{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T06:35:11Z","timestamp":1791009311909,"version":"4.1.0"},"reference-count":54,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2006,1,20]],"date-time":"2006-01-20T00:00:00Z","timestamp":1137715200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes to Cells"],"published-print":{"date-parts":[[2006,3]]},"abstract":"<jats:p>The focal adhesion protein vinexin is a member of a family of adaptor proteins that are thought to participate in the regulation of cell adhesion, cytoskeletal reorganization, and growth factor signaling. Here, we show that vinexin \u03b2 increases the amount of and reduces the mobility on SDS\u2010PAGE of Wiskott\u2010Aldrich syndrome protein family verprolin\u2010homologous protein (WAVE) 2 protein, which is a key factor modulating actin polymerization in migrating cells. This mobility retardation disappeared after <jats:italic>in vitro<\/jats:italic> phosphatase treatment. Co\u2010immunoprecipitation assays revealed the interaction of vinexin \u03b2 with WAVE2 as well as WAVE1 and N\u2010WASP. Vinexin \u03b2 interacts with the proline\u2010rich region of WAVE2 through the first and second SH3 domains of vinexin \u03b2. Mutations disrupting the interaction impaired the ability of vinexin \u03b2 to increase the amount of WAVE2 protein. Treatments with proteasome inhibitors increased the amount of WAVE2, but did not have an additive effect with vinexin \u03b2. Inhibition of protein kinase A (PKA) activity suppressed the vinexin\u2010induced increase in WAVE2 protein, while activation of PKA increased WAVE2 expression without vinexin \u03b2. These results suggest that vinexin \u03b2 regulates the proteasome\u2010dependent degradation of WAVE2 in a PKA\u2010dependent manner.<\/jats:p>","DOI":"10.1111\/j.1365-2443.2006.00932.x","type":"journal-article","created":{"date-parts":[[2006,1,20]],"date-time":"2006-01-20T15:50:32Z","timestamp":1137772232000},"page":"281-292","source":"Crossref","is-referenced-by-count":27,"title":["Protein kinase A\u2010dependent increase in WAVE2 expression induced by the focal adhesion protein vinexin"],"prefix":"10.1111","volume":"11","author":[{"given":"Masaru","family":"Mitsushima","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takuhito","family":"Sezaki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rie","family":"Akahane","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazumitsu","family":"Ueda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiro","family":"Suetsugu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tadaomi","family":"Takenawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noriyuki","family":"Kioka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,1,20]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.50.35933"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1208614"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.33.20992"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/35025089"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200403127"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1207394"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0409376102"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M203346200"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.24.11.4979-4993.2004"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature00859"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.14.8323"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.152.3.471"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200407058"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1105"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.286.5438.309"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.43.30914"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.144.1.59"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1247\/csf.27.1"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2003.10.005"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0409120102"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(00)80231-2"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.144.5.1001"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbaexp.2004.12.004"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2443.2005.00844.x"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.39.27605"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1996.tb00917.x"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/34208"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/17.23.6932"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1093\/jb\/mvg146"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/35047107"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M402304200"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M313436200"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M107235200"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2003-04-1319"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1125"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7600586"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.18.2.872"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.15.10.5725"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.150.6.1299"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80732-1"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M103856200"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj20021539"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7600084"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2004.04.007"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1534-5807(02)00324-6"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M108644200"},{"key":"e_1_2_6_48_1","doi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