{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T12:46:45Z","timestamp":1789390005888,"version":"build-2803163510"},"reference-count":41,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2012,3,2]],"date-time":"2012-03-02T00:00:00Z","timestamp":1330646400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2012,3,2]]},"abstract":"<jats:title>Summary<\/jats:title>\n               <jats:p>Excessive T helper type 1 (Th1) cell activity has been reported in Beh\u00e7et's disease (BD). Recently, association of Th17 cells with certain autoimmune diseases was reported, and we thus investigated circulating Th17 cells in BD. CD4+CD45RO\u2013 (naive) T cells were cultured with Th0-, Th1-, Th2- and Th17-related cytokines and antibodies, and their mRNA was studied by real-time polymerase chain reaction (PCR). When naive CD4+ T cells were cultured with Th1- and Th17-related cytokines, interferon (IFN)-\u03b3 mRNA and interleukin (IL)-17 mRNA were up-regulated, respectively, in BD patients. Naive CD4+ T cells cultured in a Th17 cell-inducing condition expressed IL-23 receptor (IL-23R) mRNA excessively. IL-17 mRNA expression was induced only when naive CD4+T cells were cultured in the presence of IL-23. CD4+ T cells cultured with Th17 cytokines expressed excessive RAR-related orphan receptor C (RORC) mRNA. Using intracellular cytokine staining, we found that CD45RO+(memory) CD4+ T cells producing IL-17 and IFN-\u03b3 simultaneously were increased significantly. Memory CD4+ T cells producing IFN-\u03b3 but not IL-17 decreased profoundly in BD patients. CD4+ T cells producing IL-17 and IFN-\u03b3 simultaneously were found in BD skin lesions. Collectively, we found excessive CD4+ T cells producing IL-17 and IFN-\u03b3 (Th1\/Th17) cells in patients with BD, and possible involvement of IL-23\/IL-23R pathway for the appearance of excessive Th1\/Th17 cells.<\/jats:p>","DOI":"10.1111\/j.1365-2249.2011.04543.x","type":"journal-article","created":{"date-parts":[[2011,12,9]],"date-time":"2011-12-09T08:56:35Z","timestamp":1323420995000},"page":"68-74","source":"Crossref","is-referenced-by-count":98,"title":["Excessive CD4+ T cells co-expressing interleukin-17 and interferon-\u03b3 in patients with Beh\u00e7et's disease"],"prefix":"10.1093","volume":"168","author":[{"given":"J","family":"Shimizu","sequence":"first","affiliation":[{"name":"Department of Immunology and Medicine, St Marianna University School of Medicine, Kawasaki"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K","family":"Takai","sequence":"additional","affiliation":[{"name":"Department of Immunology and Medicine, St Marianna University School of Medicine, Kawasaki"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N","family":"Fujiwara","sequence":"additional","affiliation":[{"name":"Department of Immunology and Medicine, St Marianna University School of Medicine, Kawasaki"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N","family":"Arimitsu","sequence":"additional","affiliation":[{"name":"Department of Immunology and Medicine, St Marianna University School of Medicine, Kawasaki"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y","family":"Ueda","sequence":"additional","affiliation":[{"name":"Department of Immunology and Medicine, St Marianna University School of Medicine, Kawasaki"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Wakisaka","sequence":"additional","affiliation":[{"name":"Department of Immunology and Medicine, St Marianna University School of Medicine, Kawasaki"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H","family":"Yoshikawa","sequence":"additional","affiliation":[{"name":"Department of Immunology and Medicine, St Marianna University School of Medicine, Kawasaki"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F","family":"Kaneko","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Fukushima Medical University, Fukushima, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Department of Immunology and Medicine, St Marianna University School of Medicine, Kawasaki"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Department of Immunology and Medicine, St Marianna University School of Medicine, 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