{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,2]],"date-time":"2026-08-02T14:49:46Z","timestamp":1785682186029,"version":"3.56.0"},"reference-count":13,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2006,3,24]],"date-time":"2006-03-24T00:00:00Z","timestamp":1143158400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2006,5]]},"abstract":"Aim:\u2002<\/jats:bold> Congenital mesoblastic nephroma (CMN) is the commonest renal tumour of infancy, with classical, cellular and mixed histological subtypes described. A specific ETV6\u2010NTRK3 fusion\u2010gene product is reported in association with the cellular variant. The aim was to investigate the relationship between the presence of this product and morphological phenotype using paraffin\u2010embedded archival material.<\/jats:p>Methods and results:\u2002<\/jats:bold> Cases of CMN from a single centre during a 15\u2010year period (1989\u20131994) were identified, anonymized and blindly classified using morphological criteria. RNA was extracted from frozen and paraffin sections for both conventional reverse transcriptase\u2010polymerase chain reaction (RT\u2010PCR) and quantitative real\u2010time RT\u2010PCR. Fifteen samples were analysed; two were non\u2010informative and three expressed ETV6\u2010NTRK3 using both techniques, two showing similar expression, whilst one showed expression two orders of magnitude lower, from a cellular tumour. All fusion positive cases were previously classified as cellular subtype. Six patients had mixed\u2010subtype tumours in which the cellular components, morphologically indistinguishable from cellular tumours, were fusion negative, as were all classical cases.<\/jats:p>Conclusions:\u2002<\/jats:bold> Real\u2010time PCR Taqman assays, using both fixed and frozen tissue, provide highly reproducible detection and quantification of fusion transcript expression. Differences in expression levels may explain previous conflicting data on fusion gene detection in these tumours.<\/jats:p>","DOI":"10.1111\/j.1365-2559.2006.02400.x","type":"journal-article","created":{"date-parts":[[2006,3,24]],"date-time":"2006-03-24T14:31:49Z","timestamp":1143210709000},"page":"748-753","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":56,"title":["Expression of ETV6\u2010NTRK in classical, cellular and mixed subtypes of congenital mesoblastic nephroma"],"prefix":"10.1111","volume":"48","author":[{"given":"J","family":"Anderson","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Gibson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N J","family":"Sebire","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,4,20]]},"reference":[{"key":"e_1_2_6_2_2","first-page":"1","volume-title":"Pathology of solid tumors in children.","author":"Beckwith JB","year":"1998"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1542\/peds.40.2.272"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0046-8177(89)90156-1"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng0298-184"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)65732-X"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1535-6108(02)00180-0"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V93.4.1355"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.24.11.4636-4650.2004"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1205669"},{"key":"e_1_2_6_11_2","first-page":"8909","article-title":"The chimeric protein tyrosine kinase ETV6\u2010NTRK3 requires both Ras\u2010Erk1\/2 and PI3\u2010kinase\u2010Akt signaling for fibroblast transformation","volume":"61","author":"Tognon C","year":"2001","journal-title":"Cancer Res."},{"key":"e_1_2_6_12_2","first-page":"5046","article-title":"ETV6\u2010NTRK3 gene fusions and trisomy 11 establish a histogenetic link between mesoblastic nephroma and congenital fibrosarcoma","volume":"58","author":"Knezevich SR","year":"1998","journal-title":"Cancer Res."},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.3880006"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1097\/00043426-200208000-00014"}],"container-title":["Histopathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1365-2559.2006.02400.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1365-2559.2006.02400.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,13]],"date-time":"2023-10-13T17:20:29Z","timestamp":1697217629000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1365-2559.2006.02400.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,4,20]]},"references-count":13,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2006,5]]}},"alternative-id":["10.1111\/j.1365-2559.2006.02400.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1365-2559.2006.02400.x","archive":["Portico"],"relation":{},"ISSN":["0309-0167","1365-2559"],"issn-type":[{"value":"0309-0167","type":"print"},{"value":"1365-2559","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,4,20]]},"assertion":[{"value":"2006-04-20","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}