{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,27]],"date-time":"2025-09-27T04:11:16Z","timestamp":1758946276766},"reference-count":32,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2006,3,28]],"date-time":"2006-03-28T00:00:00Z","timestamp":1143504000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2006,4]]},"abstract":"<jats:p><jats:bold>Aims<\/jats:bold> :\u2002In congenital diaphragmatic hernia (CDH), the pathogenesis of abnormal pulmonary morphology is still incompletely understood. Matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) are known to play an important role in the turnover of the extracellular matrix (ECM) during development and in remodelling of tissue. The aim of this study was to investigate differences in the expression of MMPs and TIMPs between CDH lungs and controls, against the background of the abnormal pulmonary vasculature in CDH.<\/jats:p><jats:p><jats:bold>Methods<\/jats:bold> :\u2002We studied 12 lungs of term CDH patients who died &lt;\u200a24\u2003h after birth and 11 normal age\u2010matched control lungs, by immunohistochemistry with antibodies against human MMP\u20101, \u20102, \u20109, TIMP\u20101 and \u20102.<\/jats:p><jats:p><jats:bold>Results<\/jats:bold> :\u2002There was a clear increase in the number of MMP\u20101\u2010reactive capillaries and fibroblasts in CDH lungs compared with controls. In contrast, TIMP\u20102 reactivity in these structures was decreased in CDH lungs. The arterial endothelium and medial smooth muscle expressed MMP\u20102, \u20109 and TIMP\u20102 in both CDH and control lungs. In small arteries (&lt;\u2003100\u2003\u00b5m in diameter), the positive surface area of MMP\u20102, \u20109 and TIMP\u20102 was significantly larger in CDH lungs than in controls. There was no difference in the distribution and expression of TIMP\u20101 between CDH lungs and normal controls.<\/jats:p><jats:p><jats:bold>Conclusion<\/jats:bold> :\u2002The differences in staining pattern of MMPs and TIMPs between normal and CDH lungs suggest that these enzymes might play a role in the abnormal remodelling of the interstitium and the pulmonary arteries in CDH lungs. This could contribute to our understanding of the abnormal lung morphology and the occurrence of pulmonary hypertension, which forms one of the major obstacles to the successful treatment of these patients.<\/jats:p>","DOI":"10.1111\/j.1365-2559.2006.02379.x","type":"journal-article","created":{"date-parts":[[2006,3,28]],"date-time":"2006-03-28T13:17:54Z","timestamp":1143551874000},"page":"588-595","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["The distribution of matrix metalloproteinases and tissue inhibitors of metalloproteinases in the lungs of congenital diaphragmatic hernia patients and age\u2010matched controls"],"prefix":"10.1111","volume":"48","author":[{"given":"K","family":"Masumoto","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J D","family":"De Rooij","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S","family":"Suita","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R","family":"Rottier","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D","family":"Tibboel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R R","family":"De Krijger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,3,28]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/1099-0496(200009)30:3<249::AID-PPUL9>3.0.CO;2-9"},{"key":"e_1_2_7_3_2","doi-asserted-by":"crossref","unstructured":"HosgorM TibboelD.Congenital diaphragmatic hernia many questions few answers.Paediatr.Respir. 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