{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T01:03:39Z","timestamp":1777424619712,"version":"3.51.4"},"reference-count":47,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2011,7,12]],"date-time":"2011-07-12T00:00:00Z","timestamp":1310428800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Clin Investigation"],"published-print":{"date-parts":[[2012,1]]},"abstract":"<jats:sec>\n                    <jats:label\/>\n                    <jats:p>Eur J Clin Invest 2012; 42 (1): 49\u201360<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Abstract<\/jats:title>\n                    <jats:p>\n                      <jats:bold>Background\u2002<\/jats:bold>\n                      Osteoclasts play a critical role in bone resorption under basal conditions, but they also contribute to pathological bone loss during diseases including postmenopausal osteoporosis. Phospholipase C\u03b32 (PLC\u03b32) is an important signalling molecule in diverse haematopoietic lineages. Here, we tested the role of PLC\u03b32 in basal and ovariectomy\u2010induced bone resorption, as well as in\n                      <jats:italic>in vitro<\/jats:italic>\n                      osteoclast cultures using PLC\u03b32\u2010deficient (PLC\u03b32\n                      <jats:sup>\u2212\/\u2212<\/jats:sup>\n                      ) mice.\n                    <\/jats:p>\n                    <jats:p>\n                      <jats:bold>Materials and methods\u2002<\/jats:bold>\n                      The trabecular architecture of long bone metaphyses was tested by micro\u2010CT and histomorphometric analyses. Postmenopausal osteoporosis was modelled by surgical ovariectomy. Osteoclast development and function, gene expression and PLC\u03b32 phosphorylation were tested on\n                      <jats:italic>in vitro<\/jats:italic>\n                      osteoclast and macrophage cultures.\n                    <\/jats:p>\n                    <jats:p>\n                      <jats:bold>Results\u2002<\/jats:bold>\n                      PLC\u03b32\n                      <jats:sup>\u2212\/\u2212<\/jats:sup>\n                      mice had significantly higher trabecular bone mass under basal conditions than wild\u2010type mice. PLC\u03b32 was required for\n                      <jats:italic>in vitro<\/jats:italic>\n                      development and resorptive function of osteoclasts, but not for upregulation of osteoclast\u2010specific gene expression. PLC\u03b32 was phosphorylated in a Src\u2010family\u2010dependent manner upon macrophage adhesion but not upon stimulation by M\u2010CSF or RANKL. Surprisingly, ovariectomy\u2010induced bone resorption in PLC\u03b32\n                      <jats:sup>\u2212\/\u2212<\/jats:sup>\n                      mice was similar to, or even more robust than, that in wild\u2010type animals.\n                    <\/jats:p>\n                    <jats:p>\n                      <jats:bold>Conclusions\u2002<\/jats:bold>\n                      Our results indicate that PLC\u03b32 participates in bone resorption under basal conditions, likely because of its role in adhesion receptor signalling during osteoclast development. In contrast, PLC\u03b32 does not appear to play a major role in ovariectomy\u2010induced bone loss. These results suggest that basal and oestrogen deficiency\u2013induced bone resorption utilizes different signalling pathways and that PLC\u03b32 may not be a suitable therapeutic target in postmenopausal osteoporosis.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/j.1365-2362.2011.02556.x","type":"journal-article","created":{"date-parts":[[2011,5,19]],"date-time":"2011-05-19T01:41:22Z","timestamp":1305769282000},"page":"49-60","source":"Crossref","is-referenced-by-count":31,"title":["Phospholipase C\u03b32 is required for basal but not oestrogen deficiency\u2013induced bone 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