35:17Z","timestamp":1322051717000},"page":"556-564","source":"Crossref","is-referenced-by-count":14,"title":["Laryngeal transplantation in minipigs: early immunological outcomes"],"prefix":"10.1093","volume":"167","author":[{"given":"M A","family":"Birchall","sequence":"first","affiliation":[{"name":"Department of Clinical Sciences at South Bristol"},{"name":"North Bristol NHS Trust, Bristol, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S M","family":"Ayling","sequence":"additional","affiliation":[{"name":"Divisions of Veterinary Pathology, Infection and Immunity"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R","family":"Harley","sequence":"additional","affiliation":[{"name":"Divisions of Veterinary Pathology, Infection and Immunity"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P J","family":"Murison","sequence":"additional","affiliation":[{"name":"Companion Animals Science, Department of Clinical Veterinary Sciences, University of Bristol"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R","family":"Burt","sequence":"additional","affiliation":[{"name":"Divisions of Veterinary Pathology, Infection and Immunity"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L","family":"Mitchard","sequence":"additional","affiliation":[{"name":"Divisions of Veterinary Pathology, Infection and Immunity"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A","family":"Jones","sequence":"additional","affiliation":[{"name":"Companion Animals Science, Department of Clinical Veterinary Sciences, University of Bristol"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P","family":"Macchiarini","sequence":"additional","affiliation":[{"name":"Department of Thoracic Surgery, Faculty of Medicine, University of Barcelona, Hospital Clinic, Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C R","family":"Stokes","sequence":"additional","affiliation":[{"name":"Divisions of Veterinary Pathology, Infection and Immunity"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Bailey","sequence":"additional","affiliation":[{"name":"Divisions of Veterinary Pathology, Infection and Immunity"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2012,1,30]]},"reference":[{"key":"2021122708460791900_b1","volume-title":"National Head and Neck Cancer Audit: key findings for England and Wales for the audit period October 2006\u2013November 2007","author":"Data 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