{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,18]],"date-time":"2025-10-18T10:22:29Z","timestamp":1760782949910},"reference-count":28,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2007,5,31]],"date-time":"2007-05-31T00:00:00Z","timestamp":1180569600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2007,6]]},"abstract":"<jats:p><jats:bold>Aims:\u2002<\/jats:bold> Oestrogen is a modulator of cell growth and differentiation in the breast. It mediates most of its function through members of the oestrogen receptor (ER) family, ER\u03b1 and ER\u03b2. Lobular carcinoma <jats:italic>in situ<\/jats:italic> (LCIS) is a known risk factor for the development of breast cancer; however, the use of tamoxifen for prevention is based upon data for ER+ (ER\u03b1) LCIS associated with invasion, but limited data exist on the use of tamoxifen in cases of ER+ (ER\u03b1) LCIS occurring in the absence of invasive carcinoma. The aim of this study was to examine ER expression in LCIS to determine the relationship of ER\u03b1 to ER\u03b2 and, thereby, to determine whether it is of clinical value to measure ER\u03b2 along with ER\u03b1.<\/jats:p><jats:p><jats:bold>Methods and results:\u2002<\/jats:bold> Core biopsy specimens from 50 patients were examined retrospectively. Histology was reviewed and histological parameters were assessed. Formalin\u2010fixed paraffin\u2010embedded tissue was available for E\u2010cadherin, ER\u03b1 and ER\u03b2 immunohistochemistry. The degree of ER\u03b1 and ER\u03b2 nuclear reactivity was quantified. The patients' mean age was 55\u2003years. The mean follow\u2010up duration was 48\u2003months. All 50 cases of LCIS were E\u2010cadherin\u2010negative. All cases were ER\u03b1+ and ER\u03b2+. The staining intensity of ER\u03b2 was strong and included staining of periductal stromal cells. The median percentage of cells immunoreactive for ER\u03b1 was 75% and for ER\u03b2 70% (range 10% weak positive to 100% strong positive). There was a statistically significant relationship between ER\u03b2 staining intensity and incidence of ipsilateral breast cancer (<jats:italic>P\u2003=<\/jats:italic>\u20030.010).<\/jats:p><jats:p><jats:bold>Conclusions:\u2002<\/jats:bold> The presence and intensity of both stromal and glandular ER\u03b2 immunoreactivity suggest that the action of oestrogen on LCIS is on both stromal and glandular cells. Future studies are needed to determine whether the presence of ER\u03b2 in LCIS could be targeted to influence the treatment of this disease and perhaps alter its natural history.<\/jats:p>","DOI":"10.1111\/j.1365-2559.2007.02689.x","type":"journal-article","created":{"date-parts":[[2007,6,1]],"date-time":"2007-06-01T06:12:44Z","timestamp":1180678364000},"page":"875-880","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Expression of ER\u03b1 and ER\u03b2 in lobular carcinoma <i>in situ<\/i>"],"prefix":"10.1111","volume":"50","author":[{"given":"L P","family":"Middleton","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G H","family":"Perkins","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S L","family":"Tucker","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A A","family":"Sahin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S E","family":"Singletary","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,5,31]]},"reference":[{"key":"e_1_2_6_2_2","first-page":"4669","article-title":"Estrogen receptor as an independent prognostic factor for early recurrence in breast cancer","volume":"37","author":"Knight WA","year":"1977","journal-title":"Cancer Res."},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-4731(75)90062-X"},{"key":"e_1_2_6_4_2","first-page":"109","article-title":"Specific receptors of the steroid hormones in breast cancer","volume":"1","author":"Wittlift JL","year":"1974","journal-title":"Semin. 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