{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T15:52:13Z","timestamp":1784908333500,"version":"3.55.0"},"reference-count":26,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2011,9,13]],"date-time":"2011-09-13T00:00:00Z","timestamp":1315872000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Clin Investigation"],"published-print":{"date-parts":[[2012,4]]},"abstract":"<jats:sec><jats:label\/><jats:p>Eur J Clin Invest 2012; 42 (4): 365\u2013370<\/jats:p><\/jats:sec><jats:sec><jats:title>Abstract<\/jats:title><jats:p><jats:bold>Background\u2002<\/jats:bold> Apolipoprotein (apo) B\u2010containing lipoproteins are closely linked to atherogenesis. These lipoproteins are transported in plasma and are also associated with blood leucocytes. Our aim was to investigate whether apoB\u2010containing lipoproteins are also present on the surface of erythrocytes and investigate the relationship with the presence of atherosclerosis in a cross\u2010sectional study.<\/jats:p><jats:p><jats:bold>Materials and methods\u2002<\/jats:bold> Erythrocyte\u2010bound apoB (ery\u2010apoB) was measured by flowcytometry in subjects with (CAD+) and without coronary artery disease (CAD\u2212), based on coronary angiography or on a history of cardiovascular disease. Intima media thickness (IMT) measurements were carried out using B\u2010mode ultrasound. The relationship between ery\u2010apoB and clinical and subclinical atherosclerosis was evaluated with binary logistic regression.<\/jats:p><jats:p><jats:bold>Results\u2002<\/jats:bold> A total of 166 subjects were included (40 CAD+ and 126 CAD\u2212). ApoB was detected on freshly isolated erythrocytes (range: 0\u00b71\u20135\u00b75 au; mean\u2003\u00b1\u2003SEM 0\u00b786\u2003\u00b1\u20030\u00b709 au) in all but nine subjects (four CAD+ and five CAD\u2212). Ery\u2010apoB was lower in CAD+ (0\u00b762\u2003\u00b1\u20030\u00b709 au) compared to CAD\u2212 (1\u00b718\u2003\u00b1\u20030\u00b710 au; <jats:italic>P<\/jats:italic>\u2003&lt;\u20030\u00b7001). Higher ery\u2010apoB was associated with a lower risk of CAD (adjusted OR: 0\u00b7003 (95% CI: 0\u00b7001\u20130\u00b708; <jats:italic>P<\/jats:italic>\u2003&lt;\u20030\u00b7001), but the protective effect was diminished with increasing age (adjusted OR: 1\u00b710 (95% CI: 1\u00b704\u20131\u00b716; <jats:italic>P<\/jats:italic>\u2003&lt;\u20030\u00b7001). IMT was increased in CAD+ subjects (0\u00b777\u2003\u00b1\u20030\u00b713\u2003mm) compared to CAD\u2212 (0\u00b757\u2003\u00b1\u20030\u00b714\u2003mm; <jats:italic>P<\/jats:italic>\u2003&lt;\u20030\u00b7001). A significant negative association was found between ery\u2010apoB and IMT (\u03b2\u2003=\u2003\u22120\u00b7214: 95% CI \u22120\u00b7284 to \u22120\u00b7145; <jats:italic>P<\/jats:italic>\u2003&lt;\u20030\u00b7001). There was no association between ery\u2010apoB and plasma apoB (Pearson\u2019s <jats:italic>r<\/jats:italic>\u2003=\u2003\u22120\u00b745; <jats:italic>P<\/jats:italic>\u2003=\u20030\u00b757).<\/jats:p><jats:p><jats:bold>Conclusions\u2002<\/jats:bold> Human erythrocytes carry apoB\u2010containing lipoproteins. Subjects with atherosclerosis have lower ery\u2010apoB. High ery\u2010apoB may be protective against atherosclerosis and may reflect an alternative blood cell\u2013mediated lipoprotein transport system in the circulation, in which these lipoproteins less likely interact with the endothelium.<\/jats:p><\/jats:sec>","DOI":"10.1111\/j.1365-2362.2011.02591.x","type":"journal-article","created":{"date-parts":[[2011,8,12]],"date-time":"2011-08-12T01:12:11Z","timestamp":1313111531000},"page":"365-370","source":"Crossref","is-referenced-by-count":17,"title":["Erythrocyte\u2010associated apolipoprotein B and its relationship with clinical and subclinical atherosclerosis"],"prefix":"10.1111","volume":"42","author":[{"given":"Sarah A.","family":"Bovenberg","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Boudewijn","family":"Klop","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arash","family":"Alipour","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergio","family":"Martinez\u2010Hervas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew","family":"Westzaan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gert\u2010Jan M.","family":"van de Geijn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans W. J.","family":"Janssen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tjin","family":"Njo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erwin","family":"Birnie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rob","family":"van Mechelen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arie P.","family":"Rietveld","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan Willem F.","family":"Elte","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manuel","family":"Castro Cabezas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2011,9,13]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.81.6.1839"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.82.5.1574"},{"key":"e_1_2_6_4_2","doi-asserted-by":"crossref","first-page":"1461","DOI":"10.1016\/S0022-2275(20)38425-X","article-title":"Plasma very low density lipoproteins contain a single molecule of apolipoprotein B","volume":"29","author":"Elovson J","year":"1988","journal-title":"J Lipid Res"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(01)07098-2"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2796.2006.01643.x"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(03)12663-3"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.94.3.273"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2796.2006.01616.x"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2003-032171"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.107.159749"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.110.215434"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2760(81)90129-6"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2736(79)90145-7"},{"key":"e_1_2_6_15_2","volume-title":"ICD\u201010 2nd Edition, International Statistical Classification of Disease and Health Related Problems","author":"WHO","year":"2005"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.39335.541782.AD"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2362.2009.02234.x"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.108.163931"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1006\/pmed.2000.0715"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.atherosclerosissup.2010.04.003"},{"key":"e_1_2_6_21_2","first-page":"310","article-title":"Another function of erythrocytes: transport of circulating immune complexes","volume":"22","author":"Pascual M","year":"1995","journal-title":"Infusionsther Transfusionsmed"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI110764"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0161-5890(99)00103-0"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.109.904029"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.108.181735"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.atherosclerosis.2008.11.025"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa0707064"}],"container-title":["European Journal of Clinical Investigation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1365-2362.2011.02591.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1365-2362.2011.02591.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,31]],"date-time":"2023-10-31T18:41:45Z","timestamp":1698777705000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1365-2362.2011.02591.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,9,13]]},"references-count":26,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2012,4]]}},"alternative-id":["10.1111\/j.1365-2362.2011.02591.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1365-2362.2011.02591.x","archive":["Portico"],"relation":{},"ISSN":["0014-2972","1365-2362"],"issn-type":[{"value":"0014-2972","type":"print"},{"value":"1365-2362","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,9,13]]}}}