{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T14:13:47Z","timestamp":1790518427868,"version":"4.1.0"},"reference-count":39,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2006,6,6]],"date-time":"2006-06-06T00:00:00Z","timestamp":1149552000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Clin Investigation"],"published-print":{"date-parts":[[2006,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>Background\u2002<\/jats:bold> Obese, insulin\u2010resistant individuals have raised levels of intestinal and hepatic lipoproteins. Insulin decreases the production of hepatic lipoproteins <jats:italic>in\u00a0vivo and<\/jats:italic> so this study aimed to investigate whether an acute hyperinsulinaemic, euglycaemic clamp could correct fasting and post\u2010prandial dyslipidaemia.<\/jats:p><jats:p><jats:bold>Subjects and methods\u2002<\/jats:bold> In a randomized, cross\u2010over design, post\u2010prandial lipaemia was compared in subjects infused either with insulin to achieve a steady\u2010state concentration of 100\u00a0mU\u00a0L<jats:sup>\u22121<\/jats:sup> or with saline. Nine obese (Body Mass Index\u00a0&gt;\u00a026\u00a0kg\u00a0m<jats:sup>\u22122<\/jats:sup>; waist\u00a0:\u00a0hip\u00a0&gt;\u00a01\u00b70) insulin\u2010resistant (Homeostatic Model Assessment score\u00a0&gt;\u00a02\u00b70) male subjects were given an oral fat load 3\u00a0h after the infusions began, and sampling continued for 6\u00a0h. Plasma apoB\u201048, triglyceride and nonesterified fatty acid (NEFA) were measured hourly.<\/jats:p><jats:p><jats:bold>Results\u2002<\/jats:bold> Average steady\u2010state serum insulin levels during the hyperinsulinaemic clamp were 123\u00a0\u00b1\u00a04\u00b74\u00a0mU\u00a0L<jats:sup>\u22121<\/jats:sup>. A paired analysis showed no net effect of insulin on post\u2010prandial chylomicron metabolism when calculated as the (apoB\u201048) incremental area under the curve (IAUC). However, there was a trend towards a delay in the apoB\u201048 peak, consistent with possible changes in the rates of chylomicron biogenesis, lipolysis and\/or clearance. Similarly, post\u2010prandial lipaemia (depicted as triglyceride IAUC) was similar for subjects infused with insulin or saline, but the peak post\u2010prandial response was delayed during insulin infusion. The NEFA were rapidly decreased by 83% after 3\u00a0h of insulin infusion.<\/jats:p><jats:p><jats:bold>Conclusions\u2002<\/jats:bold> In obesity and insulin resistance, short\u2010term changes in plasma insulin do not appreciably exert a regulatory effect on exogenously\u2010derived post\u2010prandial lipoproteins. The data suggest that hyperchylomicronaemia in insulin\u2010resistant subjects is a result of chronic aberrations in insulin\u2010mediated regulation of post\u2010prandial lipid metabolism.<\/jats:p>","DOI":"10.1111\/j.1365-2362.2006.01651.x","type":"journal-article","created":{"date-parts":[[2006,6,7]],"date-time":"2006-06-07T09:44:01Z","timestamp":1149673441000},"page":"489-496","source":"Crossref","is-referenced-by-count":9,"title":["Effect of an acute hyperinsulinaemic clamp on post\u2010prandial lipaemia in subjects with insulin resistance"],"prefix":"10.1111","volume":"36","author":[{"given":"E. M.","family":"Allister","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. P.","family":"James","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G. F.","family":"Watts","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. H. R.","family":"Barrett","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. C. L.","family":"Mamo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,6,6]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.312.7036.935"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9150(87)90020-7"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9150(96)05832-7"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9150(94)90085-X"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1097\/00041433-200210000-00001"},{"issue":"2","key":"e_1_2_7_7_2","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1093\/ajcn\/76.2.311","article-title":"Evidence for impaired lipolysis in abdominally obese men: postprandial study of apolipoprotein B\u201048\u2010 and B\u2010100\u2010containing lipoproteins","volume":"76","author":"Couillard C","year":"2002","journal-title":"Am J Clin Nutr"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.2001.281.3.E626"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.85.1.37"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2362.1998.00284.x"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M200544200"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.47.5.779"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.17.7.1454"},{"issue":"6","key":"e_1_2_7_14_2","doi-asserted-by":"crossref","first-page":"833","DOI":"10.2337\/diab.42.6.833","article-title":"Effects