{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T22:35:41Z","timestamp":1792190141435,"version":"4.4.0"},"reference-count":43,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2008,7,22]],"date-time":"2008-07-22T00:00:00Z","timestamp":1216684800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2008,8]]},"abstract":"<jats:p><jats:bold>Aims:\u2002<\/jats:bold> To assess the expression profile of the activated form of signal transducer and activator of transcription (Stat)3 in gestational trophoblastic disease (GTD) and correlate the findings with clinicopathological parameters.<\/jats:p><jats:p><jats:bold>Methods and results:\u2002<\/jats:bold> By immunohistochemistry, both cytoplasmic and nuclear expression of p\u2010Stat3\u2010Ser<jats:sup>727<\/jats:sup> was demonstrated in 88 trophoblastic tissues, including placentas and GTD. Nuclear immunoreactivity of p\u2010Stat3\u2010Ser<jats:sup>727<\/jats:sup> was significantly higher in hydatidiform mole (HM) (<jats:italic>P<\/jats:italic>\u2003&lt;\u20030.001) and choriocarcinoma (<jats:italic>P<\/jats:italic>\u2003=\u20030.009) when compared with normal placentas. Placental site trophoblastic tumours (PSTT) and epithelioid trophoblastic tumours (ETT) also demonstrated higher nuclear p\u2010Stat3\u2010Ser<jats:sup>727<\/jats:sup> expression than their normal trophoblast counterparts. Higher p\u2010Stat3\u2010Ser<jats:sup>727<\/jats:sup> expression was confirmed in choriocarcinoma cell lines, JEG\u20103 and JAR, than in a normal trophoblast cell line, with both nuclear and cytoplasmic fractions demonstrated by immunoblotting. Spontaneously regressed HM showed significantly increased nuclear and cytoplasmic p\u2010Stat3\u2010Ser<jats:sup>727<\/jats:sup> immunoreactivity over those that developed gestational trophoblastic neoplasia (GTN) (<jats:italic>P<\/jats:italic>\u2003=\u20030.013, <jats:italic>P<\/jats:italic>\u2003=\u20030.039). There was a significant positive and inverse correlation between nuclear p\u2010Stat3\u2010Ser<jats:sup>727<\/jats:sup> immunoreactivity and apoptotic indices [terminal deoxynucleotidyl transferase (TdT)\u2010mediated deoxyuridine triphosphate (dUTP) nick end labelling and M30 CytoDeath antibody] (<jats:italic>P<\/jats:italic>\u2003=\u20030.001, <jats:italic>P<\/jats:italic>\u2003&lt;\u20030.001, Spearman\u2019s \u03c1 test) and Bcl\u20102 expression (<jats:italic>P<\/jats:italic>\u2003=\u20030.034), respectively.<\/jats:p><jats:p><jats:bold>Conclusions:\u2002<\/jats:bold> p\u2010Stat3\u2010Ser<jats:sup>727<\/jats:sup> plays a role in the pathogenesis of GTD, probably through the regulation of apoptosis. p\u2010Stat3\u2010Ser<jats:sup>727<\/jats:sup> immunoreactivity is a potential marker in predicting GTN in HM.<\/jats:p>","DOI":"10.1111\/j.1365-2559.2008.03089.x","type":"journal-article","created":{"date-parts":[[2008,7,22]],"date-time":"2008-07-22T17:27:49Z","timestamp":1216747669000},"page":"139-146","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Activated Stat3 expression in gestational trophoblastic disease: correlation with clinicopathological parameters and apoptotic indices"],"prefix":"10.1111","volume":"53","author":[{"given":"H Y","family":"Chan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M K Y","family":"Siu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H J","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E S Y","family":"Wong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H Y S","family":"Ngan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K Y K","family":"Chan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A N Y","family":"Cheung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2008,7,22]]},"reference":[{"key":"e_1_2_7_2_2","first-page":"1359","volume-title":"Haines and Taylor obstetrical and gynaecological patholog","author":"Paradinas FJ","year":"2003"},{"key":"e_1_2_7_3_2","first-page":"1193","volume-title":"Blaustein\u2019s pathology of the female genital trac","author":"Shih IM","year":"2002"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1525-1438.1994.04010036.x"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1521-6934(03)00094-4"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.8197455"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.64.070195.003201"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.277.5332.1630"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1203476"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.12.13.2048"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1378.063"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.02404-06"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1203527"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.10364"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1007\/s000180050394"},{"key":"e_1_2_7_16_2","first-page":"23","article-title":"Elevated phosphorylation of AKT and Stat3 in prostate, breast, and cervical cancer cells","volume":"17","author":"Page C","year":"2000","journal-title":"Int. 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