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We have developed a method to isolate these structures and have shown that they contain mainly membrane proteins plus a high concentration of a 60\u00a0kDa protein. The N\u2010terminal amino acid sequence of the protein is identical to that of GroEL protein. Western blotting analysis using anti\u2010GroEL antibody showed that GroEL is indeed concentrated in the vesicles. Indirect immuno\u2010fluorescence microscopy showed that GroEL protein is localized at the centre of the cells at the site of formation of FtsZ\u2010rings. Localization of GroEL is dependent on FtsZ but not other Fts proteins. GroEL mutants formed elongated cells having no or asymmetrically localized FtsZ\u2010rings at the restrictive temperature. These findings suggest a possible role of the GroEL protein in cell division.<\/jats:p>","DOI":"10.1111\/j.1365-2443.2004.00770.x","type":"journal-article","created":{"date-parts":[[2004,8,25]],"date-time":"2004-08-25T14:43:12Z","timestamp":1093444992000},"page":"765-771","source":"Crossref","is-referenced-by-count":32,"title":["FtsZ\u2010dependent localization of GroEL protein at possible division sites"],"prefix":"10.1111","volume":"9","author":[{"given":"Hidetaka","family":"Ogino","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masaaki","family":"Wachi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akihiro","family":"Ishii","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noritaka","family":"Iwai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tetsuya","family":"Nishida","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sakuo","family":"Yamada","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuo","family":"Nagai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Motoyuki","family":"Sugai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,8,25]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.155.3.983-988.1983"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.2000.4278"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1006\/jsbi.2001.4380"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.171.3.1379-1385.1989"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(73)90080-6"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-440X(00)00176-7"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.139.1.185-194.1979"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(00)80038-6"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(93)90470-B"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/45977"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.171.10.5523-5530.1989"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1119(97)00087-5"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.171.11.6379-6382.1989"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)31554-5"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.170.9.3967-3977.1988"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1989.tb08517.x"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1006\/jsbi.2001.4359"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.2000.tb00554.x"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/352036a0"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/32317"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1327.1999.00848.x"},{"key":"e_1_2_6_23_1","doi-asserted-by":"crossref","first-page":"4021","DOI":"10.1128\/jb.173.13.4021-4026.1991","article-title":"Lysis of Escherichia coli by \u03b2\u2010lactams which bind penicillin\u2010binding proteins 1a and 1b: inhibition by heat shock proteins","volume":"173","author":"Powell J.K.","year":"1991","journal-title":"J. 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