{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,2]],"date-time":"2025-05-02T13:30:41Z","timestamp":1746192641876},"reference-count":48,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2006,6,5]],"date-time":"2006-06-05T00:00:00Z","timestamp":1149465600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2006,8]]},"abstract":"<jats:p><jats:bold>Aims<\/jats:bold> :\u2002To investigate the immunocytochemical expression of vascular endothelial growth factor C (VEGF\u2010C) and its receptors (VEGFR\u20102 and VEGFR\u20103) in childhood acute lymphoblastic leukaemia (ALL) blasts and to determine the possible role of this complex in the pathogenesis and prognosis of ALL.<\/jats:p><jats:p><jats:bold>Methods and results<\/jats:bold> :\u2002Bone marrow samples were taken from 120 children diagnosed with ALL. An indirect immunocytochemical procedure was performed with the use of monoclonal mouse anti\u2010human antibodies against VEGF\u2010C, VEGFR\u20102 and VEGFR\u20103 (diluted 1\u2003:\u2003100). The immunocytochemical expression of VEGF\u2010C was confirmed exclusively in the cytoplasm of ALL lymphoblasts (the mean percentage was 36.4\u2003\u00b1\u20037.2). It was absent from the cytoplasm of normal haematopoietic cells in the control group. No VEGFR\u20102 or VEGFR\u20103 expression was detected in the children of either the study or control groups. The risk of induction failure or leukaemic relapse was found to be significant in all VEGF\u2010C+ patients (<jats:italic>P\u2003&lt;<\/jats:italic>\u20030.0001 and <jats:italic>P<\/jats:italic>\u2003&lt;\u20030.02, respectively; Fisher's exact test).<\/jats:p><jats:p><jats:bold>Conclusions<\/jats:bold> :\u2002The absence of VEGF\u2010C in blast cells predicts long\u2010lasting remission in all leukaemic children. Our findings also suggest that leukaemic cell invasion, following VEGF\u2010C\u2010driven lymphangiogenesis, could be related to a mediating role of this peptide produced by blast cells themselves.<\/jats:p>","DOI":"10.1111\/j.1365-2559.2006.02465.x","type":"journal-article","created":{"date-parts":[[2006,6,5]],"date-time":"2006-06-05T15:20:36Z","timestamp":1149520836000},"page":"170-177","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Vascular endothelial growth factor C\u2014a potent risk factor in childhood acute lymphoblastic leukaemia: an immunocytochemical approach"],"prefix":"10.1111","volume":"49","author":[{"given":"M","family":"Nowicki","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D","family":"Ostalska\u2010Nowicka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E","family":"Kaczmarek","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B","family":"Miskowiak","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Witt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,17]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-0348-9006-9_10"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cardiores.2004.12.002"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1469-7580.2002.00066.x"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1998.274.3.H1054"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1999.1729"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm0603-669"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/bies.20092"},{"key":"e_1_2_7_9_2","first-page":"728","article-title":"Expression of vascular endothelial growth factor and its receptors in hematopoietic malignancies","volume":"59","author":"Bellamy WT","year":"1999","journal-title":"Cancer Res."},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.1998.01076.x"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1996.tb00359.x"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.122.12.3829"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.24.14389"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.5.1988"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2559.2005.02076.x"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2004.05.003"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/84643"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.194.6.F37"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.14.8413"},{"key":"e_1_2_7_20_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.49.35172"},{"key":"e_1_2_7_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/35025220"},{"key":"e_1_2_7_22_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197111182852108"},{"key":"e_1_2_7_23_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199512283332608"},{"key":"e_1_2_7_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1040-8428(02)00135-X"},{"key":"e_1_2_7_25_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V96.2.768.014k23_768_770"},{"key":"e_1_2_7_26_2","first-page":"815","article-title":"Spectrum of tumor angiogenesis in the bone marrow of children with acute lymphoblastic leukemia","volume":"150","author":"Perez\u2010Atayde AR","year":"1997","journal-title":"Am. 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