{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T02:49:43Z","timestamp":1778640583022,"version":"3.51.4"},"reference-count":30,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2006,7,6]],"date-time":"2006-07-06T00:00:00Z","timestamp":1152144000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Endocrinology"],"published-print":{"date-parts":[[2006,8]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p><jats:bold>Background\u2002<\/jats:bold> \u2002Prolactin (PRL) plays a central role in mammary gland development and lactation. Due to its molecular heterogeneity, measurement of PRL immunoreactivity does not necessarily reflect its intrinsic bioactivity. For many years the Nb2 rat lymphoma cell bioassay has been the only reference bioassay for human lactogens. This bioassay, however, does not always correlate with the clinical features found in some patients exhibiting normal or elevated immunoreactive serum PRL concentrations.<\/jats:p><jats:p><jats:bold>Objectives\u2002<\/jats:bold> \u2002(1) To determine the concentrations of bioactive PRL in serum samples from individuals with normoprolactinaemia or with different forms of hyperprolactinaemia using two recently described homologous <jats:italic>in vitro<\/jats:italic> bioassays (i.e. a transcriptional bioassay in HEK\u2010293 cells and a proliferation assay in Ba\/F3 cells); and (2) to compare these results with those generated by the classical Nb2 cell bioassay.<\/jats:p><jats:p><jats:bold>Design\u2002<\/jats:bold> \u2002Cross\u2010sectional study.<\/jats:p><jats:p><jats:bold>Setting\u2002<\/jats:bold> \u2002An institutional biomedical research laboratory.<\/jats:p><jats:p><jats:bold>Participants\u2002<\/jats:bold> \u2002Ten patients with symptomatic hyperprolactinaemia due to prolactinoma, 11 patients with asymptomatic hyperprolactinaemia and macroprolactinaemia, and nine normal women.<\/jats:p><jats:p><jats:bold>Main outcome measures\u2002<\/jats:bold> \u2002Measurement of immunoreactive and bioactive concentrations of serum PRL.<\/jats:p><jats:p><jats:bold>Results\u2002<\/jats:bold> \u2002Samples from normal women and patients with tumoral hyperprolactinaemia due to prolactinoma exhibited similar within\u2010group concentration values of bioactive and immunoreactive serum PRL when tested by the three bioassays and the immunoradiometric assay employed. By contrast, measurement of bioactive PRL in samples from patients with macroprolactinaemia revealed that macroprolactin was poorly active in the homologous receptor bioassays, while it was more active in the Nb2 bioassay.<\/jats:p><jats:p><jats:bold>Conclusions\u2002<\/jats:bold> \u2002The reduced bioactivity of PRL in patients with macroprolactinaemia may further explain the absence of clinical features of hyperprolactinaemia in these individuals. In addition, our findings indicate that species\u2010specificity and sensitivity of the bioassays are determinant factors in the measurement of the intrinsic biological activity of circulating PRL.<\/jats:p>","DOI":"10.1111\/j.1365-2265.2006.02548.x","type":"journal-article","created":{"date-parts":[[2006,7,7]],"date-time":"2006-07-07T13:07:04Z","timestamp":1152277624000},"page":"146-153","source":"Crossref","is-referenced-by-count":26,"title":["Application of new homologous <i>in vitro<\/i> bioassays for human lactogens to assess the actual bioactivity of human prolactin isoforms in hyperprolactinaemic patients"],"prefix":"10.1111","volume":"65","author":[{"given":"Alfredo","family":"Lea\u00f1os\u2010Miranda","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guadalupe","family":"C\u00e1rdenas\u2010Mondrag\u00f3n","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roxana","family":"Rivera\u2010Lea\u00f1os","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alfredo","family":"Ulloa\u2010Aguirre","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vincent","family":"Goffin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,6]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1210\/edrv.19.3.0334"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1210\/edrv-12-3-235"},{"key":"e_1_2_6_4_2","first-page":"385","article-title":"Sequence\u2010function relationships within the expanding family of prolactin, growth hormone, placental lactogen, and related proteins in mammals","volume":"17","author":"Goffin V.","year":"1996","journal-title":"Endocrine Reviews"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1097\/00006254-199008000-00003"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1210\/edrv-16-3-354"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1210\/jcem-48-6-1026"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1210\/jcem-69-3-585"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/1529-0131(200104)44:4<866::AID-ANR143>3.0.CO;2-6"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1210\/jcem-51-5-1058"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1385\/ENDO:20:1-2:177"},{"key":"e_1_2_6_12_2","doi-asserted-by":"crossref","first-page":"20110","DOI":"10.1016\/S0021-9258(18)54897-3","article-title":"A prolactin\u2010dependent immune cell line (Nb2) expresses a mutant form of prolactin receptor","volume":"266","author":"Ali S.","year":"1991","journal-title":"Journal of Biological Chemistry"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1210\/me.6.8.1242"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1210\/mend-4-8-1136"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.28.16573"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1994.tb06495.x"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0303-7207(03)00264-8"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1210\/jc.86.2.924"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1210\/jc.86.6.2619"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1191\/096120301672772070"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/37.9.1029"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1191\/096120398678920280"},{"key":"e_1_2_6_23_2","first-page":"1546","article-title":"Antiprolactin autoantibodies in systemic lupus erythematosus: frequency and correlation with prolactinemia and disease activity","volume":"28","author":"Lea\u00f1os\u2010Miranda A.","year":"2001","journal-title":"Journal of Rheumatology"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1385\/ENDO:19:2:139"},{"key":"e_1_2_6_25_2","first-page":"327","article-title":"Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter","volume":"1","author":"Southern P.J.","year":"1982","journal-title":"Journal of Molecular and Applied Gentics"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1210\/jcem-53-4-863"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1210\/jcem-61-2-258"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1210\/jc.81.2.586"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1530\/eje.0.1300434"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1210\/jc.82.9.3107"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2005-1831"}],"container-title":["Clinical Endocrinology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1365-2265.2006.02548.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1365-2265.2006.02548.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,13]],"date-time":"2023-10-13T21:49:58Z","timestamp":1697233798000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1365-2265.2006.02548.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,7,6]]},"references-count":30,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2006,8]]}},"alternative-id":["10.1111\/j.1365-2265.2006.02548.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1365-2265.2006.02548.x","archive":["Portico"],"relation":{},"ISSN":["0300-0664","1365-2265"],"issn-type":[{"value":"0300-0664","type":"print"},{"value":"1365-2265","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,7,6]]}}}