{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,27]],"date-time":"2025-09-27T21:59:28Z","timestamp":1759010368341},"reference-count":34,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2010,2,22]],"date-time":"2010-02-22T00:00:00Z","timestamp":1266796800000},"content-version":"vor","delay-in-days":21,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2010,2]]},"abstract":"<jats:p>Obana Y, Sano M, Jike T, Homma T &amp; Nemoto N\u2028(2010) <jats:italic>Histopathology<\/jats:italic><jats:bold>56,<\/jats:bold> 372\u2013383<\/jats:p><jats:p> <jats:bold>Differential diagnosis of trichosporonosis using conventional histopathological stains and electron microscopy<\/jats:bold> <\/jats:p><jats:p><jats:bold>Aims:\u2002<\/jats:bold> Although <jats:italic>Trichosporon<\/jats:italic> is a causative pathogen of white piedra and summer\u2010type hypersensitivity pneumonitis, fatal disseminated trichosporonosis cases have recently been increasing. However, <jats:italic>Trichosporon<\/jats:italic> is often confused with other fungi, especially <jats:italic>Candida<\/jats:italic>, in pathological specimens. The aim was to determine the utility of histopathological stains and electron microscopy for diagnosing trichosporonosis.<\/jats:p><jats:p><jats:bold>Methods and results:\u2002<\/jats:bold> Autopsy cases of trichosporonosis, candidiasis, aspergillosis and cryptococcosis were investigated using histopathological stains and electron microscopy. Using Grocott\u2019s method, <jats:italic>Trichosporon<\/jats:italic> was weakly detected compared with other fungi. In contrast, diluted periodic acid methenamine silver (PAM) stain clearly enhanced the intensity of staining of <jats:italic>Trichosporon<\/jats:italic> compared with <jats:italic>Candida<\/jats:italic>. Furthermore, Alcian blue and colloidal iron stains predominantly detected <jats:italic>Trichosporon<\/jats:italic>. Electron microscopy after staining with diluted PAM demonstrated that <jats:italic>Trichosporon<\/jats:italic> has a variety of hyphal sizes and laminar deposition of rough silver granules, whereas <jats:italic>Candida<\/jats:italic> has uniform pseudohyphae and fine granules. The average diameter and population area of the granules were significantly higher in <jats:italic>Trichosporon<\/jats:italic> compared with <jats:italic>Candida<\/jats:italic> (<jats:italic>P<\/jats:italic>\u2003&lt;\u20030.01). Meanwhile, the laminar structure was preserved in the cell walls of <jats:italic>Trichosporon<\/jats:italic> without silver stains, whereas a low\u2010density structure was observed in <jats:italic>Candida<\/jats:italic>.<\/jats:p><jats:p><jats:bold>Conclusions:\u2002<\/jats:bold> Histopathological staining patterns and electron microscopic findings can facilitate the diagnosis of trichosporonosis.<\/jats:p>","DOI":"10.1111\/j.1365-2559.2010.03477.x","type":"journal-article","created":{"date-parts":[[2010,2,22]],"date-time":"2010-02-22T11:58:49Z","timestamp":1266839929000},"page":"372-383","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Differential diagnosis of trichosporonosis using conventional histopathological stains and electron microscopy"],"prefix":"10.1111","volume":"56","author":[{"given":"Yukari","family":"Obana","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Makoto","family":"Sano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toyoharu","family":"Jike","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Taku","family":"Homma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Norimichi","family":"Nemoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,2,22]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1439-0507.2003.00834.x"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-3062.2005.00104.x"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/147.2.199"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1378\/chest.108.1.190"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bmt.1702231"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.4.1818-1828.2005"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1080\/00365540410017563"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/s005200050221"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/41.3.373"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1348-0421.2001.tb01282.x"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.mph.0000164865.70522.d7"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1086\/505970"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.2.911.2003"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.4.1144-1146.2002"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.10.4419-4431.2004"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1086\/500323"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1007\/s15010-007-6278-6"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00199390"},{"key":"e_1_2_7_20_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1699-0463.1996.tb00714.x"},{"key":"e_1_2_7_21_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01606321"},{"key":"e_1_2_7_22_2","first-page":"191","article-title":"Disseminated Trichosporon infection. 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