{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T06:43:30Z","timestamp":1789541010301,"version":"build-2803163510"},"reference-count":50,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2006,5,18]],"date-time":"2006-05-18T00:00:00Z","timestamp":1147910400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes to Cells"],"published-print":{"date-parts":[[2006,7]]},"abstract":"<jats:p>DNA polymerase eta (Pol\u03b7) is responsible for efficient translesion synthesis (TLS) past cis\u2010syn cyclobutane thymine dimers (TT dimers), the major DNA lesions induced by UV irradiation. Loss of human Pol\u03b7 leads to xeroderma pigmentosum variant syndrome, clearly indicating that Pol\u03b7 plays a vital role in preventing skin cancer caused by exposure to sunlight. To further examine Pol\u03b7 functions and the mechanisms that regulate this important protein, Pol\u03b7 complexes were purified from HeLa cells over\u2010expressing epitope\u2010tagged Pol\u03b7, and polypeptides associated with Pol\u03b7, including Rad18, Rad6 and Rev1, were identified by a combination of mass spectrometry and Western blot analysis. The chromatin\u2010bound fractions of cells subjected to UV irradiation, S phase synchronization, or S phase arrest were specifically enriched in such complexes. These results suggest that arrested replication forks strengthen interactions among Pol\u03b7, Rad18\/Rad6 and Rev1, consistent with the requirement for effective TLS by Pol\u03b7 at sites of DNA lesions.<\/jats:p>","DOI":"10.1111\/j.1365-2443.2006.00974.x","type":"journal-article","created":{"date-parts":[[2006,5,18]],"date-time":"2006-05-18T12:13:03Z","timestamp":1147954383000},"page":"731-744","source":"Crossref","is-referenced-by-count":53,"title":["A human DNA polymerase \u03b7 complex containing Rad18, Rad6 and Rev1; proteomic analysis and targeting of the complex to the chromatin\u2010bound fraction of cells undergoing replication fork arrest"],"prefix":"10.1111","volume":"11","author":[{"given":"Mayumi 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