{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,12]],"date-time":"2023-10-12T05:40:25Z","timestamp":1697089225298},"reference-count":30,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,11,28]],"date-time":"2006-11-28T00:00:00Z","timestamp":1164672000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2007,2]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>An improved method for the diagnostic approach of <jats:italic>\u03b1<\/jats:italic><jats:sup>+<\/jats:sup>\u2010thalassaemia is described. The method is based on five common parameters: absence of iron deficiency, mild morphological abnormalities of erythrocytes, normal or slightly reduced erythrocytic indices MCV and MCH, normal chromatographic findings, and presence of haemoglobin H inclusions in erythrocytes with methyl\u2010violet stain after, but not before, incubation with oxidant agent. We studied by DNA analysis, 58 subjects fulfilling the above mentioned diagnostic criteria and we found that 50 of them (86.2%) had a <jats:italic>\u03b1<\/jats:italic>\u2010globin gene defect. In the remaining eight subjects (13.8%) no <jats:italic>\u03b1<\/jats:italic>\u2010gene defect could be documented with the techniques used in the DNA analysis, which detect the six well\u2010known <jats:italic>\u03b1<\/jats:italic><jats:sup>+<\/jats:sup>\u2010thalassaemic defects in the Greek population. We conclude that the improved method, we described has a high sensitivity and accuracy in the screening of <jats:italic>\u03b1<\/jats:italic><jats:sup>+<\/jats:sup>\u2010thalassaemia.<\/jats:p>","DOI":"10.1111\/j.1365-2257.2006.00826.x","type":"journal-article","created":{"date-parts":[[2006,11,28]],"date-time":"2006-11-28T15:03:50Z","timestamp":1164726230000},"page":"45-51","source":"Crossref","is-referenced-by-count":0,"title":["An improved method for the diagnostic approach of <i>\u03b1<\/i><sup>+<\/sup>\u2010thalassaemia"],"prefix":"10.1111","volume":"29","author":[{"given":"Z.","family":"REPAPINOU","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"PH.","family":"KARABABA","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"BOUSSIOU","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V.","family":"KAFARAKIS","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. 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