{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T00:08:07Z","timestamp":1780358887751,"version":"3.54.1"},"reference-count":37,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2006,7,21]],"date-time":"2006-07-21T00:00:00Z","timestamp":1153440000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes to Cells"],"published-print":{"date-parts":[[2006,9]]},"abstract":"<jats:p>Phase resetting is one of the essential properties of circadian clocks that is required for the adjustment to a particular environment and the induction of <jats:italic>Per1<\/jats:italic> and <jats:italic>Per2<\/jats:italic> clock genes is believed to be a primary molecular event during this process. Although the intracellular signal transduction pathway underlying <jats:italic>Per1<\/jats:italic> gene activation has been well characterized, the mechanisms that control <jats:italic>Per2<\/jats:italic> up\u2010regulation have not yet been elucidated. In our present study, we demonstrate that Gq\/11 coupled receptors mediate serum\u2010induced immediate rat <jats:italic>Per2<\/jats:italic> (r<jats:italic>Per2<\/jats:italic>) transactivation in Rat\u20101 fibroblasts via intracellular Ca<jats:sup>2+<\/jats:sup> mobilization. Stimulation of these cells with a high concentration of serum was found to rapidly increase the intracellular Ca<jats:sup>2+<\/jats:sup> levels and strongly up\u2010regulated r<jats:italic>Per2<\/jats:italic> gene. r<jats:italic>Per2<\/jats:italic> induction by serum stimulation was abrogated by intracellular Ca<jats:sup>2+<\/jats:sup> chelation and depletion of intracellular Ca<jats:sup>2+<\/jats:sup> store, which suggests that the calcium mobilization is necessary for the up\u2010regulation of r<jats:italic>Per2<\/jats:italic> gene. In addition, suppression of Gq\/11 function was observed to inhibit both Ca<jats:sup>2+<\/jats:sup> mobilization and r<jats:italic>Per2<\/jats:italic> induction. Further, we demonstrated that endothelin\u2010induced acute r<jats:italic>Per2<\/jats:italic> transactivation via Gq\/11\u2010coupled endothelin receptors is also suppressed by a Gq\/11 specific inhibitor. These findings together suggest that serum and endothelin utilize a common Gq\/11\u2010PLC mediated pathway for the transactivation of r<jats:italic>Per2<\/jats:italic>, which involves the mobilization of calcium from the intracellular calcium store.<\/jats:p>","DOI":"10.1111\/j.1365-2443.2006.00999.x","type":"journal-article","created":{"date-parts":[[2006,7,22]],"date-time":"2006-07-22T01:19:18Z","timestamp":1153531158000},"page":"1039-1049","source":"Crossref","is-referenced-by-count":13,"title":["Gq\/11\u2010induced intracellular calcium mobilization mediates <i>Per2<\/i> acute induction in Rat\u20101 fibroblasts"],"prefix":"10.1111","volume":"11","author":[{"given":"Naoyuki","family":"Takashima","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atsuko","family":"Fujioka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naoto","family":"Hayasaka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayako","family":"Matsuo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Takasaki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasufumi","family":"Shigeyoshi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,7,28]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.14.6.645"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.280.5363.574"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.19-03-01115.1999"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80495-X"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.289.5488.2344"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81199-X"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(00)00758-2"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2120733"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.138.5.2049"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)80906-X"},{"key":"e_1_2_6_12_1","first-page":"H130","article-title":"Endothelin ETA receptor regulates signaling and ANF gene expression via multiple G protein\u2010linked pathways","volume":"272","author":"Hilal\u2010Dandan R.","year":"1997","journal-title":"Am. 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