{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T02:07:33Z","timestamp":1790215653485,"version":"4.1.0"},"reference-count":28,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2007,7,27]],"date-time":"2007-07-27T00:00:00Z","timestamp":1185494400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytopathology"],"published-print":{"date-parts":[[2007,10]]},"abstract":"<jats:p><jats:bold>Objective:\u2002<\/jats:bold> As cervical dysplasia may regress to normal cytology or progress to cervical carcinoma, it would be valuable to have a diagnostic tool to help decide whether therapeutic conization should be performed.<\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold> Cervical samples of 301 HPV16 positive women were collected in RNA<jats:italic>later<\/jats:italic> reagent to prevent RNA degradation. Relative levels of HPV16 DNA and HPV16 E6*I mRNA in the samples were determined using real\u2010time polymerase chain reaction. Findings were correlated with histological diagnoses and cytological follow\u2010up.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> HPV16 E6*I mRNA levels were significantly higher in women with cytologically diagnosed severe cervical dysplasia (mean normalized ratio = 0.25) than in those with mild\u2010to\u2010moderate dysplasia (mean norm. ratio = 0.12), atypical squamous cells of uncertain origin (mean norm. ratio = 0.071) or normal cytology (mean norm. ratio = 0.034). Viral DNA levels were not significantly different between severe and mild\u2010to\u2010moderate dysplasia (mean norm. ratios, 55.8 and 63.5, respectively). The PPV for a histological diagnosis of severe cervical dysplasia [cervical intraepithelial neoplasia (CIN) II+] increased with the amounts of E6*I mRNA to more than 90% whereas the sensitivity decreased. The absence of HPV16 E6*I transcripts as well as HPV16 DNA considerably increased the negative predictive value and the specificity. However, low concentrations (or complete absence) of E6*I mRNA did not preclude a CIN II+ diagnosis.<\/jats:p><jats:p><jats:bold>Conclusions:\u2002<\/jats:bold> Although the sensitivity is low, high levels of HPV16 E6*I mRNA are indicative of CIN II+ in cytologically diagnosed cervical dysplasia of individual patients. Thus, quantification of HPV16 E6*I mRNA could be helpful in managing follow\u2010up and treatment in a subset of HPV16 positive women.<\/jats:p>","DOI":"10.1111\/j.1365-2303.2007.00481.x","type":"journal-article","created":{"date-parts":[[2007,7,27]],"date-time":"2007-07-27T13:11:28Z","timestamp":1185541888000},"page":"290-299","source":"Crossref","is-referenced-by-count":20,"title":["Increased levels of HPV16 E6*I transcripts in high\u2010grade cervical cytology and histology (CIN II+) detected by rapid real\u2010time RT\u2010PCR amplification"],"prefix":"10.1111","volume":"18","author":[{"given":"S.","family":"K\u00f6sel","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Burggraf","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W.","family":"Engelhardt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Olgem\u00f6ller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2007,7,27]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjc.6600688"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa021641"},{"key":"e_1_2_6_4_2","first-page":"945","article-title":"Human papillomavirus testing for primary screening of cervical cancer precursors","volume":"9","author":"Ratnam S","year":"2000","journal-title":"Cancer Epidemiol Biomarkers Prev"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(04)17354-6"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199802123380703"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.1993.110"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(00)02401-6"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.10232"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.4.1030-1034.1999"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/path.1490"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1097\/00019606-200103000-00008"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.65.2.606-612.1991"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-1702(02)00190-9"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.20153"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1525-1578(10)60687-3"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.10439"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF03260048"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/1096-9071(200010)62:2<251::AID-JMV18>3.0.CO;2-V"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.281.17.1605"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.325.7364.572"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1158\/1055-9965.EPI-04-0410"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2003.11.035"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.50.7.600"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.2004.024570"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(00)02402-8"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-0934(97)00161-4"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910550311"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2006.07.032"}],"container-title":["Cytopathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1365-2303.2007.00481.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1365-2303.2007.00481.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,13]],"date-time":"2023-10-13T13:16:43Z","timestamp":1697203003000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1365-2303.2007.00481.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,7,27]]},"references-count":28,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2007,10]]}},"alternative-id":["10.1111\/j.1365-2303.2007.00481.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1365-2303.2007.00481.x","archive":["Portico"],"relation":{},"ISSN":["0956-5507","1365-2303"],"issn-type":[{"value":"0956-5507","type":"print"},{"value":"1365-2303","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,7,27]]}}}