of acute hyperinsulinemia on VLDL triglyceride and VLDL apoB production in normal weight and obese individuals","volume":"42","author":"Lewis GF","year":"1993","journal-title":"Diabetes"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI117633"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.44.9.1059"},{"key":"e_1_2_7_17_2","first-page":"253","article-title":"Acute hyperinsulinemia decreases hepatic secretion of very low\u2010density lipoprotein apolipoprotein B\u2010100 in normolipidemic, non\u2010diabetic subjects","volume":"3","author":"Watts GF","year":"1996","journal-title":"J Clin Endocrinol Metab"},{"issue":"2","key":"e_1_2_7_18_2","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1042\/bst0210509","article-title":"Regulation of lipoprotein lipase","volume":"21","author":"Olivecrona T","year":"1993","journal-title":"Biochem Soc Trans"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9150(78)90155-7"},{"key":"e_1_2_7_20_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1988.tb14084.x"},{"issue":"5","key":"e_1_2_7_21_2","doi-asserted-by":"crossref","first-page":"825","DOI":"10.1093\/ajcn\/47.5.825","article-title":"Serum triglyceride responses to fatty meals: effects of meal fat content","volume":"47","author":"Cohen JC","year":"1988","journal-title":"Am J Clin Nutr"},{"issue":"12","key":"e_1_2_7_22_2","doi-asserted-by":"crossref","first-page":"2387","DOI":"10.1016\/S0022-2275(20)33317-4","article-title":"Lipoprotein lipase transport in plasma: role of muscle and adipose tissues in regulation of plasma lipoprotein lipase concentrations","volume":"39","author":"Karpe F","year":"1998","journal-title":"J Lipid Res"},{"key":"e_1_2_7_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9150(99)00397-4"},{"key":"e_1_2_7_24_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.1979.237.3.E214"},{"key":"e_1_2_7_25_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9136(199807)15:7<539::AID-DIA668>3.0.CO;2-S"},{"key":"e_1_2_7_26_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2362.1999.00431.x"},{"key":"e_1_2_7_27_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1463-1326.2004.00407.x"},{"key":"e_1_2_7_28_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00280883"},{"key":"e_1_2_7_29_2","doi-asserted-by":"publisher","DOI":"10.2337\/diacare.23.1.57"},{"key":"e_1_2_7_30_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11745-001-0696-6"},{"issue":"1","key":"e_1_2_7_31_2","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/S0022-2275(20)34213-9","article-title":"Participation of the microsomal triglyceride transfer protein in lipoprotein assembly in Caco\u22122 cells: interaction with saturated and unsaturated dietary fatty acids","volume":"39","author":"Van Greevenbroek MM","year":"1998","journal-title":"J Lipid Res"},{"key":"e_1_2_7_32_2","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.52.5.1073"},{"key":"e_1_2_7_33_2","doi-asserted-by":"publisher","DOI":"10.1053\/meta.2002.33350"},{"key":"e_1_2_7_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9150(01)00721-3"},{"key":"e_1_2_7_35_2","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.50.2.462"},{"issue":"5","key":"e_1_2_7_36_2","doi-asserted-by":"crossref","first-page":"1073","DOI":"10.1016\/S0022-2275(20)39865-5","article-title":"Microsomal triglyceride transfer protein (MTP) regulation in HepG2 cells: insulin negatively regulates MTP gene expression","volume":"36","author":"Lin MC","year":"1995","journal-title":"J Lipid Res"},{"key":"e_1_2_7_37_2","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.54.6.1676"},{"key":"e_1_2_7_38_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02254364"},{"key":"e_1_2_7_39_2","doi-asserted-by":"publisher","DOI":"10.1053\/meta.2003.50032"},{"key":"e_1_2_7_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/0026-0495(92)90269-G"}],"container-title":["European Journal of Clinical Investigation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1365-2362.2006.01651.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1365-2362.2006.01651.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,13]],"date-time":"2023-10-13T06:50:53Z","timestamp":1697179853000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1365-2362.2006.01651.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,6,6]]},"references-count":39,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2006,7]]}},"alternative-id":["10.1111\/j.1365-2362.2006.01651.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1365-2362.2006.01651.x","archive":["Portico"],"relation":{},"ISSN":["0014-2972","1365-2362"],"issn-type":[{"value":"0014-2972","type":"print"},{"value":"1365-2362","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,6,6]]}}}