{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T05:52:23Z","timestamp":1790833943599,"version":"4.1.0"},"reference-count":22,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2011,12,7]],"date-time":"2011-12-07T00:00:00Z","timestamp":1323216000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Endocrinology"],"published-print":{"date-parts":[[2012,1]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p><jats:bold>Objective\u2002<\/jats:bold> Intravenous GnRH stimulation test has often been used as gold standard test for the evaluation of hypothalamic\u2013pituitary\u2013gonadal axis in the diagnosis of central precocious puberty (CPP) and in the assessment of pubertal suppression. However, this test is time\u2010consuming, costly and uncomfortable for the patients. We aimed to analyse the validity of single LH sample 90\u2003min after GnRH analogue (GnRHa) administration in the evaluation of gonadotrophin suppression during CPP therapy and to determine a cut\u2010off level for LH indicating adequate suppression.<\/jats:p><jats:p><jats:bold>Design\u2002<\/jats:bold> Prospective study.<\/jats:p><jats:p><jats:bold>Patients and Methods\u2002<\/jats:bold> One hundred and forty\u2010two patients with CPP were included in this study. Peak LH level during iv GnRH stimulation test after the third dose of GnRHa was compared with LH level 90\u2003min after injection of the 3rd dose of GnRHa.<\/jats:p><jats:p><jats:bold>Results\u2002<\/jats:bold> There was a positive correlation between LH level following a GnRHa injection and peak LH during standard iv GnRH stimulation test (<jats:italic>r<\/jats:italic>\u2003=\u20030\u00b783; <jats:italic>P\u2003<\/jats:italic>&lt;\u20030\u00b70001). A LH value of 2\u00b75\u2003mIU\/ml or less 90\u2003min after GnRHa injection was considered to be the cut\u2010off for the determination of pubertal suppression (sensitivity and specificity was 100% and 88%, respectively). In 117 patients, gonadotrophin suppression was existed according to both GnRHa and iv GnRH tests. In 25 patients, gonadotrophin suppression was not found in the GnRHa test. However, 16 of them were suppressed according to the iv GnRH test.<\/jats:p><jats:p><jats:bold>Conclusion\u2002<\/jats:bold> Single LH determination 90\u2003min after GnRHa administration using a cut\u2010off level of 2\u00b75\u2003mIU\/ml reflects pubertal suppression with a high sensitivity and specificity. However, this test may fail to show pubertal suppression in some cases. Those patients who appear to be inadequately suppressed should be reassessed using standard iv GnRH stimulation test for optimal dose adjustment.<\/jats:p>","DOI":"10.1111\/j.1365-2265.2011.04185.x","type":"journal-article","created":{"date-parts":[[2011,7,26]],"date-time":"2011-07-26T13:40:21Z","timestamp":1311687621000},"page":"126-130","source":"Crossref","is-referenced-by-count":23,"title":["Assessment of gonadotrophin suppression in girls treated with GnRH analogue for central precocious puberty; validity of single luteinizing hormone measurement after leuprolide acetate injection"],"prefix":"10.1111","volume":"76","author":[{"given":"Huseyin","family":"Demirbilek","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayfer","family":"Alikasifoglu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nazli E.","family":"Gonc","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alev","family":"Ozon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nurgun","family":"Kandemir","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2011,12,7]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1001\/archpedi.1994.02170040035006"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-3476(95)70254-7"},{"key":"e_1_2_6_4_2","first-page":"653","article-title":"Long\u2010term effectiveness of depot gonadotropin\u2010releasing hormone analogue in the treatment of children with central precocious puberty","volume":"147","author":"Clemons R.D.","year":"1993","journal-title":"American Journal of Diseases of Children"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2003-031537"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/dmh012"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1542\/peds.109.2.e30"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1136\/adc.44.235.291"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1097\/00000441-195909000-00030"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-3476(95)70255-5"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1210\/jc.84.12.4536"},{"key":"e_1_2_6_12_2","first-page":"223","article-title":"Advances in the treatment of precocious puberty","volume":"41","author":"Kappy M.S.","year":"1994","journal-title":"Advances in Pediatrics"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1210\/jc.74.5.1206"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1515\/JPEM.2009.22.2.161"},{"key":"e_1_2_6_15_2","first-page":"1061","article-title":"Efficacy of leuprolide therapy in children with central precocious puberty","volume":"142","author":"Kappy M.S.","year":"1988","journal-title":"American Journal of Diseases of Children"},{"key":"e_1_2_6_16_2","first-page":"109","article-title":"GnRH stimulation test in precocious puberty; single sample is adequate for diagnosis and dose adjustment","volume":"24","author":"Kandemir N.","year":"2011","journal-title":"Journal of Clinical Research in Pediatric Endocrinology"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11102-009-0184-0"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1159\/000023275"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1542\/peds.110.6.1255"},{"key":"e_1_2_6_20_2","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1177\/03000605900180S107","article-title":"Pharmaceutical manipulation of leuprorelin acetate to improve clinical performance","volume":"18","author":"Toguchi H.","year":"1990","journal-title":"Journal of International Medical Research"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1210\/jc.78.1.30"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1542\/peds.2008-1783"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2431-8-27"}],"container-title":["Clinical Endocrinology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1365-2265.2011.04185.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1365-2265.2011.04185.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,31]],"date-time":"2023-10-31T18:32:26Z","timestamp":1698777146000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1365-2265.2011.04185.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,12,7]]},"references-count":22,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2012,1]]}},"alternative-id":["10.1111\/j.1365-2265.2011.04185.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1365-2265.2011.04185.x","archive":["Portico"],"relation":{},"ISSN":["0300-0664","1365-2265"],"issn-type":[{"value":"0300-0664","type":"print"},{"value":"1365-2265","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,12,7]]}}}