{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T15:37:49Z","timestamp":1770824269529,"version":"3.50.1"},"reference-count":32,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,12,21]],"date-time":"2005-12-21T00:00:00Z","timestamp":1135123200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2006,2]]},"abstract":"<jats:p><jats:bold>Aims<\/jats:bold> :\u2002Immunohistochemistry (IHC) and fluorescence <jats:italic>in\u2010situ<\/jats:italic> hybridization (FISH) are both commonly used assays for evaluation of HER\u20102\/<jats:italic>neu<\/jats:italic> status in breast cancer. However, there is still no consensus on which method is most predictive of patient response to Herceptin<jats:sup>TM<\/jats:sup>. Recently, the automated cellular imaging system (ACIS)<jats:sup>TM<\/jats:sup> has been shown to improve the accuracy and reproducibility in scoring IHC. Our aim was to compare the results of HER\u20102\/<jats:italic>neu<\/jats:italic> expression and gene amplification in the same patients by IHC using the ACIS<jats:sup>TM<\/jats:sup> system and by FISH.<\/jats:p><jats:p><jats:bold>Methods and results<\/jats:bold> :\u2002Two hundred and forty\u2010seven breast cancer cases were studied. The concordance rate between IHC\u2010ACIS (\u2265\u20032.2) and FISH (\u2265\u20032.0) was 94%. Fifteen patients were discordant; three had borderline FISH values and three had borderline IHC values. The other nine discordant cases consisted of five IHC\u2010ACIS+, FISH\u2013 and six IHC\u2010ACIS\u2013, FISH+. HER\u20102\/<jats:italic>neu<\/jats:italic> overexpression was more common in tumours that were high\u2010grade, aneuploid, progesterone receptor and bcl\u20102 negative, with MIB\u20101 &gt;\u200a10%.<\/jats:p><jats:p><jats:bold>Conclusion<\/jats:bold> :\u2002HER\u20102\/<jats:italic>neu<\/jats:italic> assessment by the ACIS is reliable, rapid and inexpensive, and correlates highly with results obtained by FISH.<\/jats:p>","DOI":"10.1111\/j.1365-2559.2005.02322.x","type":"journal-article","created":{"date-parts":[[2005,12,21]],"date-time":"2005-12-21T15:38:14Z","timestamp":1135179494000},"page":"258-267","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":53,"title":["Comparison of immunohistochemistry by automated cellular imaging system (ACIS) versus fluorescence <i>in\u2010situ<\/i> hybridization in the evaluation of HER\u20102\/<i>neu<\/i> expression in primary breast carcinoma"],"prefix":"10.1111","volume":"48","author":[{"given":"O W","family":"Tawfik","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B F","family":"Kimler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Davis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J K","family":"Donahue","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D L","family":"Persons","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F","family":"Fan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S","family":"Hagemeister","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P","family":"Thomas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C","family":"Connor","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W","family":"Jewell","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C J","family":"Fabian","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,12,21]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0888-7543(89)90343-1"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.3798106"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.2470152"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-8049(97)10157-5"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1186\/bcr56"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1054\/brst.2000.0225"},{"key":"e_1_2_7_8_2","first-page":"1445","article-title":"Quantitative analysis of breast cancer tissue microarrays shows both high and normal levels of HER2 expression are associated with poor outcome","volume":"63","author":"Camp RL","year":"2003","journal-title":"Cancer Res."},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.11224"},{"key":"e_1_2_7_10_2","first-page":"895","article-title":"Serum HER\u20102\/neu as a prediction and monitoring parameter in a phase II study with weekly paclitaxel in metastatic breast cancer","volume":"24","author":"Luftner D","year":"2004","journal-title":"Anticancer Res."},{"key":"e_1_2_7_11_2","first-page":"63","article-title":"Detection and quantitation of HER\u20102\/neu gene amplification in human breast cancer archival material using fluorescence in situ hybridization","volume":"13","author":"Pauletti G","year":"1996","journal-title":"Oncogene"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.1996.14.3.737"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.1998.16.8.2659"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.1999.17.9.2639"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM200103153441101"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2001.19.10.2722"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2001.19.10.2587"},{"key":"e_1_2_7_18_2","doi-asserted-by":"crossref","first-page":"803","DOI":"10.5858\/2002-126-0803-CLAFHN","article-title":"Clinical laboratory assays for HER\u20102\/neu amplification and overexpression","volume":"126","author":"Cell Markers and Cytogentics Committees College of American Pathologists.","year":"2002","journal-title":"Arch. 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