{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T15:43:34Z","timestamp":1762098214845},"reference-count":27,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,2,25]],"date-time":"2005-02-25T00:00:00Z","timestamp":1109289600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Clin Investigation"],"published-print":{"date-parts":[[2005,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>Background\u2002<\/jats:bold> Several clinical trials have suggested that a metabolic cocktail of glucose\u2010insulin\u2010potassium (GIK) decreases mortality rates in patients with acute myocardial infarction (AMI). It has also been reported that Fas\u2010mediated apoptosis plays an important role in ischaemic\/reperfusion injury in the rat model. This study was designed to evaluate the interaction of ischaemic\/reperfusion and reperfusion therapy coadministered with high\u2010dose GIK treatment on soluble Fas\/APO\u20101 (sFas) and Fas ligand (sFasL) plasma concentration in patients with AMI.<\/jats:p><jats:p><jats:bold>Materials and methods\u2002<\/jats:bold> Seventy\u2010four patients presenting with AMI who underwent reperfusion therapy were randomized into a GIK group (<jats:italic>n<\/jats:italic>\u00a0=\u00a035) receiving high\u2010dose GIK for 24\u00a0h or a vehicle group (<jats:italic>n<\/jats:italic>\u00a0=\u00a039). Thirty\u2010four control subjects were also enrolled in the present study. Strepavidin\u2010biotin ELISA was used to determine the soluble sFas and sFasL plasma concentration at baseline, 24\u00a0h (h), 3\u00a0day (d), 7\u00a0d and 14\u00a0d.<\/jats:p><jats:p><jats:bold>Results\u2002<\/jats:bold> Soluble Fas and sFas\u2010L serum concentrations ([sFas] and [sFas\u2010L]) of patients with AMI were significantly elevated at baseline as compared with normal controls (NCs; <jats:italic>P\u00a0&lt;<\/jats:italic>\u00a00\u00b701 vs. NC). The sFas in the GIK and vehicle groups markedly decreased 24\u00a0h after the GIK infusion (10\u00b77\u21925\u00b79\u00a0ng\u00a0mL<jats:sup>\u22121<\/jats:sup> and 9\u00b77\u21926\u00b75\u00a0ng\u00a0mL<jats:sup>\u22121<\/jats:sup>; <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00\u00b701 vs. baseline) and then increased during the 3\u20137\u2010d period (5\u00b79\u219212\u00b71\u00a0ng\u00a0mL<jats:sup>\u22121<\/jats:sup> and 6\u00b75\u219211\u00b71\u00a0ng\u00a0mL<jats:sup>\u22121<\/jats:sup>; <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00\u00b701 vs. 24\u00a0h). The GIK group demonstrated reduced sFas (12\u00b71\u21925\u00b79\u00a0ng\u00a0mL<jats:sup>\u22121<\/jats:sup>) at 14\u00a0d (<jats:italic>P\u00a0&lt;<\/jats:italic>\u00a00\u00b701 vs. 7\u00a0d), with no concomitant changes in the vehicle group. The sFas\u2010L in the GIK and vehicle groups was not significant different during the 14\u2010d period.<\/jats:p><jats:p><jats:bold>Conclusions\u2002<\/jats:bold> These results indicate that the sFas and sFasL in patients with AMI increased significantly compared with NC. Owing to the cardioprotective effects reported here and by others, a high\u2010dose GIK infusion co\u2010administered with the timely re\u2010establishment of nutritive perfusion should be strongly considered as a treatment of choice for AMI. Additionally, sFas may be a valuable marker of the physiological response to ischaemic\/reperfusion injury and reperfusion associated with high\u2010dose GIK treatment.<\/jats:p>","DOI":"10.1111\/j.1365-2362.2005.01468.x","type":"journal-article","created":{"date-parts":[[2005,2,25]],"date-time":"2005-02-25T11:24:20Z","timestamp":1109330660000},"page":"164-170","source":"Crossref","is-referenced-by-count":26,"title":["High\u2010dose glucose\u2010insulin\u2010potassium treatment reduces myocardial apoptosis in patients with acute myocardial infarction"],"prefix":"10.1111","volume":"35","author":[{"given":"L.","family":"Zhang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y. H.","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. 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